<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. The objective of this work was to apply the real time PCR assay for the detection of CEVd in samples collected from symptomatic CEVd-infected plants in Mitidja (North Algeria). The assay showed an excellent diagnostic specificity where 38 out of 50 samples showed a positive reaction for CEVd, which revealed the presence of CEVd in citrus orchards with a prevalence of 76%. Consequently, this work offers a quick alternative to conventional methods for the early diagnosis and the prevalence assessment of CEVd.</p>
</abstract>

Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. The objective of this work was to apply the real time PCR assay for the detection of CEVd in samples collected from symptomatic CEVd-infected plants in Mitidja (North Algeria). The assay showed an excellent diagnostic specificity where 38 out of 50 samples showed a positive reaction for CEVd, which revealed the presence of CEVd in citrus orchards with a prevalence of 76%. Consequently, this work offers a quick alternative to conventional methods for the early diagnosis and the prevalence assessment of CEVd.

Application of Real Time PCR for the Prevalence Determination of Citrus Exocortis Viroid (CEVd) in Algeria

Département de Biologie, Faculté des Sciences de la Nature et de la Vie

Laboratoire de Protection et Valorisation des Ressources Agrobiologiques, Faculté des Sciences de la Nature et de la Vie

Laboratoire de Valorisation et Conservation des Ecosystèmes Arides (LVCEA), Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre

Laboratoire de Biologie des Systèmes Microbiens

Department of Life and Earth Sciences, Faculty of Sciences

Département de Biologie, Faculté des Sciences et Technologie

Département de Génie des Procédés, Faculté de Technologie

Advanced Research in Life Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to...

Application of Real Time PCR for the Prevalence Determination of Citrus Exocortis Viroid (CEVd) in Algeria

Data publikacji: 11 kwi 2023

Zakres stron: 68 - 72

Otrzymano: 01 lut 2023

Przyjęty: 01 kwi 2023

DOI: https://doi.org/10.2478/arls-2023-0008

Słowa kluczowe
Citrus Exocortis Viroid (CEVd), Real Time PCR, Diagnosis

© 2023 Ahlem El Ferran et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Application of Real Time PCR for the Prevalence Determination of Citrus Exocortis Viroid (CEVd) in Algeria

Data publikacji: 11 kwi 2023

Zakres stron: 68 - 72

Otrzymano: 01 lut 2023

Przyjęty: 01 kwi 2023

DOI: https://doi.org/10.2478/arls-2023-0008

Słowa kluczoweCitrus Exocortis Viroid (CEVd), Real Time PCR, Diagnosis

© 2023 Ahlem El Ferran et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
Citrus Exocortis Viroid (CEVd), Real Time PCR, Diagnosis