Treponema denticola | Fusobacterium nucleatum | Prevotella intermedia | Cycles | Reading melt curve | |
---|---|---|---|---|---|
Desn. initial | 96°C-5 m | 96°C-5 m | 96°C-5 m | 30 | 80°C |
Desn. nat | 95°C-30 s | 95°C-30 s | 95°C-30 s | ||
Align | 55°C-45 s | 56°C-45 s | 56°C-45 s | ||
Ext | 72°C-45 s | 72°C-45 s | 72°C-45 s | ||
Ext final | 72°C-10 m | 72°C-10 m | 72°C-10 m | ||
Conservation | 4°C | 4°C | 4°C |
Specific initiators | Amplified products (pb) | Bacteria |
---|---|---|
FTd 5′-TAATACCGAATGTGCTCATTTACAT-3′ | 316 | |
RTd 5′-TCAAAGAAGCATTCCCTCTTCTTCTTA-3; | ||
FFn 5′-AGAGTTTGATCCTGGCTCAG-3′ | 408 | |
RFn 5′-GTCATCGTGCACACAGAATTGCTG-3 | ||
FPi 5′-TTTGTTGGGGAGTAAAGCGGG-3′ | 576 | |
RPi 5′-TCAACATCTCTGTATCCTGCGT-3′ |