<abstract xmlns="http://www.w3.org/1999/xhtml">

Neem (<italic>Azadirachta indica</italic>) belongs to <italic>Meliaceae</italic> family, represented mainly by trees, and widely cultivated and adapted in many tropical regions. The objective of this study was to evaluate the effect of increasing doses of neem seed oil (NSO) on ruminal methane (CH4) formation, diet degradability, and fermentation characteristics after 24 h of <italic>in vitro</italic> incubation. Treatments were randomly designed to four doses of NSO supplemented to the basal diet (0, 20, 40, or 60 ml/kg DM). Increasing NSO dose resulted in a quadratic decrease (P &lt; 0.05) in net gas (expressed as ml/g DM and ml/g TDOM) and CH4 (expressed as ml/g TDNDF) production, while CH4 (expressed as ml/g TDOM), acetate and propionate proportions decreased linearly confirming a dose-related effect. A quadratic increase in TDOM and linear increase (P = 0.023) in DNDF, NH3-N concentrations, and total protozoal counts were observed. However, a linear increase (P = 0.009) was found in the ruminal butyrate proportion and partitioning factor as dietary NSO supplementation increased. In conclusion, dietary NSO supplementation mediated some desirable fermentation patterns, reducing ruminal NH3-N concentration and CH4 production with some adverse effects on fiber degradability. However, practical research under long-term conditions is required for further investigation.
</abstract>

Neem (Azadirachta indica) belongs to Meliaceae family, represented mainly by trees, and widely cultivated and adapted in many tropical regions. The objective of this study was to evaluate the effect of increasing doses of neem seed oil (NSO) on ruminal methane (CH4) formation, diet degradability, and fermentation characteristics after 24 h of in vitro incubation. Treatments were randomly designed to four doses of NSO supplemented to the basal diet (0, 20, 40, or 60 ml/kg DM). Increasing NSO dose resulted in a quadratic decrease (P < 0.05) in net gas (expressed as ml/g DM and ml/g TDOM) and CH4 (expressed as ml/g TDNDF) production, while CH4 (expressed as ml/g TDOM), acetate and propionate proportions decreased linearly confirming a dose-related effect. A quadratic increase in TDOM and linear increase (P = 0.023) in DNDF, NH3-N concentrations, and total protozoal counts were observed. However, a linear increase (P = 0.009) was found in the ruminal butyrate proportion and partitioning factor as dietary NSO supplementation increased. In conclusion, dietary NSO supplementation mediated some desirable fermentation patterns, reducing ruminal NH3-N concentration and CH4 production with some adverse effects on fiber degradability. However, practical research under long-term conditions is required for further investigation.

Potential of graded doses of neem (<italic>Azadirachta indica</italic>) seed oil on ruminal fermentation characteristics, degradability, and methane formation <italic>in vitro</italic>

Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences

Department of Animal and Fish Production, Faculty of Agriculture

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Potential of graded doses of neem () seed oil on ruminal fermentation characteristics, degradability, and methane formation 

Neem (Azadirachta indica) belongs to Meliaceae family, represented mainly by trees, and widely cultivated and adapted in many tropical regions. The objective...

Potential of graded doses of neem (Azadirachta indica) seed oil on ruminal fermentation characteristics, degradability, and methane formation in vitro

Data publikacji: 19 lip 2022

Zakres stron: 993 - 999

Otrzymano: 29 kwi 2021

Przyjęty: 03 wrz 2021

DOI: https://doi.org/10.2478/aoas-2021-0073

Słowa kluczowe
neem seed oil, rumen fermentation, feed degradability, methanogenesis

© 2021 Waleed Al-Marzooqi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Potential of graded doses of neem (Azadirachta indica) seed oil on ruminal fermentation characteristics, degradability, and methane formation in vitro

Data publikacji: 19 lip 2022

Zakres stron: 993 - 999

Otrzymano: 29 kwi 2021

Przyjęty: 03 wrz 2021

DOI: https://doi.org/10.2478/aoas-2021-0073

Słowa kluczoweneem seed oil, rumen fermentation, feed degradability, methanogenesis

© 2021 Waleed Al-Marzooqi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Słowa kluczowe
neem seed oil, rumen fermentation, feed degradability, methanogenesis