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Effects of concomitant use of THC and irinotecan on tumour growth and biochemical markers in a syngeneic mouse model of colon cancer

Suzana Žunec
Suzana Žunec
, 
Irena Brčić Karačonji
Irena Brčić Karačonji
, 
Martin Čatalinac
Martin Čatalinac
, 
Andreja Jurič
Andreja Jurič
, 
Anja Katić
Anja Katić
, 
Goran Kozina
Goran Kozina
, 
Vedran Micek
Vedran Micek
, 
Marijana Neuberg
Marijana Neuberg
 oraz 
Ana Lucić Vrdoljak
Ana Lucić Vrdoljak
   | 30 wrz 2023
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Article Category: Original article
Data publikacji: 30 wrz 2023
Zakres stron: 198 - 206
Otrzymano: 01 lip 2023
Przyjęty: 01 wrz 2023
DOI: https://doi.org/10.2478/aiht-2023-74-3765
© 2023 Suzana Žunec et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Experimental design. s.c. – subcutaneous; i.p. – intraperitoneal; p.o. – per os; TC – tumour control; IRI – irinotecan; THC – Δ9-tetrahydrocannabinol
Experimental design. s.c. – subcutaneous; i.p. – intraperitoneal; p.o. – per os; TC – tumour control; IRI – irinotecan; THC – Δ9-tetrahydrocannabinol

Figure 2

Changes in mouse body weight following treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values represent the mean of five mice per group.
Changes in mouse body weight following treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values represent the mean of five mice per group.

Figure 3

Changes in average tumour volume in syngeneic colorectal mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values are expressed as means ± SEM of five mice per group. a significantly different vs IRI (p<0.05).
Changes in average tumour volume in syngeneic colorectal mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values are expressed as means ± SEM of five mice per group. a significantly different vs IRI (p<0.05).

Figure 4

Changes in total cholinesterase (ChE), acetylcholinesterase (AChE), and butyrylcholinesterase activities (BChE) in the whole blood of syngeneic colorectal cancer mice after treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Results are presented as medians and interquartile ranges. * significantly different from control (Kruskal-Wallis test, p<0.05)
Changes in total cholinesterase (ChE), acetylcholinesterase (AChE), and butyrylcholinesterase activities (BChE) in the whole blood of syngeneic colorectal cancer mice after treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Results are presented as medians and interquartile ranges. * significantly different from control (Kruskal-Wallis test, p<0.05)

Figure 5

Changes in oxidative stress parameters in the plasma and erythrocytes of syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control, TC). (a) The levels of reactive oxygen species (ROS) in the plasma; (b) the levels of glutathione (GSH) in the plasma; (c) concentrations of thiobarbituric reactive substances (TBARS) in the plasma; (d) erythrocyte catalase (CAT) activity; (e) erythrocyte superoxide dismutase (SOD) activity; (f) erythrocyte glutathione peroxidase (GPx) activity. * significantly higher than TC (p<0.05); a significantly higher than IRI (p<0.05); b significantly higher than THC (p<0.05)
Changes in oxidative stress parameters in the plasma and erythrocytes of syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control, TC). (a) The levels of reactive oxygen species (ROS) in the plasma; (b) the levels of glutathione (GSH) in the plasma; (c) concentrations of thiobarbituric reactive substances (TBARS) in the plasma; (d) erythrocyte catalase (CAT) activity; (e) erythrocyte superoxide dismutase (SOD) activity; (f) erythrocyte glutathione peroxidase (GPx) activity. * significantly higher than TC (p<0.05); a significantly higher than IRI (p<0.05); b significantly higher than THC (p<0.05)

Relative brain and liver weights in syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control - TC)

Relative weight (%)
TC IRI THC IRI + THC
Brain 1.42±0.05 1.48±0.12 1.36±0.04 1.35±0.09
Liver 5.37±0.11 5.36±0.21 5.24±0.09 5.66±0.24
eISSN:
1848-6312
Języki:
Angielski, Slovenian
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Medicine, Basic Medical Science, other
Kanał RSS czasopisma