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Kidney cell DNA damage caused by combined exposure to volatile anaesthetics and 1 Gy or 2 Gy radiotherapy dose in vivo

Vesna Benković
Vesna Benković
, 
Nada Oršolić
Nada Oršolić
, 
Anica Horvat Knežević
Anica Horvat Knežević
, 
Nikola Borojević
Nikola Borojević
, 
Gordana Brozović
Gordana Brozović
 oraz 
Mirta Milić
Mirta Milić
   | 07 kwi 2022
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Article Category: Original article
Data publikacji: 07 kwi 2022
Zakres stron: 62 - 70
Otrzymano: 01 paź 2021
Przyjęty: 01 mar 2022
DOI: https://doi.org/10.2478/aiht-2022-73-3600
© 2022 Vesna Benković, Nada Oršolić, Anica Horvat Knežević, Nikola Borojević, Gordana Brozović, Mirta Milić, published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Tail length and tail intensity in kidney cells of control (C) Swiss albino mice and mice subjected to two-hour anaesthesia with halothane (H), sevoflurane (S), or isoflurane (I) at 0, 2, 6, and 24 h after anaesthesia (based on the total score of 200 comets per group (40 comets per animal)
Tail length and tail intensity in kidney cells of control (C) Swiss albino mice and mice subjected to two-hour anaesthesia with halothane (H), sevoflurane (S), or isoflurane (I) at 0, 2, 6, and 24 h after anaesthesia (based on the total score of 200 comets per group (40 comets per animal)

Figure 2

Tail length and tail intensity in kidney cells of Swiss albino mice exposed to halothane (H), sevoflurane (S), or isoflurane (I) and/or gamma-irradiation (60Co) of 1 Gy 0, 2, 6, and 24 h after irradiation [(based on the total score of 200 comets per group (40 comets per animal)]
Tail length and tail intensity in kidney cells of Swiss albino mice exposed to halothane (H), sevoflurane (S), or isoflurane (I) and/or gamma-irradiation (60Co) of 1 Gy 0, 2, 6, and 24 h after irradiation [(based on the total score of 200 comets per group (40 comets per animal)]

Figure 3

Tail length and tail intensity in kidney cells of Swiss albino mice exposed to halothane (H), sevoflurane (S), or isoflurane (I) and/or gamma-irradiation (60Co) of 2 Gy 0, 2, 6, and 24 h after irradiation [(based on the total score of 200 comets per group (40 comets per animal)]
Tail length and tail intensity in kidney cells of Swiss albino mice exposed to halothane (H), sevoflurane (S), or isoflurane (I) and/or gamma-irradiation (60Co) of 2 Gy 0, 2, 6, and 24 h after irradiation [(based on the total score of 200 comets per group (40 comets per animal)]

Statistical differences (Mann–Whitney U test; p<0.05), in tail length (TL, μm) and tail intensity (TI, % of DNA in comet tail) in kidney cells of Swiss albino mice treated with halothane, sevoflurane, or isoflurane alone or in combination with 1 or 2 Gy gamma-irradiation (60Co) compared to corresponding controls at the same time point

Non-irradiated
Exposure Halothane Sevoflurane Isoflurane
Time points TL TI TL TI TL TI
0 h NS <0.001 <0.001 <0.001 <0.001 0.004
2 h <0.001 <0.001 0.025 <0.001 NS NS
6 h <0.001 <0.001 <0.001 0.01 0.021 NS
24 h <0.001 <0.001 NS <0.001 NS <0.001
Irradiated 1 Gy
Exposure Halothane + 1 Gy Sevoflurane + 1 Gy Isoflurane + 1 Gy
Time points TL TI TL TI TL TI
0 h <0.001 <0.001 NS NS NS 0.014
2 h <0.001 <0.001 <0.001 NS <0.001 NS
6 h <0.001 NS <0.001 0.006 NS NS
24 h <0.001 0.004 0.001 NS <0.001 0.003
Irradiated 2 Gy
Exposure Halothane + 2 Gy Sevoflurane + 2 Gy Isoflurane + 2 Gy
Time points TL TI TL TI TL TI
0 h <0.001 <0.001 <0.001 <0.001 <0.001 NS
2 h <0.001 0.002 NS <0.001 <0.001 0.002
6 h <0.001 NS <0.001 NS 0.02 NS
24 h <0.001 <0.001 <0.001 NS <0.001 <0.001

Distribution of 240 male Swiss albino mice by study groups

Control Halothane (2.4 % v/v) Sevoflurane (2.4 % v/v) Isoflurane (1.7 % v/v)
Non-irradiated
Time points 0 h 0 h 0 h 0 h
2 h 2 h 2 h 2 h
6 h 6 h 6 h 6 h
24 h 24 h 24 h 24 h
Irradiated with 1 Gy
Time points 2 h 2 h 2 h 2 h
0 h 0 h 0 h 0 h
6 h 6 h 6 h 6 h
24 h 24 h 24 h 24 h
Irradiated with 2 Gy
Time points 0 h 0 h 0 h 0 h
2 h 2 h 2 h 2 h
6 h 6 h 6 h 6 h
24 h 24 h 24 h 24 h

Cellular DNA repair index (percentage of repair) of tail length and tail intensity in non-irradiated mice and mice exposed to halothane (H), sevoflurane (S), or isoflurane (I) and/or 1 Gy or 2 Gy 2, 6, and 24 h from irradiation

Tail length Tail intensity
2 h 6 h 24 h 2 h 6 h 24 h
Non-irradiated
Halothane -17.86 -12.11 3.56 10.32 28.42 -6.44
Sevoflurane -2.56 16.14 12.39 -15.71 -0.65 -10.11
Isoflurane -10.71 -3.42 -15.28 26.77 28.12 -15.62
Irradiated with 1 Gy
1 Gy 3.76 12.01 -18.10 24.20 -58.95 -25.08
Halothane + 1Gy -9.78 -1.46 -7.86 15.28 2.54 -7.38
Sevoflurane + 1Gy 16.06 -2.07 1.76 3.35 11.02 -10.80
Iisoflurane + 1Gy 14.92 15.91 7.34 43.96 4.92 36.08
Irradiated with 2 Gy
2 Gy -28.19 -17.81 -11.22 -29.75 -90.54 -40.43
Halothane + 2Gy -16.54 -19.53 5.97 25.87 18.70 27.56
Sevoflurane + 2Gy 1.44 1.59 3.09 -20.68 -7.65 24.16
Isoflurane + 2Gy 15.54 2.52 -5.55 39.13 -77.69 -87.18
eISSN:
1848-6312
Języki:
Angielski, Slovenian
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Medicine, Basic Medical Science, other
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