Pro-oxidant potency of clothianidin in rainbow trout

Tarek Fakhereddin; Demet Doğan

Otwarty dostęp

Pro-oxidant potency of clothianidin in rainbow trout

Tarek Fakhereddin

oraz

Demet Doğan

| 28 cze 2021

Archives of Industrial Hygiene and Toxicology

Tom 72 (2021): Zeszyt 2 (June 2021)

O artykule

Poprzedni artykuł

Następny artykuł

Zacytuj

Udostępnij

Article Category: Original article

Data publikacji: 28 cze 2021

Zakres stron: 164 - 172

Otrzymano: 01 sty 2021

Przyjęty: 01 maj 2021

DOI: https://doi.org/10.2478/aiht-2021-72-3522

© 2021 Tarek Fakhereddin and Demet Doğan, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Change in mean SOD, CAT, and GPx enzyme activities in heart and spleen tissues of rainbow trout following clothianidin exposure with respect to control. * p<0.05; **p<0.01

Change in mean AChE enzyme activity in heart and spleen tissues of rainbow trout following clothianidin exposure with respect to control. * p<0.05; **p<0.01

Effects of environmental clothianidin exposure on protein (mg/mL), GSH (nmol/mg protein), protein carbonyl (ng/mg protein), and MDA (nmol/mg protein) levels in heart and spleen tissues of rainbow trout

	Heart			Spleen
	Exposure duration (days)
	7	14	21	7	14	21
Protein
Control	5.35±0.27^ax	5.63±0.39^ax	5.15±0.48^ax	6.85±0.78^ax	6.30±0.20^ax	7.15±0.85^ax
3 μg/L	5.45±0.28^ax	5.20±0.47^ax	4.75±0.30^ax	6.93±0.60^ax	6.20±0.70^ax	5.43±0.12^abx
15 μg/L	5.60±0.28^ax	5.15±0.39^axy	4.50±0.27^ay	7.28±0.92^ax	4.10±0.34^by	4.55±0.31^by
30 μg/L	5.38±0.36^ax	4.63±0.52^ax	2.65±0.41^by*	6.45±0.78^ax	3.67±0.57^by*	3.78±0.36^by
GSH
Control	0.205±0.010^ax	0.244±0.017^ax	0.219±0.021^ax	0.257±0.031^ax	0.311±0.021^ax	0.313±0.035^ax
3 μg/L	0.210±0.012^ax	0.204±0.020^ax	0.237±0.013^ax	0.160±0.024^ax	0.320±0.035^ay	0.322±0.010^ay
15 μg/L	0.217±0.010^ax	0.195±0.015^ax	0.292±0.017^ax	0.173±0.020^ax	0.308±0.022^ay	0.267±0.038^axy
30 μg/L	0.217±0.016^ax	0.310±0.041^ax	0.692±0.103^by*	0.237±0.026^ax	0.591±0.075^by*	0.387±0.030^az
Protein carbonyl
Control	0.064±0.003^ax	0.077±0.005^ax	0.080±0.008^ax	0.205±0.021^ax	0.229±0.023^ax	0.207±0.018^ax
3 μg/L	0.077±0.005^abx	0.081±0.008^ax	0.068±0.008^ax	0.171±0.022^ax	0.192±0.009^ax	0.194±0.017^ax
15 μg/L	0.079±0.004^abx	0.060±0.005^ax	0.068±0.004^ax	0.174±0.016^ax	0.190±0.024^ax	0.268±0.015^bx*
30 μg/L	0.109±0.008^bx	0.130±0.017^by*	0.227±0.035^bz*	0.205±0.024^ax	0.302±0.019^by*	0.323±0.021^cz*
MDA
Control	0.100±0.004^ax	0.128±0.021^ax	0.149±0.012^ax	0.428±0.051^ax	0.470±0.032^ax	0.376±0.044^ax
3 μg/L	0.153±0.009^abx	0.216±0.010^abx	0.194±0.011^ax	0.310±0.025^ax	0.353±0.038^ax	0.506±0.013^by
15 μg/L	0.144±0.006^ax	0.178±0.006^abx	0.189±0.010^ax	0.374±0.017^ax	0.359±0.026^ax	0.540±0.034^by*
30 μg/L	0.250±0.018^bx*	0.231±0.030^bx	0.701±0.108^by*	0.338±0.038^ax	0.722±0.095^by*	0.612±0.044^by*

Heart and spleen somatic indices in rainbow trout by weeks of exposure to clothianidin

		Somatic indices
	7 days	14 days	21 days
Heart
Control	0.119±0.015^ax	0.126±0.008^ax	0.123±0.008^ax
3 μg/L	0.139±0.005^ax	0.111±0.012^ax	0.137±0.014^ax
15 μg/L	0.155±0.030^ax	0.149±0.021^ax	0.158±0.024^ax
30 μg/L	0.125±0.004^ax	0.147±0.019^axy	0.178±0.013^by
Spleen
Control	0.204±0.053^ax	0.225±0.048^ax	0.206±0.010^ax
3 μg/L	0.172±0.037^ax	0.147±0.035^ax	0.106±0.022^abx
15 μg/L	0.287±0.065^ax	0.154±0.030^ax	0.113±0.012^abx
30 μg/L	0.197±0.036^ax	0.214±0.055^ax	0.092±0.009^bx

Pearson correlation coefficients for heart and spleen tissue biomarkers in rainbow trout following clothianidin exposure

		Protein	carbonyl Protein	MDA	AChE	SOD	CAT	GPx	GSH
Heart	Protein	1
	Protein Carbonyl	-0.788^*	1
	MDA	-0.760^*	0.901^*	1
	AChE	0.516^*	-0.633^*	-0.654^*	1
	SOD	-0.816^*	0.874^*	0.930^*	-0.582^*	1
	CAT	-0.671^*	0.781^*	0.809^*	-0.437^*	0.735^*	1
	GPx	-0.874^*	0.849^*	0.937^*	-0.598^*	0.951^*	0.798^*	1
	GSH	-0.855^*	0.908^*	0.942^*	-0.624^*	0.965^*	0.792^*	0.969^*	1
Spleen	Protein	1
	Protein Carbonyl	-0.526^*	1
	MDA	-0.705^*	0.650^*	1
	AChE	-0.254	0.225	0.218	1
	SOD	-0.765^*	0.505^*	0.758^*	0.265	1
	CAT	-0.736^*	0.647^*	0.749^*	0.141	0.725^*	1
	GPx	-0.789^*	0.589^*	0.818^*	0.239	0.924^*	0.723^*	1
	GSH	-0.664^*	0.576^*	0.832^*	0.165	0.853^*	0.597^*	0.927^*	1

eISSN:: 1848-6312
Języki:: Angielski, Slovenian

Częstotliwość wydawania:: 4 razy w roku
Dziedziny czasopisma:: Medicine, Basic Medical Science, other

Kanał RSS czasopisma

Pro-oxidant potency of clothianidin in rainbow trout

Article Category: Original article

Data publikacji: 28 cze 2021

Zakres stron: 164 - 172

Otrzymano: 01 sty 2021

Przyjęty: 01 maj 2021

DOI: https://doi.org/10.2478/aiht-2021-72-3522

© 2021 Tarek Fakhereddin and Demet Doğan, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

Effects of environmental clothianidin exposure on protein (mg/mL), GSH (nmol/mg protein), protein carbonyl (ng/mg protein), and MDA (nmol/mg protein) levels in heart and spleen tissues of rainbow trout

Heart and spleen somatic indices in rainbow trout by weeks of exposure to clothianidin

Pearson correlation coefficients for heart and spleen tissue biomarkers in rainbow trout following clothianidin exposure