<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Introduction</h3>
<p>Due to the severe side effects of non-steroidal anti-inflammatory drugs, there is an urgent need to find new natural or synthetic chemicals that could act as selective inhibitors of cyclooxygenase-2 (COX-2). The most valuable source of new bioactive compounds and potential drugs are plants. The present study aimed at the investigation of Levidor<sup>TM</sup>, a patented composition based on oil from the seeds of <italic>Nigella sativa</italic> and extract from the root of <italic>Angelica archangelica,</italic> regarding its potential anti-inflammatory effects on the molecular level.</p>
</sec>
<sec><h3>Materials and Methods</h3>
<p>Studies were carried out with the use of murine macrophages (RAW 264.7), human monocytes (U937), and rat pheochromocytoma (PC-12) cell lines. The cytotoxicity of the Levidor<sup>TM</sup> extract was evaluated by MTT assay. The concentration of pro-inflammatory cytokines (IL-6, TNF-<italic>α</italic>) was evaluated by ELISA. Moreover, the analysis of <italic>COX-1</italic> and <italic>COX-2</italic> mRNA expression levels by RT-PCR, as well as protein level by Western blot and immunofluorescent staining, were assessed.</p>
</sec>
<sec><h3>Results</h3>
<p>The Levidor<sup>TM</sup> extract did not impact cell growth and showed anti-inflammatory effect in the present study. It was able to decrease the level of IL-6, TNF-<italic>α</italic> and suppress transcription of COX-2, whereas it had no effect on COX-1.</p>
</sec>
<sec><h3>Conclusions</h3>
<p>Obtained results showed that Levidor<sup>TM</sup> exhibits strong anti-inflammatory properties and may provide a significant alternative to traditionally used medicines in acute pain and chronic inflammation.</p>
</sec>
</abstract>

Introduction
Due to the severe side effects of non-steroidal anti-inflammatory drugs, there is an urgent need to find new natural or synthetic chemicals that could act as selective inhibitors of cyclooxygenase-2 (COX-2). The most valuable source of new bioactive compounds and potential drugs are plants. The present study aimed at the investigation of LevidorTM, a patented composition based on oil from the seeds of Nigella sativa and extract from the root of Angelica archangelica, regarding its potential anti-inflammatory effects on the molecular level.

Materials and Methods
Studies were carried out with the use of murine macrophages (RAW 264.7), human monocytes (U937), and rat pheochromocytoma (PC-12) cell lines. The cytotoxicity of the LevidorTM extract was evaluated by MTT assay. The concentration of pro-inflammatory cytokines (IL-6, TNF-α) was evaluated by ELISA. Moreover, the analysis of COX-1 and COX-2 mRNA expression levels by RT-PCR, as well as protein level by Western blot and immunofluorescent staining, were assessed.

Results
The LevidorTM extract did not impact cell growth and showed anti-inflammatory effect in the present study. It was able to decrease the level of IL-6, TNF-α and suppress transcription of COX-2, whereas it had no effect on COX-1.

Conclusions
Obtained results showed that LevidorTM exhibits strong anti-inflammatory properties and may provide a significant alternative to traditionally used medicines in acute pain and chronic inflammation.

Anti-inflammatory activity of novel natural plant extracts composition—Levidor<sup>TM</sup>

Postępy Higieny i Medycyny Doświadczalnej

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Anti-inflammatory activity of novel natural plant extracts composition—Levidor

Introduction
Due to the severe side effects of non-steroidal anti-inflammatory drugs, there is an urgent need to find new natural or synthetic chemicals that...

Anti-inflammatory activity of novel natural plant extracts composition—Levidor^TM

Article Category: Original Study

Data publikacji: 22 maj 2023

Zakres stron: 49 - 58

Otrzymano: 14 paź 2022

Przyjęty: 21 mar 2023

DOI: https://doi.org/10.2478/ahem-2023-0008

Słowa kluczowe
phytochemicals, plant-based product, anti-inflammatory, cyclooxygenases

© 2023 Monika Kuczyńska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Anti-inflammatory activity of novel natural plant extracts composition—LevidorTM

Article Category: Original Study

Data publikacji: 22 maj 2023

Zakres stron: 49 - 58

Otrzymano: 14 paź 2022

Przyjęty: 21 mar 2023

DOI: https://doi.org/10.2478/ahem-2023-0008

Słowa kluczowephytochemicals, plant-based product, anti-inflammatory, cyclooxygenases

© 2023 Monika Kuczyńska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Anti-inflammatory activity of novel natural plant extracts composition—Levidor^TM

Słowa kluczowe
phytochemicals, plant-based product, anti-inflammatory, cyclooxygenases