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Influence of oxygen availability on expression of glutaminolysis genes in human colon cancer cells

Dagmara Otto-Ślusarczyk
Dagmara Otto-Ślusarczyk
, 
Wojciech Graboń
Wojciech Graboń
, 
Magdalena Mielczarek-Puta
Magdalena Mielczarek-Puta
, 
Alicja Chrzanowska
Alicja Chrzanowska
 oraz 
Anna Barańczyk-Kuźma
Anna Barańczyk-Kuźma
   | 20 gru 2021
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Article Category: Review
Data publikacji: 20 gru 2021
Zakres stron: 923 - 932
Otrzymano: 09 lis 2020
Przyjęty: 29 lip 2021
DOI: https://doi.org/10.2478/ahem-2021-0032
Słowa kluczowe
© 2021 Otto-Ślusarczyk., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Introduction

Glutaminolysis, beside glycolysis, is a key metabolic pathway of a cancer cell that provides energy and substrates for the synthesis of nucleic acids, proteins, and lipids. The pathway is mediated by both mitochondrial and cytosolic enzymes. Neither expression of glutaminolysis enzymes in colon cancer cells nor the influence of various oxygen concentrations on their expression has been studied so far.

Objectives

The aim of the study was to determine and compare the mRNA expression of enzymes involved in glutaminolysis at various oxygen levels in human primary (SW480) and metastatic (SW620) colon cancer cells cultured in 1% O2 (hypoxia), 10% O2 (tissue normoxia), 21% O2 (atmospheric normoxia).

Methods

Cell viability was determined by Trypan Blue exclusion (TB) and Thiazolyl Blue Tetrazolium Bromide (MTT). The expression of HIF1α, GLUT1, GLS1, AST1, AST2, ACL, PC and GC1, GC2 at mRNA levelwas determined by RT-qPCR. Results. Correlation between increasing oxygen concentration and cell count was not observed. In both cell lines the number of viable cells was the lowest at 10% oxygen. The enzyme profile and expression of proteins involved in glutaminolysis varied depending on oxygen pressure and type of cell lines. In summary, our findings suggest differences in metabolic adaptation to oxygen availability in vivo between primary and metastatic colon cancer cells.

eISSN:
1732-2693
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Life Sciences, Molecular Biology, Microbiology and Virology, Medicine, Basic Medical Science, Immunology
Kanał RSS czasopisma