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Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method

P. Chalova
P. Chalova
, 
D. Salaskova
D. Salaskova
, 
F. Csicsay
F. Csicsay
, 
J. Galba
J. Galba
, 
A. Kovac
A. Kovac
 oraz 
J. Piestansky
J. Piestansky
   | 31 sie 2023
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Article Category: Special Issue Article
Data publikacji: 31 sie 2023
Zakres stron: 67 - 74
Otrzymano: 30 maj 2023
Przyjęty: 23 cze 2023
DOI: https://doi.org/10.2478/afpuc-2023-0010
Słowa kluczowe
© 2023 P. Chalova et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

The biosynthesis of taurine from cysteine (a) and illustrative scheme of derivatization process of taurine using AccQ•Tag Ultra reagent (b).
The biosynthesis of taurine from cysteine (a) and illustrative scheme of derivatization process of taurine using AccQ•Tag Ultra reagent (b).

Figure 2.

Illustrative chromatograms of taurine and the IS obtained from the analysis of four energy drinks: a) Red Bull, b) Hell, c) Tiger, d) Maxx. For separation and detection conditions, see Section 2.1.
Illustrative chromatograms of taurine and the IS obtained from the analysis of four energy drinks: a) Red Bull, b) Hell, c) Tiger, d) Maxx. For separation and detection conditions, see Section 2.1.

UHPLC gradient program for the separation of taurine.

Time (min) Flow (mL/min) Mobile phase A (%) Mobile phase B (%)
Initial 0.5 99 1
0.7 0.5 99 1
1.3 0.5 87 13
3.7 0.5 85 15
7 0.5 60 40
8 0.5 5 95
9 0.5 5 95
9.8 0.5 99 1
10.5 0.5 99 1

Recovery of taurine tested at three concentration levels (10, 100, and 500 μmol/L).

Taurine
Added (μmol/L) 10 100 500
Found (μmol/L) 10.80 91.50 532.70
RSD (%), n = 5 0.8 2.7 0.6
Recovery (%) 108.0 91.5 106.5

Evaluation of accuracy parameters of taurine tested at three concentration levels (10, 100, and 500 μmol/L).

Nominal (μmol/L) Found (μmol/L) RSD (%) RE (%)
Intra-day, n = 5 10 10.92 2.62 9.2
100 90.99 0.10 −9.0
500 533.94 0.72 6.8
Inter-day, n = 10 10 10.95 3.95 9.5
100 92.31 0.57 −7.7
500 535.30 1.41 7.1

Determined concentration of taurine in four tested commercial available energy drinks.

Beverage Parameters
Found ± SD (mg/mL) RSD (%), n = 3 Declared (mg/mL) % of declared content
Red Bull 2.80 ± 0.07 2.6 4 70.0
Hell 3.77 ± 0.05 1.4 4 94.5
Tiger 3.78 ± 0.01 0.1 4 91.8
Maxx 3.67 ± 0.03 0.9 4 94.3

Overview of several selected operation and validation parameters of the UHPLC-MS method.

Taurine
tR (min) 1.96
a (counts) 0.00642644
RSDa (%), n = 6 9.7
b (counts × μmol−1 × L) 0.00801683
RSDb (%), n = 6 4.8
r2 0.9982
Linear range (μmol/L) 10–500
LOD (μmol/L) 4.29
LOQ (μmol/L) 10
N 93,340
eISSN:
2453-6725
Język:
Angielski
Częstotliwość wydawania:
2 razy w roku
Dziedziny czasopisma:
Pharmacy, other
Kanał RSS czasopisma