Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2
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18 cze 2022
O artykule
Kategoria artykułu: Short communication
Data publikacji: 18 cze 2022
Zakres stron: 87 - 89
Otrzymano: 21 kwi 2022
Przyjęty: 10 maj 2022
DOI: https://doi.org/10.2478/afpuc-2022-0007
Słowa kluczowe
© 2022 Mykhailo Huniadi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
SAP and EAT-2 are intracellular adaptor proteins that link SLAMF receptors to downstream intracellular signalling pathways. Their expression and functions vary among different types of hematopoietic cells. The roles of SAP and EAT-2 in haematological malignancies are poorly understood. Here, we attempted to optimise a method for the detection of intracellular SAP and EAT-2 by flow cytometry. We found that using PerFix EXPOSE kit for cell fixation and permeabilization ensures optimal results in terms of staining intensity and signal-to-noise ratio.