<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The oviduct play a crucial role in reproductive process, through facilitating successful embryo growth and conception. Oviduct activity is orchestrated by various factors, depending on cyclic dynamics, which crucially affect the success of reproductive function. The morphological modifications of oviducts in response to the female reproductive cycle are well established. However, detailed characterization at the molecular level is still needed. The present study, employed primary <italic>in vitro</italic> cell cultures and high-throughput transcriptome analysis via an Affymetrix microarray approach, described nucleotide, ribonucleotide and ribonucleoside binding patterns at a molecular level in oviduct epithelial cells (OECs). 222 genes were targeted belonging to four gene ontology biological process terms (GO BP): “adenyl nucleotide binding”, “adenyl ribonucleotide binding”, “ribonucleotide binding”, “ribonucleoside binding”, which showed the greatest variability in the level of mRNA expression during of long-term cultivation. In this group of genes, special attention was paid to those showing the greatest variability in relation to the reference measurement, including <italic>OASL</italic>, <italic>PIM1</italic>, <italic>ACTA2</italic> and <italic>ABCA1</italic>.</p><p><bold>Running title</bold>: Oviductal nucleotide and nucleoside binding patterns</p></abstract>

The oviduct play a crucial role in reproductive process, through facilitating successful embryo growth and conception. Oviduct activity is orchestrated by various factors, depending on cyclic dynamics, which crucially affect the success of reproductive function. The morphological modifications of oviducts in response to the female reproductive cycle are well established. However, detailed characterization at the molecular level is still needed. The present study, employed primary in vitro cell cultures and high-throughput transcriptome analysis via an Affymetrix microarray approach, described nucleotide, ribonucleotide and ribonucleoside binding patterns at a molecular level in oviduct epithelial cells (OECs). 222 genes were targeted belonging to four gene ontology biological process terms (GO BP): “adenyl nucleotide binding”, “adenyl ribonucleotide binding”, “ribonucleotide binding”, “ribonucleoside binding”, which showed the greatest variability in the level of mRNA expression during of long-term cultivation. In this group of genes, special attention was paid to those showing the greatest variability in relation to the reference measurement, including OASL, PIM1, ACTA2 and ABCA1.Running title: Oviductal nucleotide and nucleoside binding patterns

Nucleotide, ribonucleotide and ribonucleoside binding belongs to differentially expressed genes in porcine epithelial oviductal cells during longterm primary cultivation

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The oviduct play a crucial role in reproductive process, through facilitating successful embryo growth and conception. Oviduct activity is orchestrated by...

Nucleotide, ribonucleotide and ribonucleoside binding belongs to differentially expressed genes in porcine epithelial oviductal cells during longterm primary cultivation

Data publikacji: 21 gru 2019

Zakres stron: 161 - 169

Otrzymano: 03 lis 2019

Przyjęty: 08 gru 2019

DOI: https://doi.org/10.2478/acb-2019-0022

Słowa kluczowe
oviductal epithelial cells, culture, nucleotide, nucleoside

© 2019 Mariusz J. Nawrocki, Rafał Sibiak, Sandra Kałużna, Maciej Brązert, Piotr Celichowski, Leszek Pawelczyk, Lisa Moncrieff, Bartosz Kempisty, Paul Mozdziak, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Nucleotide, ribonucleotide and ribonucleoside binding belongs to differentially expressed genes in porcine epithelial oviductal cells during longterm primary cultivation

Data publikacji: 21 gru 2019

Zakres stron: 161 - 169

Otrzymano: 03 lis 2019

Przyjęty: 08 gru 2019

DOI: https://doi.org/10.2478/acb-2019-0022

Słowa kluczoweoviductal epithelial cells, culture, nucleotide, nucleoside

© 2019 Mariusz J. Nawrocki, Rafał Sibiak, Sandra Kałużna, Maciej Brązert, Piotr Celichowski, Leszek Pawelczyk, Lisa Moncrieff, Bartosz Kempisty, Paul Mozdziak, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
oviductal epithelial cells, culture, nucleotide, nucleoside