Otwarty dostęp

Treatment of cholangiocarcinoma by pGCsiRNA-vascular endothelial growth factor in vivo

Shenglin Lu
Shenglin Lu
 oraz 
Jun Li
Jun Li
   | 30 kwi 2024
Asian Biomedicine's Cover Image
O artykule
Poprzedni artykuł
Następny artykuł
Zacytuj
Article Category: Original article
Data publikacji: 30 kwi 2024
Zakres stron: 61 - 68
DOI: https://doi.org/10.2478/abm-2024-0009
Słowa kluczowe
© 2024 Shenglin Lu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1.

Sequencing results of pGCsiRNA-VEGF containing U6 promoter. The results showed that 6.3 kb linearized vectors and 63 bp oligonucleotide fragments were obtained. pGCsiRNA, VEGF, vascular endothelial growth factor.
Sequencing results of pGCsiRNA-VEGF containing U6 promoter. The results showed that 6.3 kb linearized vectors and 63 bp oligonucleotide fragments were obtained. pGCsiRNA, VEGF, vascular endothelial growth factor.

Figure 2.

Tumor growth curves. The tumor volume was smaller in the pGCsiRNA-VEGF group than that in the mock group and the si-scramble group on Day 27 of treatment. VEGF, vascular endothelial growth factor.
Tumor growth curves. The tumor volume was smaller in the pGCsiRNA-VEGF group than that in the mock group and the si-scramble group on Day 27 of treatment. VEGF, vascular endothelial growth factor.

Figure 3.

Tumor weights. On Day 28 of treatment, the tumor weight was smaller in the pGCsiRNA-VEGF group than that in the mock group and the si-scramble group. VEGF, vascular endothelial growth factor.
Tumor weights. On Day 28 of treatment, the tumor weight was smaller in the pGCsiRNA-VEGF group than that in the mock group and the si-scramble group. VEGF, vascular endothelial growth factor.

Figure 4.

Tumor volumes. Tumor growth was inhibited in nude mice. VEGF, vascular endothelial growth factor.
Tumor volumes. Tumor growth was inhibited in nude mice. VEGF, vascular endothelial growth factor.

Figure 5.

Protein expressions of VEGF, MMP2 and MMP9. The protein expressions of VEGF, MMP2, and MMP9 declined in pGCsiRNA-VEGF group compared with those in the mock and si-scramble groups. MMP, matrix metalloproteinase; VEGF, vascular endothelial growth factor.
Protein expressions of VEGF, MMP2 and MMP9. The protein expressions of VEGF, MMP2, and MMP9 declined in pGCsiRNA-VEGF group compared with those in the mock and si-scramble groups. MMP, matrix metalloproteinase; VEGF, vascular endothelial growth factor.

Figure 6.

Changes in mRNA expressions of VEGF, MMP2, and MMP9 in tumor tissues. The mRNA expressions of VEGF, MMP2, and MMP9 were significantly lower in the pGCsiRNA-VEGF group than those in the mock group and the si-scramble group. MMP, matrix metalloproteinase; VEGF, vascular endothelial growth factor.
Changes in mRNA expressions of VEGF, MMP2, and MMP9 in tumor tissues. The mRNA expressions of VEGF, MMP2, and MMP9 were significantly lower in the pGCsiRNA-VEGF group than those in the mock group and the si-scramble group. MMP, matrix metalloproteinase; VEGF, vascular endothelial growth factor.

Figure 7.

Morphological changes of tumor tissues. There was obvious apoptosis of tumor tissues, a large number of cells died, the tissue structures mostly disappeared, karyopyknosis was found, a large number of apoptotic cells were dispersed around the tissues, and the cells lost their normal morphology in pGCsiRNA-VEGF group compared to those in the mock and si-scramble groups. VEGF, vascular endothelial growth factor.
Morphological changes of tumor tissues. There was obvious apoptosis of tumor tissues, a large number of cells died, the tissue structures mostly disappeared, karyopyknosis was found, a large number of apoptotic cells were dispersed around the tissues, and the cells lost their normal morphology in pGCsiRNA-VEGF group compared to those in the mock and si-scramble groups. VEGF, vascular endothelial growth factor.

Figure 8.

VEGF expressions of the different groups. The protein expression of VEGF was significantly suppressed in the pGCsiRNA-VEGF group compared with that in the mock group and the si-scramble group. VEGF, vascular endothelial growth factor.
VEGF expressions of the different groups. The protein expression of VEGF was significantly suppressed in the pGCsiRNA-VEGF group compared with that in the mock group and the si-scramble group. VEGF, vascular endothelial growth factor.
eISSN:
1875-855X
Język:
Angielski
Częstotliwość wydawania:
6 razy w roku
Dziedziny czasopisma:
Medicine, Assistive Professions, Nursing, Basic Medical Science, other, Clinical Medicine
Kanał RSS czasopisma