Mycosynthesis of Size-Controlled Silver Nanoparticles through Optimization of Process Variables by Response Surface Methodology

ASMA SHAHZAD; MEHWISH IQTEDAR; HAMID SAEED; SYED ZAJIF HUSSAIN; ASMA CHAUDHARY; ROHEENA ABDULLAH; AFSHAN KALEEM

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Mycosynthesis of Size-Controlled Silver Nanoparticles through Optimization of Process Variables by Response Surface Methodology

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| 27 mar 2019

Polish Journal of Microbiology

Tom 68 (2019): Zeszyt 1 (March 2019)

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Article Category: original-paper

Data publikacji: 27 mar 2019

Zakres stron: 35 - 42

DOI: https://doi.org/10.21307/pjm-2019-004

Słowa kluczowe
and AgNPs, Central Composite Design (CCD) for AgNPs synthesis, green synthesis of AgNPs, nitrate reductase activity for AgNPs synthesis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of AgNPs obtained WO.A. UV-Vis spectrophotometer analysis representing a peak of AgNPs at 452 nm and showing brown coloured nanoparticles. B. Zeta sizer (DLS) analysis representing peak at 356nm nm. C. Triangular shaped AgNPs revealed by AFM. D. Fourier transform infrared (FT-IR) analysis of AgNPs.

Characterization of AgNPs obtained OS.A. UV-Vis spectrophotometer analysis representing peak of AgNPs at 420 nm and showing medium brown coloured nanoparticles. B. Zeta sizer (DLS) analysis representing peak at 33.23 nm. C. Zeta potential analysis by DLS. D. Spherical shaped AgNPs revealed by AFM. E. Fourier transform infrared (FT-IR) analysis of AgNPs indicating presence of functional groups.

A. Colour change observed during the optimization experiments (20 runs). B. Estimation of nitrate reductase activity (nmol/h/ml) and reduction in the size of AgNPs during different RSM experiments.

3D surface plots analysis. A. Effect of substrate concentration and metal salt concentration. B. Effect of metal salt concentration and substrate concentration. C. Effect of substrate concentration and wet weight of biomass.

Central composite design (CCD) matrix of three independent variables for AgNPs biosynthesis in codes with experimental results.

Runs	Factor A: Substrate concentration (mM)	Factor B: NaCl concentration (%)	Factor C: Wet weight of biomass (g)	Response: Size of AgNPs (nm)
1	1	10	7	33.65
2	3	20	7	127.20
3	2	15	8.5	68.23
4	1	20	10	33.65
5	3	1	10	76.22
6	1	20	7	33.65
7	2	15	8.5	68.20
8	2	15	8.5	68.30
9	2	23.41	8.5	89.37
10	1	10	10	33.65
11	3	20	10	70.48
12	3.68	15	8.5	156.2
13	2	15	11.02	33.65
14	2	15	8.5	33.55
15	2	15	8.5	33.65
16	0.32	15	8.5	126.30
17	2	15	5.98	33.23
18	2	6.59	8.5	33.45
19	3	10	7	127.10
20	2	15	8.5	90.06

Analysis of Variance (ANOVA) of the Fitted Quadratic Model and regression analysis for optimization of AgNPs biosynthesis.

Source	Sum of Squares	Df	Mean Square	F-Value	p-value	Prob > F
Model	23241.50	9	2582.39	3.91	0.0224	SignificantSignificant
A – Substrate concentration	7343.55	1	7343.55	11.11	0.0076
B – NaCl concentration	572.28	1	572.28	0.87	0.3740
C – Wet weight of biomass	836.67	1	836.67	1.27	0.2868
AB	3.98	1	3.98	0.006017	0.9397
AC	1447.22	1	1447.22	2.19	0.1697
BC	4.26	1	4.26	0.006451	0.9376
A²	9658.74	1	9658.74	14.62	0.0034	Significant
B²	78.85	1	78.85	0.12	0.7369
C²	2154.83	1	2154.83	3.26	0.1011
Residual	6608.52	10	660.85
Lack of Fit	4113.14	5	822.63	1.65	0.2984	Not significant
Pure Error	2495.38	5	499.08
Core Total	29850.02	19
Std. dev	C.V	R-Squared	Adj R-Squared	Pred R-Squared	Adeq Precision
25.71	37.53	0.7786	0.5794	0.1664	8.801

eISSN:: 2544-4646
Język:: Angielski

Częstotliwość wydawania:: 4 razy w roku
Dziedziny czasopisma:: Life Sciences, Microbiology and Virology

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Mycosynthesis of Size-Controlled Silver Nanoparticles through Optimization of Process Variables by Response Surface Methodology

Article Category: original-paper

Data publikacji: 27 mar 2019

Zakres stron: 35 - 42

DOI: https://doi.org/10.21307/pjm-2019-004

Słowa kluczowe
and AgNPs, Central Composite Design (CCD) for AgNPs synthesis, green synthesis of AgNPs, nitrate reductase activity for AgNPs synthesis

© 2019 Maryam Labibzadeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Central composite design (CCD) matrix of three independent variables for AgNPs biosynthesis in codes with experimental results.

Analysis of Variance (ANOVA) of the Fitted Quadratic Model and regression analysis for optimization of AgNPs biosynthesis.

Mycosynthesis of Size-Controlled Silver Nanoparticles through Optimization of Process Variables by Response Surface Methodology

Article Category: original-paper

Data publikacji: 27 mar 2019

Zakres stron: 35 - 42

DOI: https://doi.org/10.21307/pjm-2019-004

Słowa kluczoweand AgNPs, Central Composite Design (CCD) for AgNPs synthesis, green synthesis of AgNPs, nitrate reductase activity for AgNPs synthesis

© 2019 Maryam Labibzadeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Central composite design (CCD) matrix of three independent variables for AgNPs biosynthesis in codes with experimental results.

Analysis of Variance (ANOVA) of the Fitted Quadratic Model and regression analysis for optimization of AgNPs biosynthesis.

Słowa kluczowe
and AgNPs, Central Composite Design (CCD) for AgNPs synthesis, green synthesis of AgNPs, nitrate reductase activity for AgNPs synthesis