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Epilithic Biofilms in Lake Baikal: Screening and Diversity of PKS and NRPS Genes in the Genomes of Heterotrophic Bacteria

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10 gru 2018

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Aleti G, Sessitsch, A, Brader G. 2015. Genome mining: prediction of lipopeptides and polyketides from Bacillus and related Firmicutes. Comput Struct Biotechnol J. 13:192–203.AletiGSessitschABraderG2015Genome mining: prediction of lipopeptides and polyketides from Bacillus and related FirmicutesComput Struct Biotechnol J1319220310.1016/j.csbj.2015.03.003Search in Google Scholar

Banskota AH, Mcalpine JB, Sørensen D, Ibrahim A, Aouidate M, Piraee M, Alarco AM, Farnet CM, Zazopoulos E. 2006. Genomic analyses lead to novel secondary metabolites. Part 3. ECO-0501, a novel antibacterial of a new class. J Antibiot. 9:533–542.BanskotaAHMcalpineJBSørensenDIbrahimAAouidateMPiraeeMAlarcoAMFarnetCMZazopoulosE2006Genomic analyses lead to novel secondary metabolites. Part 3. ECO-0501, a novel antibacterial of a new classJ Antibiot953354210.1038/ja.2006.74Search in Google Scholar

Bartrons M, Catalan J, Casamayor EO. 2012. High bacterial diversity in epilithic biofilms of oligotrophic mountain lakes. Microb Ecol. 4:860–869.BartronsMCatalanJCasamayorEO2012High bacterial diversity in epilithic biofilms of oligotrophic mountain lakesMicrob Ecol486086910.1007/s00248-012-0072-4Search in Google Scholar

Batool M, Khalid MH, Hassan MN, Hafeez FY. 2011. Homology modeling of an antifungal metabolite plipastatin synthase from the Bacillus subtilis 168. Bioinformation 7(8):384–387.BatoolMKhalidMHHassanMNHafeezFY2011Homology modeling of an antifungal metabolite plipastatin synthase from the Bacillus subtilis 168Bioinformation7(8):38438710.6026/97320630007384Search in Google Scholar

Berge O, Guinebretie’re MH, Achouak W, Normand P, Heulin T. 2002. Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Micr. 52:607–616.BergeOGuinebretie’reMHAchouakWNormandPHeulinT2002Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and foodInt J Syst Evol Micr5260761610.1099/00207713-52-2-607Search in Google Scholar

Bie X, Lu Z, Lu F. 2009. Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID. J Microbiol Methods. 79:272–278.BieXLuZLuF2009Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CIDJ Microbiol Methods7927227810.1016/j.mimet.2009.09.013Search in Google Scholar

Brosius J, Dull TJ, Sleeter DD, Noller HF. 1981. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol. 148:107–127.BrosiusJDullTJSleeterDDNollerHF1981Gene organization and primary structure of a ribosomal RNA operon from Escherichia coliJ Mol Biol14810712710.1016/0022-2836(81)90508-8Search in Google Scholar

Ciesiołka J, Jeżowska-Bojczuk M, Wrzesiński J, Stokowa-Sołtys K, Nagaj J, Kasprowicz A, Błaszczyk L, Szczepanik W. 2014. Antibiotic bacitracin induces hydrolytic degradation of nucleic acids. Biochim Biophys Acta. 1840(6):1782–1789.CiesiołkaJJeżowska-BojczukMWrzesińskiJStokowa-SołtysKNagajJKasprowiczABłaszczykLSzczepanikW2014Antibiotic bacitracin induces hydrolytic degradation of nucleic acidsBiochim Biophys Acta1840(6):1782178910.1016/j.bbagen.2014.01.03424508122Search in Google Scholar

Chen XH, Koumoutsi A, Scholz R, Borriss R. 2009. More than anticipated – production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42. J Mol Microbiol Biotechnol. 16(1–2):14–24.ChenXHKoumoutsiAScholzRBorrissR2009More than anticipated – production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42J Mol Microbiol Biotechnol16(1–2):142410.1159/00014289118957859Search in Google Scholar

Choi SK, Park SY, Kim R, Lee CH, Kim JF, Park SH. 2008. Identification and functional analysis of the fusaricidin biosynthetic gene of Paenibacillus polymyxa E681. Biochem Biophys Res Commun. 365(1):89–95.ChoiSKParkSYKimRLeeCHKimJFParkSH2008Identification and functional analysis of the fusaricidin biosynthetic gene of Paenibacillus polymyxa E681Biochem Biophys Res Commun365(1):899510.1016/j.bbrc.2007.10.14717980146Search in Google Scholar

Cochrane SA, Lohans CT, van Belkum MJ, Bels MA, Vederas JC. 2015. Studies on tridecaptin B(1), a lipopeptide with activity against multidrug resistant Gram-negative bacteria. Org Biomol Chem. 13:6073–6081.CochraneSALohansCTvan BelkumMJBelsMAVederasJC2015Studies on tridecaptin B(1), a lipopeptide with activity against multidrug resistant Gram-negative bacteriaOrg Biomol Chem136073608110.1039/C5OB00780A25959079Search in Google Scholar

Cosmina P, Rodriguez F, de Ferra F, Grandi G, Perego M, Venema G, van Sinderen D. 1993. Sequence and analysis of the genetic locus responsible for surfactin synthesis in Bacillus subtilis. Mol Microbiol. 8(5):821–831.CosminaPRodriguezFde FerraFGrandiGPeregoMVenemaGvan SinderenD1993Sequence and analysis of the genetic locus responsible for surfactin synthesis in Bacillus subtilisMol Microbiol8(5):82183110.1111/j.1365-2958.1993.tb01629.x8355609Search in Google Scholar

Costerton JW, Cheng KJ, Geesey GG, Ladd TI, Nickel JC, Dasgupta M, Marrie TJ. 1987. Bacterial biofilms in nature and disease. Annu Rev Microbiol. 41:435–464.CostertonJWChengKJGeeseyGGLaddTINickelJCDasguptaMMarrieTJ1987Bacterial biofilms in nature and diseaseAnnu Rev Microbiol4143546410.1146/annurev.mi.41.100187.0022513318676Search in Google Scholar

Dayu Y, Fuchao X, Jia Z, Jixun Z. 2012. Type III Polyketide Synthases in Natural Product Biosynthesis. IUBMB Life. 64(4):285–295.DayuYFuchaoXJiaZJixunZ2012Type III Polyketide Synthases in Natural Product BiosynthesisIUBMB Life64(4):28529510.1002/iub.100522362498Search in Google Scholar

Donlan RM, Costerton JW. 2002. Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms. Clin Microbiol Rev. 15(2):167–193.DonlanRMCostertonJW2002Biofilms: Survival Mechanisms of Clinically Relevant MicroorganismsClin Microbiol Rev15(2):16719310.1128/CMR.15.2.167-193.200211806811932229Search in Google Scholar

Ehrenreich I, Waterbury J, Webb E. 2005. Distribution and diversity of natural product genes in marine and freshwater cyanobacterial cultures and genomes. Appl Environ Microb. 71(11):7401–7413.EhrenreichIWaterburyJWebbE2005Distribution and diversity of natural product genes in marine and freshwater cyanobacterial cultures and genomesAppl Environ Microb71(11):7401741310.1128/AEM.71.11.7401-7413.2005128767216269782Search in Google Scholar

Fickers P. 2012. Antibiotic compounds from Bacillus: Why are they so amazing? Am J Biochem Biotechnol. 1:40–46.FickersP2012Antibiotic compounds from Bacillus: Why are they so amazing?Am J Biochem Biotechnol14046Search in Google Scholar

Jeong H, Choi SK, Park SY, Kim SH, Parka SH. 2012. Draft genome sequence of Paenibacillus peoriae strain KCTC 3763T. J Bacteriol. 194:1237–1238.JeongHChoiSKParkSYKimSHParkaSH2012Draft genome sequence of Paenibacillus peoriae strain KCTC 3763TJ Bacteriol1941237123810.1128/JB.06577-11329482022328743Search in Google Scholar

Johnson BA, Anker H, Meleney FL. 1945. Bacitracin: a new antibiotic produced by a member of the B. subtilis group. Science. 102(2650):376–377.JohnsonBAAnkerHMeleneyFL1945Bacitracin: a new antibiotic produced by a member of the B. subtilis groupScience102(2650):37637710.1126/science.102.2650.37617770204Search in Google Scholar

Kaluzhnaya OV, Kulakova NV, Itskovich BV. 2012. Diversity of polyketide synthase genes (PKS) in metagenomic community of the freshwater sponge Lubomirskia baicalensi. Mol Biol (Moscow). 6:887–893.KaluzhnayaOVKulakovaNVItskovichBV2012Diversity of polyketide synthase genes (PKS) in metagenomic community of the freshwater sponge Lubomirskia baicalensiMol Biol (Moscow)688789310.1134/S002689331206009XSearch in Google Scholar

Kalyuzhnaya OV, Lipko IA, Itskovich VB, Kalyuzhnaya OV, Parfenova VV. 2013. PCR-screening of bacteria isolated from freshwater sponge Lubomirskia baicalensis for detection of genes of secondary metabolites. Water: Chemistry and Ecology. 7:70−74.KalyuzhnayaOVLipkoIAItskovichVBKalyuzhnayaOVParfenovaVV2013PCR-screening of bacteria isolated from freshwater sponge Lubomirskia baicalensis for detection of genes of secondary metabolitesWater: Chemistry and Ecology77074Search in Google Scholar

Kaluzhnaya OV, Itskovich VB. 2016. Distinctive features of the microbial diversity and the polyketide synthase genes spectrum in the community of the endemic Baikal sponge Swartschewskia papyracea. Russ J Genet (Moscow). 1: 38–48.KaluzhnayaOVItskovichVB2016Distinctive features of the microbial diversity and the polyketide synthase genes spectrum in the community of the endemic Baikal sponge Swartschewskia papyraceaRuss J Genet (Moscow)1384810.1134/S1022795416010099Search in Google Scholar

Karala AR, Ruddock LW. 2010. Bacitracin is not a specific inhibitor of protein disulfide isomerase. FEBS Journal. 277(11):2454–2462.KaralaARRuddockLW2010Bacitracin is not a specific inhibitor of protein disulfide isomeraseFEBS Journal277(11):2454246210.1111/j.1742-4658.2010.07660.x20477872Search in Google Scholar

Kim PI, Ryu J, Kim YH, Chi YT. 2010. Production of biosurfactant lipopeptides iturin A, fengycin, and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J Microbiol Biotechnol. 20(1):138–145.KimPIRyuJKimYHChiYT2010Production of biosurfactant lipopeptides iturin A, fengycin, and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioidesJ Microbiol Biotechnol20(1):13814510.4014/jmb.0905.05007Search in Google Scholar

Li J, Beatty PK, Shah S, Jensen SE. 2007. Use of PCR-targeted mutagenesis to disrupt production of fusaricidin-type antifungal antibiotics in Paenibacillus polymyxa. Appl Environ Microbiol. 73(11):3480–3489.LiJBeattyPKShahSJensenSE2007Use of PCR-targeted mutagenesis to disrupt production of fusaricidin-type antifungal antibiotics in Paenibacillus polymyxaAppl Environ Microbiol73(11):3480348910.1128/AEM.02662-06193266817400768Search in Google Scholar

Li XY, Mao ZC, Wang YH, Wu YX, He YQ, Long CL. 2012. ESI LC-MS and MS/MS characterization of antifungal cyclic lipopeptides produced by Bacillus subtilis XF-1. J Mol Microbiol Biotechnol. 22(2):83–93.LiXYMaoZCWangYHWuYXHeYQLongCL2012ESI LC-MS and MS/MS characterization of antifungal cyclic lipopeptides produced by Bacillus subtilis XF-1J Mol Microbiol Biotechnol22(2):839310.1159/00033853022614917Search in Google Scholar

Lipko IA (Terkina), Kaluzhnaya OV, Kravchenko OS, Parfenova VV. 2012. Identification of the polyketide synthase genes (PKS) in genome of the strain Pseudomonas fluorescens 28Bb-06 from the freshwater sponge Baikalospongia bacillifera. Mol Biol (Moscow). 4:677–679.LipkoIA (Terkina), KaluzhnayaOVKravchenkoOSParfenovaVV2012Identification of the polyketide synthase genes (PKS) in genome of the strain Pseudomonas fluorescens 28Bb-06 from the freshwater sponge Baikalospongia bacilliferaMol Biol (Moscow)4677679Search in Google Scholar

Liu WB, Yu WB, Gao SH, Ye BC. 2013. Genome sequence of Saccharopolyspora erythraea D, a hyperproducer of erythromycin. Genome Announc. 1:e00718–13.LiuWBYuWBGaoSHYeBC2013Genome sequence of Saccharopolyspora erythraea D, a hyperproducer of erythromycinGenome Announc1e00718–13Search in Google Scholar

Lorentz RH, Artico S, da Silveira AB, Einsfeld A, Corção G. 2006. Evaluation of antimicrobial activity in Paenibacillus spp. strains isolated from natural environment. Lett Appl Microbiol. 43(5):541−547.LorentzRHArticoSda SilveiraABEinsfeldACorçãoG2006Evaluation of antimicrobial activity in Paenibacillus spp. strains isolated from natural environmentLett Appl Microbiol43(5):54154710.1111/j.1472-765X.2006.01995.x17032229Search in Google Scholar

Meerak J, Yukphan P, Miyashita M, Sato H, Nakagawa Y, Tahara Y. 2008. Phylogeny of gamma-polyglutamic acid-producing Bacillus strains isolated from a fermented locust bean product manufactured in West Africa. J Gen Appl Microbiol. 54:159-166.MeerakJYukphanPMiyashitaMSatoHNakagawaYTaharaY2008Phylogeny of gamma-polyglutamic acid-producing Bacillus strains isolated from a fermented locust bean product manufactured in West AfricaJ Gen Appl Microbiol5415916610.2323/jgam.54.15918654037Search in Google Scholar

Mondol MA, Shin HJ, Islam MT. 2013. Diversity of secondary metabolites from marine Bacillus species: chemistry and biological activity. Mar Drugs. 11(8):2846−2872.MondolMAShinHJIslamMT2013Diversity of secondary metabolites from marine Bacillus species: chemistry and biological activityMar Drugs11(8):2846287210.3390/md11082846376686923941823Search in Google Scholar

Netrusov AI. 2005. Praktikum po mikrobiologii (Practical Course in Microbiology). Moscow (Russia): Akademiya.NetrusovAI2005Praktikum po mikrobiologii (Practical Course in Microbiology)Moscow (Russia)AkademiyaSearch in Google Scholar

Ongena M, Jacques P. 2008. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol. 16(3):115–125.OngenaMJacquesP2008Bacillus lipopeptides: versatile weapons for plant disease biocontrolTrends Microbiol16(3):11512510.1016/j.tim.2007.12.00918289856Search in Google Scholar

Palomo S, González I, Cruz M, Martín J, Tormo JR, Anderson M, Hill RT, Vicente F, Reyes F, Genilloud O. 2013. Sponge-derived Kocuria and Micrococcus spp. as sources of the new thiazolyl peptide antibiotic kocurin. Mar Drugs. 11:1071–1086.PalomoSGonzálezICruzMMartínJTormoJRAndersonMHillRTVicenteFReyesFGenilloudO2013Sponge-derived Kocuria and Micrococcus spp. as sources of the new thiazolyl peptide antibiotic kocurinMar Drugs111071108610.3390/md11041071370538923538871Search in Google Scholar

Parfenova VV, Mal’nik VV, Boiko SM, Sheveleva NG, Logacheva NF, Evstigneeva TD, Suturin AN, Timoshkin OA. 2008. Communities of hydrobionts developing at the water-rock interface in Lake Baikal. Russ J Ecol. 39 (3):198–204.ParfenovaVVMal’nikVVBoikoSMShevelevaNGLogachevaNFEvstigneevaTDSuturinANTimoshkinOA2008Communities of hydrobionts developing at the water-rock interface in Lake BaikalRuss J Ecol39(3):19820410.1134/S1067413608030089Search in Google Scholar

Parfenova VV, Gladkikh AS, Belykh OI. 2013. Comparative analysis of biodiversity in the planktonic and biofilm bacterial communities in Lake Baikal. Microbiology. 82(1):91–101.ParfenovaVVGladkikhASBelykhOI2013Comparative analysis of biodiversity in the planktonic and biofilm bacterial communities in Lake BaikalMicrobiology82(1):9110110.1134/S0026261713010128Search in Google Scholar

Peypoux F, Besson F, Michel G, Delcambe L. 1981. Structure of bacillomycin D, a new antibiotic of the iturin group. Eur J Biochem. 118:323–327.PeypouxFBessonFMichelGDelcambeL1981Structure of bacillomycin D, a new antibiotic of the iturin groupEur J Biochem11832332710.1111/j.1432-1033.1981.tb06405.xSearch in Google Scholar

Romaní AM, Sabater S. 2001. Structure and activity of rock and sand biofilms in a Mediterranean stream. Ecology. 82:3232–3245.RomaníAMSabaterS2001Structure and activity of rock and sand biofilms in a Mediterranean streamEcology823232324510.1890/0012-9658(2001)082[3232:SAAORA]2.0.CO;2Search in Google Scholar

Romaní AM, Guasch H, Dolors Balaguer M. 2016. Aquatic Biofilms. Ecology, water quality and wastewater treatment. Norfolk (UK): Caister Academic Press 230 p.RomaníAMGuaschHDolors BalaguerM2016Aquatic Biofilms. Ecology, water quality and wastewater treatmentNorfolk (UK)Caister Academic Press230Search in Google Scholar

Romero D, de Vicente A, Rakotoaly RH, Dufour SE, Veening JW, Arrebola E, Cazorla FM, Kuipers OP, Paquot M, Pérez-García A. 2007. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Mol Plant Microbe Interact. 20(4):430–440.RomeroDde VicenteARakotoalyRHDufourSEVeeningJWArrebolaECazorlaFMKuipersOPPaquotMPérez-GarcíaA2007The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fuscaMol Plant Microbe Interact20(4):43044010.1094/MPMI-20-4-0430Search in Google Scholar

Roongsawang N, Washio K, Morikawa M. 2010. Diversity of nonribosomal peptide synthetases involved in the biosynthesis of lipopeptide biosurfactants. Int J Mol Sci. 12(1):141–172.RoongsawangNWashioKMorikawaM2010Diversity of nonribosomal peptide synthetases involved in the biosynthesis of lipopeptide biosurfactantsInt J Mol Sci12(1):14117210.3390/ijms12010141Search in Google Scholar

Sponga F, Cavaletti L, Lazzarini A, Borghi A, Ciciliato I, Losi D, Marinelli F. 1999. Biodiversity and potentials of marine-derived microorganisms. J Biotechnol. 70(1):65−69.SpongaFCavalettiLLazzariniABorghiACiciliatoILosiDMarinelliF1999Biodiversity and potentials of marine-derived microorganismsJ Biotechnol70(1):656910.1016/S0168-1656(99)00059-0Search in Google Scholar

Staunton J, Wilkinson B. 2001. Combinatorial biosynthesis of polyketides and non-ribosomal peptides. Curr Opin Chem Biol. 5:159−164.StauntonJWilkinsonB2001Combinatorial biosynthesis of polyketides and non-ribosomal peptidesCurr Opin Chem Biol515916410.1016/S1367-5931(00)00185-XSearch in Google Scholar

Sukhanova EV, Zimens EA, Parfenova VV, Belykh OI. 2017. Diversity of polyketide synthase genes in the genomes of heterotrophic microorganisms isolated from epilithic biofilms of Lake Baikal. Moscow University Biological Sciences Bulletin. 72(4):211–217.SukhanovaEVZimensEAParfenovaVVBelykhOI2017Diversity of polyketide synthase genes in the genomes of heterotrophic microorganisms isolated from epilithic biofilms of Lake BaikalMoscow University Biological Sciences Bulletin72(4):21121710.3103/S0096392517040113Search in Google Scholar

Terkina IA, Parfenova VV, Ahn TS. 2006. Antagonistic activity of actinomyces of Lake Baikal. Prikl Biokhim Mikrobiol. 2: 95−199.TerkinaIAParfenovaVVAhnTS2006Antagonistic activity of actinomyces of Lake BaikalPrikl Biokhim Mikrobiol29519910.1134/S0003683806020104Search in Google Scholar

Timoshkin OA, Suturin AN, Maximova NV, et al. 2003. Rock preferences and microdistribution peculiarities of Porifera and Gastropoda in the shallow littoral zone of Lake Baikal (East Siberia) as evidenced by underwater macrophotograph analysis. Berliner Palaeobiologische Abhandlungen Berlin. 4:193–200.TimoshkinOASuturinANMaximovaNV2003Rock preferences and microdistribution peculiarities of Porifera and Gastropoda in the shallow littoral zone of Lake Baikal (East Siberia) as evidenced by underwater macrophotograph analysisBerliner Palaeobiologische Abhandlungen Berlin4193200Search in Google Scholar

Wu XC, Qian CD, Fang HH, Wen YP, Zhou JY, Zhan ZJ, Ding R, Li O, Gao H. 2011. Paenimacrolidin, a novel macrolide antibiotic from Paenibacillus sp. F6-B70 active against methicillin-resistant Staphylococcus aureus. Microb Biotechnol. 4:491–502.WuXCQianCDFangHHWenYPZhouJYZhanZJDingRLiOGaoH2011Paenimacrolidin, a novel macrolide antibiotic from Paenibacillus sp. F6-B70 active against methicillin-resistant Staphylococcus aureusMicrob Biotechnol449150210.1111/j.1751-7915.2010.00201.xSearch in Google Scholar

Zhang B, Dong C, Shang Q, Cong Y, Kong W, Li P. 2013. Purification and partial characterization of bacillomycin L produced by Bacillus amyloliquefaciens K103 from lemon. Appl Biochem Biotechnol. 171:2262–2272.ZhangBDongCShangQCongYKongWLiP2013Purification and partial characterization of bacillomycin L produced by Bacillus amyloliquefaciens K103 from lemonAppl Biochem Biotechnol1712262227210.1007/s12010-013-0424-724043450Search in Google Scholar

Zhang W, Zhang F, Li Z, Miao X, Meng Q, Zhang X. 2009. Investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from South China Sea sponges. Appl Microbiol. 107:567–575.ZhangWZhangFLiZMiaoXMengQZhangX2009Investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from South China Sea spongesAppl Microbiol10756757510.1111/j.1365-2672.2009.04241.x19302490Search in Google Scholar

Zhao X, Kuipers OP. 2016. Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species. BMC Genomics. 17:882.ZhaoXKuipersOP2016Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales speciesBMC Genomics1788210.1186/s12864-016-3224-y510033927821051Search in Google Scholar

Zimens EA, Sukhanova EV, Shtykova YR, Parfenova VV, Belkova NL. 2014. Antagonistic activity of heterotrophic microorganisms isolated from biofilms of hard substrates in littoral zone of Lake Baikal. ISU Proceedings Series ‘Biology. Ecology’. 7:91-98.ZimensEASukhanovaEVShtykovaYRParfenovaVVBelkovaNL2014Antagonistic activity of heterotrophic microorganisms isolated from biofilms of hard substrates in littoral zone of Lake BaikalISU Proceedings Series ‘Biology. Ecology’79198Search in Google Scholar

Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Mikrobiologia i wirusologia