Otwarty dostęp

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

DA CHENG HAO
DA CHENG HAO
, 
SI MENG SONG
SI MENG SONG
, 
YAN CHENG
YAN CHENG
, 
ZHI QIANG QIN
ZHI QIANG QIN
, 
GUANG BO GE
GUANG BO GE
, 
BAI LIN AN
BAI LIN AN
 oraz 
PEI GEN XIAO
PEI GEN XIAO
   | 10 gru 2018
Polish Journal of Microbiology's Cover Image
O artykule
Poprzedni artykuł
Następny artykuł
Zacytuj
Article Category: original-paper
Data publikacji: 10 gru 2018
Zakres stron: 417 - 430
Otrzymano: 19 kwi 2018
Przyjęty: 10 lip 2018
DOI: https://doi.org/10.21307/pjm-2018-050
Słowa kluczowe
© 2018 Da Cheng Hao et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

A. Time course of the laccase production in four fungal strains. B. Biomass production, represented by the mycelium dry weight, of fungal strains. The 70 ml culture media contained 20 g/l glucose, 5 g/l peptone, and 5% fungal inoculant. Effects of carbon sources (C)/nitrogen sources (D) on the laccase production of four fungal strains. The agitation rate and the incubation temperature were 120 rpm and 28°C respectively.
A. Time course of the laccase production in four fungal strains. B. Biomass production, represented by the mycelium dry weight, of fungal strains. The 70 ml culture media contained 20 g/l glucose, 5 g/l peptone, and 5% fungal inoculant. Effects of carbon sources (C)/nitrogen sources (D) on the laccase production of four fungal strains. The agitation rate and the incubation temperature were 120 rpm and 28°C respectively.

Fig. 2.

A. RG19 (20 mg/l) decolorization by dye-reducing fungal consortia. B. COD removal by the fungal consortium or the single strain. The mixture of reactive dyes consists of RG19, reactive brilliant blue K-3R and X-BR (each in 6.67 mg/l). Error bars are standard deviations (n = 3).
A. RG19 (20 mg/l) decolorization by dye-reducing fungal consortia. B. COD removal by the fungal consortium or the single strain. The mixture of reactive dyes consists of RG19, reactive brilliant blue K-3R and X-BR (each in 6.67 mg/l). Error bars are standard deviations (n = 3).

Fig. 3.

A. GO annotation of biological process, cellular component and molecular function.
A. GO annotation of biological process, cellular component and molecular function.

Fig. 3.

B. Distribution of Unigene expression levels.
B. Distribution of Unigene expression levels.

Fig. 3.

C. No. of DEGs in three comparisons. In a pairwise comparison, the former one is the control and the latter the treatment.D. Common and unique DEGs of three comparisons.
C. No. of DEGs in three comparisons. In a pairwise comparison, the former one is the control and the latter the treatment.D. Common and unique DEGs of three comparisons.

Fig. 4.

Hierarchical cluster analysis of DEG union set of ordi vs. green, ordi vs. guai, and guai vs. green. The redder the color, the greater the upregulation of the gene expression; the bluer the color, the greater the downregulation of the gene expression.
Hierarchical cluster analysis of DEG union set of ordi vs. green, ordi vs. guai, and guai vs. green. The redder the color, the greater the upregulation of the gene expression; the bluer the color, the greater the downregulation of the gene expression.

Fig. 5.

Proposed degradation pathway of RG19 in M. verrucaria.
Proposed degradation pathway of RG19 in M. verrucaria.

Enzyme activities of M. verrucaria strain DJTU-sh7 in decolorizing RG19.

Enzyme (U/ml)RG19 additionNo RG19
IntracellularExtracellularIntracellularExtracellular
LaccaseND26.241 ± 0.760*ND17.108 ± 0.895
Lignin peroxidase0.164 ± 0.006^0.120 ± 0.006^0.152 ± 0.0170.097 ± 0.002
Mn peroxidaseNDNDNDND
Tyrosinase45.000 ± 3.000^38.667 ± 8.386^42.333 ± 6.42933.667 ± 2.309
Aryl alcohol oxidase14.093 ± 0.056*5.900 ± 0.001*5.244 ± 0.0563.572 ± 0.056
Ferric reductase7.845 ± 0.137**11.788 ± 0.182*6.849 ± 0.0689.000 ± 0.182
Azo reductase22.853 ± 0.544^12.696 ± 0.670^23.242 ± 0.15512.204 ± 0.432
NADH-DCIP reductase3.644 ± 1.547^8.926 ± 0.696^2.603 ± 1.6107.550 ± 2.260
eISSN:
2544-4646
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Microbiology and Virology
Kanał RSS czasopisma