First morphological and molecular identification of third-stage larvae of  (Nematoda: Anisakidae) from marine fishes in Vietnamese water

Hoang Van Hien; Bui Thi Dung; Ha Duy Ngo; Pham Ngoc Doanh

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First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water

oraz

| 01 sty 2021

Journal of Nematology

Tom 53 (2021): Zeszyt 1 (January 2021)

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Data publikacji: 01 sty 2021

Zakres stron: 1 - 9

DOI: https://doi.org/10.21307/jofnem-2021-010

Słowa kluczowe
Anisakid larvae, Intermediate fish host, Molecular analyzes, Morphology, Vietnam

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Study sites along the seashore of Vietnam. Three localities where fishes were infected with Anisakis larvae are print in bold.

Light micrographs of Anisakis typica larva. A. Whole larva; B. Anterior part of the body showing a long ventriculus; C. Anterior part of the body showing a boring tooth; D. Posterior end of the body showing a mucron.

Scanning electron micrographs of Anisakis typica larva. A. Anterior end showing a mouth and a boring tooth; B. Posterior end showing a mucron.

Phylogenetic tree reconstructed from ITS1-5.8S-ITS2 sequences of Anisakis typica from Vietnam and other Anisakis species. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Bootstrap values are shown above the nodes. The nucleotide sequences obtained in this study are printed in bold, and others from the GenBank database are shown with Accession No., species name, and two letter country code of their geographical origin (AU: Australia, BR: Brazil, CN: China, DK: Denmark, GL: Greenland, IN: Indonesia, IE: Ireland, JP: Japan, MU: Mauritius, NO: Norway, PG: Papua New Guinea, PL: Poland, PT: Portugal, TH: Thailand, TR: Turkey, US: United States of America, VN: Vietnam).

Prevalence of Anisakis larvae infection in marine fishes in Vietnamese water.

Locality	No. of fish examined	No. of fish species	Infected fish species	No. of infected/examined fish (%)	Density
Quang Ninh	615	62	0
Hai Phong	478	41	0
Nam Dinh	122	23	0
Nghe An	303	50	0
Quang Binh	520	75	0
Hue	211	28	0
Khanh Hoa	766	82	Dcapterus macarellus	10/20 (50.0)	1-19
			Trichiurus lepturus	6/20 (30.0)	1-5
			Sargocentron rubrum	1/10 (10.0)	1
			Lutjanus johnii	1/10 (10.0)	1
			Megalaspis cordyla	2/12 (16.7)	1; 3
			Priacanthus hamrur	1/8 (12.5)	2
			Pristipomoides filamentosus	3/10 (30.0)	1; 1; 1
Vung Tau	40	6	Megalaspis cordyla	1/5 (20.0)	6
Bac Lieu	390	58	Carangoides malabaricus	1/10 (10.0)	1
Kien Giang	330	63	0
Total	3775	138	8		1-19

Intermediate fish hosts of Anisakis typica in the World and in Vietnam.

No.	Host species	Localities	References
1	Sotalia guianensis
2	Auxis thazard
3	Thunnus thynnus	Brazil Coast
4	Pseudopercis numida
5	Trachurus picturatus
6	Scomber japonicus	Portugal	Mattiucci et al. (2002), Marques et al. (2006), Pantoja et al. (2015)
7	Platichthys flesus
8	Scomberomorus commerson
9	Euthynnus affinis
10	Sarda orientalis	Somalia
11	Coryphaena hippurus
12	Stenella attenuata
13	Globicephala macrorhynchus	Florida	Mattiucci et al. (2005)
14	Scomber scombrus
15	Merluccius merluccius	North Africa	Farjallah et al. (2008)
16	Phycis phycis
	Scomber japonicus	Turkey	Pekmezci et al. (2014)
17	Micromesistius poutassou
	Trichiurus spp.	Japan	Umehara et al. (2010)
	Scomber japonicus		Suzuki et al. (2010)
18	Trichiurus lepturus	Korea	Lee et al. (2009)
19	Todarodes pacificus
20	Astroconger myriaster		Cho et al. (2015)
21	Decapterus macarellus
22	Gerres oblongus
23	Pinjalo lewisi
24	Pinjalo pinjalo	Papua New Guinea	Koinari et al. (2013)
25	Selar crumenophthalmus
26	Scomberomorus maculatus
27	Thunnus albacares
28	Auxis rochei rochei	Indonexia	Palm et al. (2008)
29	Decapterus russelli
30	Nemipterus hexodon	Thailand	Tunya et al. (2020)
31	Nemipterus japonicus
32	Scomber australasicus	Taiwan	Umehara et al. (2010), Sonko et al. (2019)
	Trichiurus lepturus
1	Carangoides malabaricus
2	Dcapterus macarellus
3	Sargocentron rubrum
4	Lutjanus johnii	Vietnam	In this study
5	Megalaspis cordyla
6	Priacanthus hamrur
7	Pristipomoides filamentosus
	Trichiurus lepturus

eISSN:: 2640-396X
Język:: Angielski

Częstotliwość wydawania:: Volume Open
Dziedziny czasopisma:: Life Sciences, other

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First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water

Data publikacji: 01 sty 2021

Zakres stron: 1 - 9

DOI: https://doi.org/10.21307/jofnem-2021-010

Słowa kluczowe
Anisakid larvae, Intermediate fish host, Molecular analyzes, Morphology, Vietnam

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Prevalence of Anisakis larvae infection in marine fishes in Vietnamese water.

Intermediate fish hosts of Anisakis typica in the World and in Vietnam.

First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water

Data publikacji: 01 sty 2021

Zakres stron: 1 - 9

DOI: https://doi.org/10.21307/jofnem-2021-010

Słowa kluczoweAnisakid larvae, Intermediate fish host, Molecular analyzes, Morphology, Vietnam

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Prevalence of Anisakis larvae infection in marine fishes in Vietnamese water.

Intermediate fish hosts of Anisakis typica in the World and in Vietnam.

Słowa kluczowe
Anisakid larvae, Intermediate fish host, Molecular analyzes, Morphology, Vietnam