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Morphological and molecular characterization of Acrobeloides saeedi Siddiqi, De Ley and Khan, 1992 (Rhabditida, Cephalobidae) from India and comments on its status

Aasha Rana
Aasha Rana
, 
Aashaq Hussain Bhat
Aashaq Hussain Bhat
, 
Suman Bhargava
Suman Bhargava
, 
Ashok Kumar Chaubey
Ashok Kumar Chaubey
 oraz 
Joaquín Abolafia
Joaquín Abolafia
   | 28 kwi 2020
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Data publikacji: 28 kwi 2020
Zakres stron: 1 - 21
Otrzymano: 17 paź 2019
DOI: https://doi.org/10.21307/jofnem-2020-027
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© 2020 Aasha Rana et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Acrobeloides saeedi (isolate KMW) (Siddiqi et al., 1992) (line drawing). A: adult neck region; B: anterior end; C: female reproductive system; D: entire male; E: entire female; F: female posterior end; G: male posterior end; H: lateral field.
Acrobeloides saeedi (isolate KMW) (Siddiqi et al., 1992) (line drawing). A: adult neck region; B: anterior end; C: female reproductive system; D: entire male; E: entire female; F: female posterior end; G: male posterior end; H: lateral field.

Figure 2:

Acrobeloides saeedi (Siddiqi et al., 1992) (light microscopy). A: neck (arrow pointing the excretory pore); B: stoma; C: intestinal cardiac part with bacteria; D: entire female; E, F: vagina region in lateral and ventral views, respectively (black arrows pointing the vaginal glands, white arrow pointing the postvulval uterine sac); G: vulva; H: entire male; I: female posterior end; J: male posterior end; K: testis.
Acrobeloides saeedi (Siddiqi et al., 1992) (light microscopy). A: neck (arrow pointing the excretory pore); B: stoma; C: intestinal cardiac part with bacteria; D: entire female; E, F: vagina region in lateral and ventral views, respectively (black arrows pointing the vaginal glands, white arrow pointing the postvulval uterine sac); G: vulva; H: entire male; I: female posterior end; J: male posterior end; K: testis.

Figure 3:

Acrobeloides saeedi (Siddiqi et al., 1992) (scanning electron microscopy). A-B: male lip region (arrows pointing the amphids); C-F: female lip region.
Acrobeloides saeedi (Siddiqi et al., 1992) (scanning electron microscopy). A-B: male lip region (arrows pointing the amphids); C-F: female lip region.

Figure 4:

Acrobeloides saeedi (Siddiqi et al., 1992) (scanning electron microscopy). A: cuticle at excretory pore level (arrow); B, C, F, G: male posterior end in left lateral (B, F) and ventral (C, G) views (black arrows pointing the genital papillae, white arrows pointing the phasmids); D: lateral fields (arrows pointing the longitudinal incisures); E: female posterior end (arrow pointing the phasmid).
Acrobeloides saeedi (Siddiqi et al., 1992) (scanning electron microscopy). A: cuticle at excretory pore level (arrow); B, C, F, G: male posterior end in left lateral (B, F) and ventral (C, G) views (black arrows pointing the genital papillae, white arrows pointing the phasmids); D: lateral fields (arrows pointing the longitudinal incisures); E: female posterior end (arrow pointing the phasmid).

Figure 5:

Bayesian Inference tree from known and the newly sequenced Acrobeloides saeedi based on sequences of the 18 S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.
Bayesian Inference tree from known and the newly sequenced Acrobeloides saeedi based on sequences of the 18 S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.

Figure 6:

Bayesian Inference tree from known and the newly sequenced Acrobeloides saeedi based on sequences of the 28 S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.
Bayesian Inference tree from known and the newly sequenced Acrobeloides saeedi based on sequences of the 28 S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.

Morphometric data for Acrobeloides saeedi DH1 isolated from Galleria culture.

Characters Female Male Juveniles
n 20 20 27
Total body length 1271 ± 112 (1060–1446) 959 ± 74 (798–1144) 474 ± 54 (404–636)
a 14.1 ± 1.3 (11.5–16.6) 20 ± 1.8 (17.1–24.0) 21  ±  3.5 (15.3–28.0)
b 6.9 ± 0.4 (6.1–7.8) 5.0 ± 0.3 (4.2–5.6) 3.8 ± 0.36 (3.2–4.9)
c 27 ± 2.2 (22.0–30.0) 27 ± 3.0 (22–34) 13 ± 2.3 (5.7–17.6)
c' 1.7 ± 0.2 (1.5–2.4) 1.6 ± 0.1 (1.3–1.8) 2.7 ± 0.7 (2.0–6.1)
V 71 ± 3.6 (60–77) – –
Lip length 4.8 ± 0.7 (3–6) 3.8 ± 0.5 (3–5) 2.8 ± 0.5 (2–4)
Lip region width 7.8 ± 1.4 (5–10) 6.3 ± 0.7 (5–8) 5.1 ± 0.7 (4–7)
Stoma length 13.1 ± 1.2 (11–16) 12.2 ± 1.6 (8–15) 10.4 ± 1.9 (8–15)
Pharyngeal corpus 107 ± 9.8 (86–125) 113 ± 6.7 (99–123) 85 ± 8.8 (64–100)
Isthmus 26 ± 4.5 (16–34) 28 ± 4.4 (21–36) 18.2 ± 4.0 (9–27)
Basal bulb length 37 ± 3.6 (30–43) 34 ± 4.1 (26–40) 21 ± 2.9 (16–30)
Pharynx length 170 ± 10.3 (155–188) 174 ± 8.4 (146–184) 122 ± 11.9 (105–142)
Nerve ring – ant. end 112 ± 6.6 (98–124) 131 ± 8.0 (114–143) 82 ± 9.1 (65–102)
Excretory pore – ant. end 134 ± 12.6 (115–161) 157 ± 12.5 (141–192) 95 ± 11.8 (77–123)
Deirid – ant. end 127 ± 17.3 (95–159) 125 ± 7.5 (105–139) ?
Neck length 184 ± 10.2 (170–202) 191 ± 8.1 (175–207) 125 ± 9.4 (108–153)
Body diam. at midbody 90 ± 10.9 (70–108) 47 ± 3.1 (40–54) 23 ± 3.4 (18–31)
Ovary length 437 ± 51 (348–532) – –
Spermatheca length 50 ± 10.4 (39–68) – –
Uterus length 385 ± 85 (256–537) – –
Postvulval uterine sac length 92 ± 8.6 (73–101) – –
Vagina length 23 ± 1.83 (19–25) – –
Body diam. at vulva level 75 ± 10.8 (56–91) – –
Vulva – anterior end 904 ± 87 (749–1043) – –
Rectum length 33 ± 4.8 (22–43) – 13.4 ± 2.2 (10–18)
Body diam. at anus 28 ± 2.4 (21-32) 23 ± 2.7 (20-28) 14.3 ± 1.6 (11-17)
Tail length 48 ± 3.8 (41-54) 36 ± 2.5 (32-40) 38 ± 9.9 (29-80)
Phasmid to anus distance 27 ± 4.0 (21-37) 21 ± 2.1 (18–25) –
Spicule length – 45 ± 2.8 (41-50) –
Gubernaculum length – 26 ± 2.0 (22-30) –

Comparative morphometrics of males from populations of Acrobeloides maximus – group (all measurements in µm except L in mm).

Species A. saeedi (KMW-DH1) A. longiuterus A. longiuterus (as camberenensis) A. longiuterus (as A. camberenensis) A. longiuterus A. saeedi (as A. gossypii) A. saeedi (as A. gossypii) A. bodenheimeri A. bodenheimeri A. bodenheimeri A. bodenheimeri A. bodenheimeri A. bodenheimeri A. bodenheimeri (as A. mushtaqi) A. maximus
Reference Present paper Rashid and Heyns (1990) De Ley et al. (1990) De Ley et al. (1999) Abolafia and Peña-Santiago, 2017a, b Nahiyoon et al. (2019) Nahiyoon et al. (2019) Steiner (1936); Siddiqi et al. (1992) Andrássy (1967) Siddiqi et al. (1992) De Ley et al. (1999) Abolafia, Peña-Santiago (2002) Mehdizadeh et al. (2013) Pervez (2011) Steiner (1935)
Country (State) India Namibia Senegal USA Namibia Pakistan Pakistan Israel Mongolia Malawi USA Spain Iran India USA
n (♂) 40 26 6 20 6 15 15 8 6 10 10 2 4 10 1
L 0.79–1.16 0.53–0.94 0.54–0.65 0.70–1.03 0.68–0.84 0.71–1.39 0.71–1.39 0.63–0.71 0.56–0.59 0.56–0.87 0.98–1.18 0.69, 0.73 0.70–0.86 0.61–0.62 0.9
a 13.0–24.0 16.7–26.4 20.2–21.7 16.0–26.0 17.8–23.7 11.1–16.6 11.1–16.6 14.0–16.0 12.0–15.0 22–26 20.0–25.0 24.0, 25.0 22.0–30.0 27.0–31.0 27.0
b 4.2–7.0 4.0–5.9 4.0–4.8 4.3–6.8 4.1–5.1 4.6–7.5 4.6–7.5 4.9–5.1 4.2–4.8 4.3–5.4 6.0–7.8 5.0, 5.2 4.8–5.4 4.0–4.2 7.2
c 21.0–34.0 14.4–21.7 14.9–18.0 17.0–23.0 17.3–20.3 19.5–27.8 19.5–27.8 15–18 14.0–16.0 15–18 20.0–24.0 18.8, 16.6 14.0–18.0 16.0–18.0 18.0
c’ 1.2–2.2 1.4–2.1 1.6–2.0 1.2–1.6 1.2–1.5 0.9–1.3 0.9–1.3 ? 1.9* 2.1* 1.3–1.7 1.3, 1.7 1.6–2.0 1.4–1.8 1.8*
Lip region width 5–8 ? ? ? ? 11–14 11–14 ? ? ? ? 3, 4 3–4 ? ?
Stoma length 8–17 ? 08–10 11–13 11–12 12–15 12–15 ? ? ? 10–14 11, 12 10–11 11–12 ?
Procorpus length 28–59 ? ? 58–66 ? ? ? ? ? ? ? 11, 11 12–14 ? ?
Metacorpus length 45–75 ? ? 32–40 ? ? ? ? ? ? ? ? ? ? ?
Isthmus length 21–50 ? 15–25 16–23 19–22 16–20 16–20 22–23 ? ? ? ? 17–32 ? ?
Bulb length 26–41 ? 16–23 17–27 22–28 24–30 24–30 ? ? ? 22–30 22, 23 20–26 ? ?
Pharynx length 142–184 ? 122–151* 205–260 122–161 150–188 150–188 129–143 ? ? 210–293 100, 119 131–149 145–152 ?
Nerve ring – ant. End 88–143 ? 90–104 108–135 120–132 112–134 112–134 100–115 ? ? 117–151 140, 140 110–127 106–110 ?
Excretory pore – ant end 112–192 ? 78–91 121–175 119–141 132–144 132–144 ? ? ? 135–192 121, ? 128–143 ? ?
Neck length 156–207 130–174 128–148 ? 156–168 162–203* 162–203* ? ? ? 133–188 143–160 ? ?
Midbody diam. 40–73 ? 25–31 39–51 35–42 64–88 64–88 ? ? ? 44–57 33, 40 24–40 19–22 ?
Anal body diam. 18–34 ? 20–23 27–35 27–34 32–40 32–40 ? ? 25* 31–35 18, 22 24–27 17–24 ?
Tail length 32–48 37–50 36–42 39–47 38–44 40–50 40–50 38–43 ? 54–80 40–58 22, 25 43–54 34–37 ?
Spicules length 41–54 29–51 29–34 40–48 41–46 38–57 38–57 39–44 30–40 35–43 42–50 37 38–41 39–43 ?
Gubernaculum length 21–30 18–35 14–19 24–30 26–31 24–35 24–35 22–28 20 19–24 27–34 23, 22 19–28 23–24 ?
D% (EP/ES×100) 69–94 ? ? 57–67* 92* 82* 82* ? ? ? ? 97* ? ?
E% (EP/T×100) 242–577 ? ? 310–372* 317* 306* 306* ? ? ? ? 233, 300* 279* ? ?
SW% (SL/ABD×100) 150–294 ? ? 151* 143* 126* 126* ? ? ? ? 137* 155* 194* ?
GS% (GL/SL×100) 40–70 ? ? 67* 66* 55* 55* ? ? ? ? 65* 59* 58* ?

Pairwise distances of the D2D3 regions of 28 S rDNA regions between present strains of Acrobeloides and already described species.

S. No. 28S rDNA Country 1 2 3 4 5 6 7 8 9 10 11 12 13 14
1 MK935147 A. saeedi KMW India 1 1 5 5 5 38 51 59 67 74 75 76 76
2 MN101167 A. saeedi DH1 India 100 0 5 5 5 38 51 59 68 74 75 77 77
3 KY914573 A. saeedi Iran 100 100 10 10 10 38 56 64 68 79 80 82 82
4 MF325168 A. sexlineatus Germany 98.9 98.9 98.0 0 0 5 8 0 11 0 0 0 0
5 MF325157 A. buchneri Germany 98.9 98.9 98.0 100 0 5 8 0 11 0 0 0 0
6 DQ903087 A. tricornis Germany 95.1 95.0 94.7 100 100 5 8 0 11 0 0 0 0
7 AF147069 A. longiuterus USA 96.7 96.7 96.8 99.0 99.0 96.9 30 35 45 34 35 36 36
8 AF147067 A. maximus USA 95.6 95.5 95.2 98.5 98.5 96.1 97.5 43 51 44 45 46 46
9 KX889089 A. varius South Korea 94.6 94.6 94.3 100 100 99.8 97.0 96.3 41 1 0 3 3
10 DQ145625 A. bodenheimeri Belgium 95.8 95.6 95.7 97.7 97.7 96.9 96.1 95.6 96.4 50 49 50 50
11 DQ903076 A. nanus Sweden 95.2 95.2 94.9 100 100 99.9 97.1 96.3 99.9 96.9 1 2 2
12 DQ903083 A. thornei USA 95.1 95.1 94.9 100 100 99.8 97.0 96.2 100 96.9 99.9 3 3
13 DQ145624 A. ellesmerensis USA 95.1 95.0 94.8 100 100 100 96.9 96.1 99.8 96.9 99.9 99.8 0
14 DQ903081 A. buetschlii USA 95.1 95.0 94.8 100 100 100 96.9 96.1 99.8 96.9 99.9 99.8 100

Morphometric data for Acrobeloides saeedi KMW isolated from Galleria culture.

Characters Female Male Juvenile
n 20 20 27
Total body length 1387 ± 63 (1307–1566) 987 ± 89 (812–1156) 653 ± 20 (626–704)
a 14.8 ± 1.4 (12.8–17.4) 21 ± 2.7 (13.0–24.0) 22 ± 1.7 (19.6–28.0)
b 7.8 ± 0.7 (6.9–10.0) 5.9 ± 0.6 (4.7–7.0) 4.4 ± 0.2 (4.0–5.0)
c 26 ± 2.2 (22.0–33.0) 25 ± 2.7 (21.0–30.0) 17 ± 1.2 (15.0–21.0)
c’ 1.8 ± 0.2 (1.5–2.3) 1.7 ± 0.2 (1.2–2.2) 2.0 ± 0.2 (1.4–2.6)
V 70 ± 2.1 (66–74) – –
Lip length 5.0 ± 0.6 (4–7) 4.6 ± 0.8 (3–6) 4.3 ± 0.7 (3–6)
Lip region width 8.9 ± 0.8 (8–11) 6.3 ± 0.7 (5–8) 5.0 ± 0.5 (4–5)
Stoma length 12.9 ± 2.2 (9–15) 14.6 ± 1.9 (11–17) 12 ± 1.6 (7–14)
Pharyngeal corpus length 108 ± 8.1 (88–124) 93 ± 8.9 (81–108) 82  ±  7.4 (69–99)
Isthmus length 37 ± 4.4 (28–46) 35 ± 6.0 (28–50) 29 ± 4.4 (21–37)
Basal bulb length 39 ± 4.9 (31–53) 34 ± 3.4 (28–41) 24 ± 1.5 (21–27)
Pharynx length 184 ± 11.1 (159–202) 161 ± 12.9 (142–181) 136 ± 7.8 (116–149)
Nerve ring – ant. end 113 ± 14 (91–151) 106 ± 10.2 (88–129) 94 ± 8.4 (76–109)
Excretory pore – ant. end 138 ± 11.0 (112–157) 129 ± 12.4 (112–165) 111 ± 8.9 (94–127)
Deirid – ant. end 155 ± 13.4 (130–178) 128 ± 11.9 (111–156) –
Neck length 191 ± 9.7 (168–208) 181 ± 12.8 (156–203) 162 ± 12.4 (130–181)
Body diam. at midbody 95 ± 10.0 (80–112) 49 ± 7.7 (40–73) 30 ± 2.1 (24–33)
Ovary length 542 ± 60 (401–652) – –
Spermatheca length 49 ± 9.8 (33–61) – –
Uterus length 280 ± 46 (211–376) – –
Postvulval uterine sac length 100 ± 11.3 (85–112) – –
Vagina length 26 ± 6.2 (17–35) – –
Body diam. at vulval level 84 ± 5.7 (74–93) – –
Vulva – anterior end 976 ± 36 (896–1046) – –
Rectum length 24 ± 5.5 (12–32) – 19.5 ± 2.1 (14–22)
Body diam. at anus 31 ± 3.4 (22–39) 24 ± 3.3 (18–34) 20 ± 2.7 (16–31)
Tail length 54 ± 4.1 (44–62) 39 ± 3.5 (34–48) 40 ± 2.9 (31–45)
Phasmid to anus distance 30 ± 3.4 (26–35) 28 ± 3.9 (21–36) –
Spicules length – 48 ± 4.2 (41–54) –
Gubernaculum length – 25 ± 2.8 (21–30) –

Comparative morphometrics of females from populations of Acrobeloides maximus – group (all measurements in µm except L in mm).

Species A. saeedi (KMW-DH1) A. saeedi A. saeedi A. saeedi (as A. ishraqi) A. saeedi (as A. gossypii) A. saeedi (as A. gossypii) A. bodenheimeri A. bodenheimeri A. bodenheimeri (as A. rotundifolius) A. bodenheimeri A. bodenheimeri A. bodenheimeri A. bodenheimeri A. bodenheimeri (as A. mushtaqi) A. longiuterus A. longiuterus (as A. camberenensis) A. longiuterus (as A. camberenensis) A. longiuterus A. maximus Paracephalobus litoralis
Reference Present paper Siddiqi et al. (1992) Khan and Hussain (1997) Pervez (2011) Nahiyoon et al. (2019) Nahiyoon et al. (2019) Steiner (1936); Siddiqi et al. (1992) Andrássy (1967) Bussau (1991) Siddiqi et al. (1992) De Ley et al. (1999) Abolafia and Peña-Santiago (2002) Mehdizadeh et al. (2013) Pervez (2011) Rashid and Heyns (1990) De Ley et al. (1990) De Ley et al. (1999) Abolafia and Peña-Santiago, 2017a, b Thorne (1925) Akhtar (1962)
Country India Pakistan Pakistan India Pakistan Pakistan Israel Mongolia Denmark Malawi USA Spain Iran India Namibia Senegal USA Namibia USA Pakistan
n (♀) 40 30 20 10 15 15 8 12 1 22 30 4 3 10 21 5 15 3 1 1
L 1.06–1.57 0.88–1.21 0.86–1.20 0.99–1.19 0.80–1.70 0.8–1.70 0.63–0.78 0.67–0.77 0.88 0.64–0.91 0.86–1.53 0.70–0.91 0.63–0.72 0.61–0.68 0.57–0.88 0.45–0.67 0.88–1.19 0.74–0.84 1.2 0.8
A 11.5–17.4 21.0–30.0 21.0–30.0 10.5–15.8 8.1–15.5 8.1–15.5 15.0–16.0 15.0–17.0 24.4 17–23 16.0–23.0 17.5–22.9 21.0–22.0 31.8–33.1 16.8–24.0 18.3–19.6 15.0–19.0 18.1–22.8 20.0 23.2
B 6.1–10.0 4.6–5.9 4.86–5.8 4.8–6.1 4.5–8.0 4.5–8.0 5.0–5.6 4.8–5.5 4.7 4.7–5.6 5.6–7.8 4.5–5.5 4.2–5.1 4.2–4.4 3.5–5.3 3.4–4.6 5.7–6.9 4.4–5.0 5.7 6.0
C 22.0–33.0 20.0–26.0 20–26 20.6–23 16.0–29.2 16–29.2 17–21 16.0–19.0 18.1 16.0–18.3 20.0–30.0 19.0–20.3 16.0–20.0 14.3–17.9 16.2–21.8 15.1–18.0 20.0–26.0 16.5–19.6 18.0 17.7
c' 1.5–2.4 1.8–2.4 1.8–2.3 1.4–1.8 1.1–1.9 1.1–1.9 ? 1.7–2.1 2.1* 2.0–2.5 1.4–2.1 1.6–2.1 1.8–2.1 1.2–1.8 1.4–2.3 2.0–2.4 1.4–1.9 1.7–2.1 2.0 2.5–2.7*
V 60–77 65–74 ? 68–74 70–75 70.2–75 69–71 65–71 69 67–71 67–71 64–69 67–70 77* 64–72 65–70 84* 66–70 70 65
Lip region width 5–11 11–13 11–13 11–13 9–10 12–15 9–10 ? 6.7* 5.9* 9* 3 10–11 8.6–12 ? ? ? 5.5* ? 7*
Stoma length 9–18 12* 12–18 16–16 12–14 12–22 12–14 ? 20* 14.7* 11–16 11–14 13–14 8–12 11–14 8–10 12–15 10–12 20 10*
Procorpus 37–69 ? ? ? ? ? ? ? ? ? 50–79 ? ? ? ? ? 60–85 ? ? ?
Metacorpus 43–80 ? ? ? ? ? ? ? ? ? 30–49 ? ? ? ? ? 35–46 ? ? ?
Isthmus 16–46 21.9* 20* ? 20–26 14–28 20–26 ? 23* 22–36 20–37 22–25 21–25 27.6* 14–15* 15–24 17–23 15 –23 ? ?
Bulb length 30–53 29–34 29–34 24–38 19–21 26–32.5 19–21 ? 19.8* 19–21 22–32 22–27 19–26 24* ? 15–21 22–28 24–25 ? 13–25
Pharynx length 155–202 190–218 190–218 179–223 129–157 166–228 129–157 ? 157* 135–178 205–316* 135–166 133–141 145–154 ? 123 –159 229–313* 137–164 210 8–14
Nerve ring–ant. end 91–151 163* ? 151* 92–114 125–136 92–114 ? 132* 106* 113–174 107–126 109–117 101–105 89–142 82–108 125–148 119–135 162 78*
Excretory pore–ant. end 112–161 140–188 140–188 155–174 ? 136–150 ? ? 139* 118–134 131–209 114–148 123–135 ? 89–157 83–118 154–195 127–142 ? 81*
Neck region 168–208 215* 202–236* 194–239* ? 178–250* ? ? 177* 137* 157–332* 146–180 139–150 153–166* 120–177 134–153 241–327* 149–172 ? ?
Midbody diam. 70–112 56.6* ? 59–113 ? 52–170 ? ? 40* 39* 49–73 33–40 30–34 18–21 ? 13–17 49–72 35–41 ? 29.4*
Vulva – anterior end 749–1046 680–840 573* 637* 503–550 616–1225 503–550 ? 650* 503–550 1001* 447–629 436–491 495.2* ? ? 875* ? 840 291.6*
Body diam. at anus 21–39 29* 19* 28–36 ? 25–50 ? ? 30* 18* 22–31 18–22 19–20 19–21 ? 20–23 25* 21–25 34 15*
Rectum length 12–43 37.5* ? 31–42 ? 24–30 ? ? ? 19* 27–42 22–25 21–29 17.2* ? ? 23–32 17.8 ? ?
Tail length 41–62 39–50 39–50 10–15 35–45 42–55 35–45 ? 50* 39–56 41–60 36–45 35–40 37–42 61–111 30–37 41–52 88–91 66 39–43
Phasmid to anus distance 21–39 10–19 23.3* ? ? ? ? ? ? 18.8* 22–36 16–29 17.5* 20.7* 41–66 ? 21–32 56–64 ? ?
D% (EP/ES×100) 64–101 74–86* 74–86* 82* 79* 79* ? ? 88* 80.5* 64–66 87* 94* ? ? ? 68* 88* ? ?
E% (EP/T×100) 206–320 359–376* 359–376* ? ? 285* ? ? 278* 265.3* 312–348 323* 344* ? ? ? 378* 150* ? ?

Pairwise distances of the 18 S rDNA regions between present strains of Acrobeloides and already described species.

S. No. 18S rDNA Country 1 2 3 4 5 6 7 8 9 10 11
1 MK935150 A. saeedi KMW India 0 0 9 19 19 19 19 24 30 89
2 MK935149 A. saeedi DH1 India 100 0 9 19 19 19 19 24 30 89
3 KY090631 A. saeedi Iran 100 100 9 19 19 19 19 31 23 89
4 MK541681 A. tricornis Germany 98.5 98.5 98.3 0 0 0 0 10 10 9
5 DQ102707 A. nanus UK 98.5 98.5 98.5 100 16 2 0 39 13 97
6 KX889085 A. varius South Korea 98.5 98.5 98.5 100 99.3 2 0 39 13 96
7 AY284673 A. apiculatus Netherlands 98.5 98.5 98.5 100 99.9 99.9 0 37 13 95
8 MF325099 A. buchneri Germany 98.4 98.4 98.5 100 100 100 100 19 4 86
9 AF202159 A. bodenheimeri France 98.1 98.1 97.4 98.6 98.3 98.3 98.4 98.4 32 92
10 KY119635 A. thornei Ireland 97.5 97.5 98.1 98.2 99.0 99.0 99.0 99.7 97.4 95
11 JQ237850 A. maximus USA 92.3 92.3 91.7 98.8 95.0 95.1 95.1 92.0 95.3 91.3
eISSN:
2640-396X
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Life Sciences, other
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