<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Advances in sequencing technologies have accelerated our understanding of the complex genetic network of organisms and genomic divergences that are linked to evolutionary processes. While many model organisms and laboratory strains have been sequenced, wild populations are underrepresented in the growing list of sequenced genomes. Here, we present a <italic>de novo</italic> assembly of <italic>Steinernema feltiae</italic>, strain NW, collected from a working agricultural field in south central Washington, USA. Leveraging Pacific Biosciences (PacBio) long reads, we sequenced strain NW to a high depth (99×). The resulting <italic>de novo</italic> assembly is significantly larger than the previous assembly generated from the laboratory strain SN, with a noticeable improvement in continuity and completeness. Comparative analysis of two assemblies revealed numerous single nucleotide polymorphisms (SNPs), breakpoints, and indels present between the two genomes. This alternative genome resource and annotation could benefit the research community to examine the genetic foundation of evolutionary processes as well as genomic variation among conspecific populations.</p>
</abstract>

Advances in sequencing technologies have accelerated our understanding of the complex genetic network of organisms and genomic divergences that are linked to evolutionary processes. While many model organisms and laboratory strains have been sequenced, wild populations are underrepresented in the growing list of sequenced genomes. Here, we present a de novo assembly of Steinernema feltiae, strain NW, collected from a working agricultural field in south central Washington, USA. Leveraging Pacific Biosciences (PacBio) long reads, we sequenced strain NW to a high depth (99×). The resulting de novo assembly is significantly larger than the previous assembly generated from the laboratory strain SN, with a noticeable improvement in continuity and completeness. Comparative analysis of two assemblies revealed numerous single nucleotide polymorphisms (SNPs), breakpoints, and indels present between the two genomes. This alternative genome resource and annotation could benefit the research community to examine the genetic foundation of evolutionary processes as well as genomic variation among conspecific populations.

A draft genome of a field-collected <italic>Steinernema feltiae</italic> strain NW

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A draft genome of a field-collected  strain NW

running-head

Advances in sequencing technologies have accelerated our understanding of the complex genetic network of organisms and genomic divergences that are linked to...

A draft genome of a field-collected Steinernema feltiae strain NW

Data publikacji: 17 mar 2020

Zakres stron: 1 - 7

Otrzymano: 23 lip 2019

DOI: https://doi.org/10.21307/jofnem-2020-003

Słowa kluczowe
Entomopathogenic nematode, Biological control, Genome,

© 2020 Zhen Fu et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A draft genome of a field-collected Steinernema feltiae strain NW

Data publikacji: 17 mar 2020

Zakres stron: 1 - 7

Otrzymano: 23 lip 2019

DOI: https://doi.org/10.21307/jofnem-2020-003

Słowa kluczoweEntomopathogenic nematode, Biological control, Genome,

© 2020 Zhen Fu et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
Entomopathogenic nematode, Biological control, Genome,