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Figure 1:
(A) Chemotaxis assays performed on S. carpocapsae IJs after exposure to live and freeze-killed host cuticles, where +1 indicates strong attraction, near 0 indicates indifference, and −1 indicates strong repulsion. (B) Participation evaluation of S. carpocapsae IJs after exposure to live and freeze-killed host cuticles, where ‘to host volatiles’ indicates attraction, ‘middle’ indicates indifference, and ‘to control’ indicates repulsion toward our test volatile. Statistical significance was determined with an unpaired, one-way ANOVA test for CI, and two-way ANOVA testing for participation. Error bars represent SEM. ** P < 0.01; *** P < 0.001; **** P < 0.0001.
Figure 2:
The chemotaxis index of S. carpocapsae IJs responding to four host-associated chemicals, where +1 indicates strong attraction, 0 indicates indifference, and −1 indicates strong repulsion. Statistical significance was determined with an unpaired, ordinary, one-way ANOVA test. Error bars represent SEM.
Figure 3:
Graphs of IJ participation in response to p-Cresol (4-methylphenol) (A), Trimethylamine (B), Prenol (C), and Tetrahydrofuran (D). We tested IJs exposed to live host cuticles and non-exposed IJs. The IJs either traveled toward the test volatile, stayed in the middle region, or moved away from the test volatile and toward the control.