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Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination


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Fig. 1

Line drawings of Aphelenchoides giblindavisi n. sp. (A, B) Entire body of female and male. (C) Anterior end. (D, E) Pharynx. (F) Part of female reproductive system. (G) Tail end. (H, I) Tail of female. (J) Posterior body region of male.
Line drawings of Aphelenchoides giblindavisi n. sp. (A, B) Entire body of female and male. (C) Anterior end. (D, E) Pharynx. (F) Part of female reproductive system. (G) Tail end. (H, I) Tail of female. (J) Posterior body region of male.

Fig. 2

Light microphotographs of Aphelenchoides giblindavisi n. sp. (A–C) Anterior region. (D) Part of female reproductive system (junction of oviduct and spermatheca). (E) Spicule. (F) Vulva region and postvulval uterine sac. (G, H) Posterior body region of female. (I) Tail end of male (All scale bars = 10 μm).
Light microphotographs of Aphelenchoides giblindavisi n. sp. (A–C) Anterior region. (D) Part of female reproductive system (junction of oviduct and spermatheca). (E) Spicule. (F) Vulva region and postvulval uterine sac. (G, H) Posterior body region of female. (I) Tail end of male (All scale bars = 10 μm).

Fig. 3

Scanning electron microphotographs of Aphelenchoides giblindavisi n. sp. (A) Details of anterior region. (B, C) Lip region. (D, E) Lateral lines in different parts of mid-body. (F) Details of tail end. (G) Female posterior body region. (H, I) Male caudal region.
Scanning electron microphotographs of Aphelenchoides giblindavisi n. sp. (A) Details of anterior region. (B, C) Lip region. (D, E) Lateral lines in different parts of mid-body. (F) Details of tail end. (G) Female posterior body region. (H, I) Male caudal region.

Fig. 4

Scanning electron microphotograph of Aphelenchoides giblindavisi n. sp. Details of lip region. Abbreviations: il9–inner lip papillae, aa–amphidial openings, olp–outer labial papillae, cp–cephalic papillae, ld–labial disc.
Scanning electron microphotograph of Aphelenchoides giblindavisi n. sp. Details of lip region. Abbreviations: il9–inner lip papillae, aa–amphidial openings, olp–outer labial papillae, cp–cephalic papillae, ld–labial disc.

Fig. 5

Line drawings of Iranian population of Robustodorus helicus n. comb. (A) Anterior end. (B, C) Female total body. (D, F) Pharynx. (E) Stylet. (G, H) Posterior body region.
Line drawings of Iranian population of Robustodorus helicus n. comb. (A) Anterior end. (B, C) Female total body. (D, F) Pharynx. (E) Stylet. (G, H) Posterior body region.

Fig. 6

Light microphotographs of fresh females of Iranian population of Robustodorus helicus n. comb. (A) Anterior region. (B, H) Part of pharynx. (C) Anterior end. (D) Excretory pore. (E) Metacorpus. (F) Tail. (G) Embryo inside the mature egg inside the body of female. (All scale bars = 10 μm).
Light microphotographs of fresh females of Iranian population of Robustodorus helicus n. comb. (A) Anterior region. (B, H) Part of pharynx. (C) Anterior end. (D) Excretory pore. (E) Metacorpus. (F) Tail. (G) Embryo inside the mature egg inside the body of female. (All scale bars = 10 μm).

Fig. 7

Light microphotographs of Iranian population of Robustodorus helicus n. comb. (A) Three tulip-shaped lips in lateral view. (B, C) Three lines in the lateral field forming two bands. (D, E) Postvulval uterine sac. (F) Anterior region. (All scale bars = 10 μm).
Light microphotographs of Iranian population of Robustodorus helicus n. comb. (A) Three tulip-shaped lips in lateral view. (B, C) Three lines in the lateral field forming two bands. (D, E) Postvulval uterine sac. (F) Anterior region. (All scale bars = 10 μm).

Fig. 8

Bayesian tree inferred under the GTR + G + I model using SSU rDNA sequences of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font.
Bayesian tree inferred under the GTR + G + I model using SSU rDNA sequences of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font.

Fig. 9

Bayesian tree inferred under the GTR + G + I model using partial LSU rDNA sequence of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font. The asterisk on Laimaphelenchus hyrcanus Miraeiz et al. (2015) refers to the possible synonymy of the species with L. belgradiensis Oro, 2015 as discussed by Pedram et al. (2018a, 2018b).
Bayesian tree inferred under the GTR + G + I model using partial LSU rDNA sequence of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font. The asterisk on Laimaphelenchus hyrcanus Miraeiz et al. (2015) refers to the possible synonymy of the species with L. belgradiensis Oro, 2015 as discussed by Pedram et al. (2018a, 2018b).

Morphometrics of Aphelenchoides giblindavisi n. sp. from Iran. All measurements are in μm and in the form: mean ± S.D. (range).

Female Male
Character Holotype Paratypes Paratypes
n 12 12
L 653 671 ± 72.4 (546–795) 632 ± 41.4 (523–679)
a 39.6 34.9 ± 2.2 (31.4–39.6) 37.4 ± 2.2 (32.4–41.0)
b 8.6 8.9 ± 0.8 (7.4–10.1) 8.7 ± 0.4 (8.0–9.4)
b′ 4.5 4.5 ± 0.4 (3.9–5.0) 4.5 ± 0.2 (4.2–5.0)
c 18.7 18.9 ± 1.0 (17.2–20.4) 18.9 ± 1.7 (15.8–21.5)
c′ 3.5 3.4 ± 0.2 (3.1–3.7) 2.8 ± 0.2 (2.6–3.1)
V or T 70.1 70.6 ± 1.6 (68.8–73.5) 52.8 ± 3.4 (47.4–59.2)
Head height 2.5 2.5 ± 0.0 (2.0–2.5) 2.5 ± 0.0 (2.0–2.5)
Head diam. 5.5 5.5 ± 0.4 (5–6) 5.5 ± 0.3 (5–6)
Stylet 10.5 10.5 ± 0.5 (10–11) 10.0 ± 0.4 (10–11)
Stylet conus 4.5 4.5 ± 0.2 (4.0–4.5) 4.0 ± 0.2 (3.5–4.5)
m 42.9 38.9 ± 2.3 (36.4–42.9) 38.4 ± 2.1 (35–40)
Median bulb 58 58.0 ± 3.2 (54–65) 56.0 ± 1.4 (54–58)
Excretory pore 69 69.0 ± 6.2 (60–84) 66.5 ± 1.8 (64–69)
Hemizonid 80 89.0 ± 10.6 (77–110) 81.0 ± 2.6 (78–85)
Pharynx 76 75.5 ± 2.8 (71–81) 72.0 ± 3.4 (65–78)
Nerve ring 75 75.0 ± 2.8 (71–81) 73.0 ± 2.3 (70–78)
Median bulb length 13.5 14 ± 1 (12–15) 13.0 ± 0.9 (11–14)
Median bulb diam. 10.5 11.0 ± 1.1 (9.0–12.5) 10.0 ± 0.9 (8.0–11.5)
Median bulb length/diam. 1.3 1.3 ± 0.1 (1.1–1.4) 1.3 ± 0.1 (1.2–1.6)
Pharyngeal overlapping 70 73.0 ± 6.7 (63–87) 67.0 ± 6.4 (60–80)
Maximum body diam. 16.5 19.0 ± 2.4 (15–23) 17.0 ± 1.4 (14–19)
Vulval body diam. (VBD) 16 18.0 ± 1.8 (14.5–20.0)
Body diam. at median bulb 13.5 14.0 ± 1.4 (12.0–15.5) 13.0 ± 0.8 (11–14)
Postvulval uterine sac (PUS) 65 62.4 ± 6.0 (52–69)
PUS/VBD 4.1 3.5 ± 0.4 (2.7–4.1)
Vulva–anus 160 161 ± 21.6 (114–190)
Ovary or testis length 295 257 ± 58.7 (183–356) 334 ± 35 (248–394)
Anal (cloacal) body width 10 10 ± 1 (9.0–12.5) 12.0 ± 0.6 (10.5–13.0)
Tail length 35 35.0 ± 2.5 (30–39) 34.0 ± 1.8 (31–37)
Spicules length (arc line) 17.0 ± 0.7 (16–18)
Capitulum 7.0 ± 0.5 (6–8)

Morphometrics of Iranian population of Robustodorus helicus n. comb. All measurements are in μm and in the form: mean ± S.D. (range).

Females
n 15
L 464 ± 37.3 (395–547)
a 29.2 ± 1.8 (26.7–33.5)
b 7.8 ± 0.6 (6.7–8.8)
b′ 4.6 ± 0.4 (3.8–5.4)
c 19.7 ± 1.7 (16.8–22.8)
c′ 2.8 ± 0.3 (2.4–3.1)
V 69.3 ± 1.6 (64.4–71.1)
Head height 2.0 ± 0.3 (2.0–2.5)
Head diam. 5.0 ± 0.4 (4.5–5.5)
Stylet 10.0 ± 0.4 (10–11)
Stylet conus 4.0 ± 0.3 (4.0–4.5)
m 41.3 ± 2.8 (36.4–45.0)
Median bulb 47.5 ± 2.9 (39–51)
Excretory pore 53.0 ± 4.8 (46–62)
Hemizonid 63.0 ± 5.6 (53–69)
Pharynx 60 ± 4 (49–65)
Nerve ring 58 ± 4 (45–62)
Median bulb length 11.5 ± 0.9 (10.0–13.5)
Median bulb diam. 10.0 ± 0.8 (8–11)
Median bulb length/diam. 1.2 ± 0.1 (1.1–1.3)
Pharyngeal overlapping 40 ± 5 (31–49)
Maximum body diam. 16.0 ± 1.6 (13–20)
Vulval body diam. (VBD) 15.0 ± 1.4 (12–19)
Body diam. at median bulb 13.0 ±1.1 (11.5–15.0)
Postvulval uterine sac (PUS) 38 ± 8 (28–55)
PUS/VBD 2.5 ± 0.5 (2.0–3.7)
Vulva–anus 119 ± 13 (101–147)
Ovary length 161 ± 20 (130–193)
Anal body diam. 8.0 ± 0.9 (7–10)
Tail length 24.0 ± 1.4 (21–26)
eISSN:
2640-396X
Język:
Angielski
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Dziedziny czasopisma:
Life Sciences, other