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First Report of Bitylenchus hispaniensis, Pratylenchoides alkani, and Helicotylenchus vulgaris in Association with Cultivated and Wild Olives in Crete, Greece and Molecular Identification of Helicotylenchus microlobus and Merlinius brevidens

Emmanuel A. Tzortzakakis
Emmanuel A. Tzortzakakis
, 
Carolina Cantalapiedra-Navarrete
Carolina Cantalapiedra-Navarrete
, 
Maria Kormpi
Maria Kormpi
, 
Maria S. Lazanaki
Maria S. Lazanaki
, 
Pablo Castillo
Pablo Castillo
 oraz 
Antonio Archidona-Yuste
Antonio Archidona-Yuste
   | 17 paź 2018
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Data publikacji: 17 paź 2018
Zakres stron: 413 - 418
DOI: https://doi.org/10.21307/jofnem-2018-020
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© 2018 Emmanuel A. Tzortzakakis et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Geographic distribution of plant-parasitic nematodes of the genera Bitylenchus, Helicotylenchus, Merlinius and Pratylenchoides in field survey of cultivated and wild olive in Crete, Greece. Numbers of samples for each nematode species are as follows: Bitylenchus hispaniensis (seven samples), Helicotylenchus microlobus (three samples), Helicotylenchus vulgaris (seven samples), Merlinius brevidens (40 samples) and Pratylenchoides alkani (eight samples). 44 sites are presented, as some samples were derived from spatially close (encircled) areas and may therefore not be independent populations.
Geographic distribution of plant-parasitic nematodes of the genera Bitylenchus, Helicotylenchus, Merlinius and Pratylenchoides in field survey of cultivated and wild olive in Crete, Greece. Numbers of samples for each nematode species are as follows: Bitylenchus hispaniensis (seven samples), Helicotylenchus microlobus (three samples), Helicotylenchus vulgaris (seven samples), Merlinius brevidens (40 samples) and Pratylenchoides alkani (eight samples). 44 sites are presented, as some samples were derived from spatially close (encircled) areas and may therefore not be independent populations.

Sequenced genes pf plant-parasitic nematodes sampled from cultivated and wild olive in Crete and two other hosts in Evia and Thessaly, Greece.

Nematode species Sample code Host and Locality D2-D3 ITS
Bitylenchus hispaniensis OLI106 Cultivated olive, Episkopi, Crete MG770479 –
B. hispaniensis OLI013 Cultivated olive, Roufas, Crete * –
B. hispaniensis OLE011 Wild olive, Trypiti, Crete *
B. hispaniensis OLE015 Wild olive, Lentas, Crete * –
B. hispaniensis OLE023 Wild olive, Ag. Ioannis, Crete * –
B. hispaniensis OLE029 Wild olive, Ag. Georgios, Crete * –
B. hispaniensis OLE032 Wild olive, Tsoutsouros, Crete * –
Helicotylenchus microlobus OLI066 Cultivated olive, Vori, Crete MG770480 MG770406
H. microlobus OLI002 Cultivated olive, Vori, Crete * –
H. microlobus OLI074 Cultivated olive, Vori, Crete * –
H. microlobus POT017 Potted cultivated olive, Chania, Crete MG770481 MG770407
H. microlobus WAL17 Walnut, Evia, Greece MG770482 MG770408
Helicotylenchus vulgaris OLI004 Cultivated olive, Petrokefali, Crete MG770483 –
H. vulgaris OLI005 Cultivated olive, Petrokefali, Crete * –
H. vulgaris OLI046 Cultivated olive, Gonies, Crete * –
H. vulgaris OLI055 Cultivated olive, Voutes, Crete * –
H. vulgaris OLI071 Cultivated olive, Pentamodi, Crete * –
H. vulgaris OLI129 Cultivated olive, Perama, Crete * –
H. vulgaris OLE026 Wild olive, Agiofarago, Crete * –
H. vulgaris GOBERR Goji berry, Thessaly, Greece MG770484 –
Merlinius brevidens a OLI106 Cultivated olive, Episkopi, Crete MG770485 –
M. brevidens OLE009 Wild olive, Kefali, Crete * –
Pratylenchoides alkani b OLI060 Cultivated olive, Sivas, Crete MG770486 –
P. alkani OLI111 Cultivated olive, Amygdali, Crete * –
P. alkani OLE007 Wild olive, Vathy, Crete * –
P. alkani OLE017 Wild olive, Agiofarago, Crete * –
P. alkani OLE018 Wild olive, Agiofarago, Crete * –
P. alkani OLE031 Wild olive, Listis, Crete * –
P. alkani OLE032 Wild olive, Tsoutsouros, Crete * –
P. alkani OLE036 Wild olive, Agiofarago, Crete * –

Morphometrics of Bitylenchus hispaniensis (Handoo, et al., 2014); Helicotylenchus microlobus (Perry et al., 1959); Helicotylenchus vulgaris (Yuen, 1964); Merlinius brevidens (Allen, 1955; Siddiqi, 1970); and Pratylenchoides alkani (Yüksel, 1977) from olive in Crete, Greecea.

B. hispaniensis H. microlobus H. vulgaris M. brevidens P. alkani
Episkopi Vori Petrokefali Episkopi Sivas
Characters/ratiosb Females Females Females Females Females
n 3 4 3 3 3
L 722 ± 47 (670–760) 652 ± 31 (609–680) 672 ± 35 (634–702) 564 ± 116 (490–698) 907 ± 69.1 (840–978)
a 32.3 ± 0.6 (31.9–33.0) 25.4 ± 1.6 (24.2–27.7) 30.6 ± 0.4 (30.2–31.0) 26.3 ± 3.8 (22.9–30.3) 35.3 ± 0.8 (34.7–36.2)
B 6.3 ± 0.4 (6.1–6.8) 4.5 ± 0.4 (4.1–4.9) 5.1 ± 0.02 (5.1–5.2) 4.4 ± 0.6 (4.0–5.1) 6.7 ± 0.2 (6.4–6.8)
C 14.9 ± 0.6 (14.4–15.5) 43.7 ± 8.4 (35.8–55.4) 48.1 ± 1.1 (46.8–48.8) 14.3 ± 2.4 (12.9–17.0) 16.7 ± 0.2 (16.5–16.9)
c′ 3.0 ± 0.2 (2.7–3.2) 1.1 ± 0.2 (0.9–1.4) 1.1 ± 0.0 (1.1–1.1) 3.0 ± 0.1 (2.9–3.2) 3.1 ± 0.2 (2.8–3.2)
V 55.0 ± 1.0 (54.0–56.0) 61.2 ± 1.6 (59.3–63.2) 64.0 ± 2.0 (62.0–66.0) 54.7 ± 3.1 (52.0–58.0) 52.7 ± 1.5 (51.0–54.0)
Stylet length 17.3 ± 0.3 (17.0–17.5) 26.4 ± 1.2 (25.0–28.0) 24.0 ± 1.0 (23.0–25.0) 14.3 ± 1.5 (13.0–16.0) 23.7 ± 0.6 (23.0–24.0)
Pharynx length 114.0 ± 5.3 (110–120) 145.5 ± 7.0 (139–153) 130.7 ± 6.8 (123–136) 127.7 ± 7.2 (123–136) 135.7 ± 11.2 (123–144)
Max. body diam. 22.3 ± 1.2 (21.0–23.0) 25.7 ± 1.5 (24.0–27.0) 22.0 ± 1.0 (21.0–23.0) 21.3 ± 2.1 (19.0–23.0) 25.7 ± 1.5 (24.0–27.0)
Anal body diam. 16.5 ± 2.2 (14.0–18.0) 14.1 ± 1.4 (12.5–16.0) 13.0 ± 1.0 (12.0–14.0) 13.0 ± 1.0 (12.0–14.0) 17.7 ± 0.6 (17.0–18.0)
Tail 48.3 ± 3.1 (45.0–51.0) 15.3 ± 2.4 (12.0–17.0) 14.0 ± 1.0 (13.0–15.0) 39.3 ± 1.5 (38.0–41.0) 54.3 ± 3.5 (51.0–58.0)
eISSN:
2640-396X
Język:
Angielski
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Volume Open
Dziedziny czasopisma:
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