Species of genera
During a routine nematological survey of Hangzhou city, Zhejiang province, China, large populations of three criconematids were recovered from the rhizosphere of woody perennials. Morphological studies revealed the identity of these nematodes as
Thus, the objectives of the study were to: (1) establish the identity of these three species by morphological and molecular characterization, (2) integrate the morphometric characterization of Chinese populations of
Nematodes were also examined using a Hitachi TM-1000 scanning electron microscope (SEM). For the SEM examination, the nematodes were fixed in a mixture of 2.5% paraformaldehyde and 2.5% glutaraldehyde, washed three times in 0.1 M cocodylate buffer, post-fixed in 1% osmium tetroxide, dehydrated in a series of ethanol solutions and critical point-dried with CO2. After mounting on stubs, the samples were coated with gold. Specimens from Costa Rica were processed for SEM using the methods described in Powers et al. (2010).
DNA samples from China were prepared according to Zheng et al. (2003). Individual nematodes were transferred into an Eppendorf tube containing 16 µL ddH2O. Two microliters PCR buffer solution was added to each tube. Nematodes were crushed using a sterilized pipette tip, briefly spun and immediately frozen at −68oC for at least 30 min. The tubes were heated to 85oC for 2 min, briefly spun, followed by the addition of 2 µL proteinase K. The tubes were incubated at 56oC for 1 to 2 hrs, followed by 10 min at 95oC. After incubation, these tubes were cooled at 4oC and used for PCR (Zheng et al., 2003). Several sets of primers (synthesized by Invitrogen, Shanghai, China) were used in the PCR analyses to amplify the near full-length18S region of rDNA and COI region. Two sets of primers: the forward 18S39F (5′-AAA GAT TAA GCC ATG CAT G-3′) and the reverse 18S977R (5′-TTT ACG GTT AGA ACT AGG GCG G-3′), the forward 18S900F (5′-AAG ACG GAC TAC AGC GAA AG-3′) and the reverse 18S1713R (5′-TCA CCT ACA GCT ACC TTG TTA CG-3′) for amplification of the nearly full-length 18S rRNA (Olson et al., 2017). For the amplification of COI the primers used were COI-F5-(5′-AATWTWGGTGTTGGAACTTCTTGAAC-3′) and COI-R9-(5′ CTTAAAACATAATGRAAATGWGCWACWACATAATAAGTATC-3) (Powers et al., 2014). The 25-µl PCR was performed using 2x-TsingKe Master Mix DNA polymerase (Beijing TsingKe Biotech Co., Ltd) according to the manufacturer’s protocol in a BIOER-XP thermocycler. The thermal cycler program for 18S and COI was as follows: denaturation at 95°C for 5 min, followed by 40 cycles (18S) or 50 cycles (COI) of denaturation at 94°C for 30 s, annealing at 50°C (18S) or 48°C (COI) for 30 s, and extension at 72°C for 90 s. A final extension was performed at 72°C for 5 min as described by Powers et al. (2014) and Olson et al. (2017). PCR products were separated and visualized on 1% agarose gels and stained with ethidium bromide. PCR products of sufficiently high quality were sent for sequencing by Invitrogen (Shanghai, China).
Phylogenetic trees were constructed by maximum likelihood (ML) in MEGA version 6. Sequences were edited using CodonCode Aligner version 4.2 (
Morphometric data and distribution of
Authors | This study | Raski (1952) a | Loof (1991) | Eskandari et al. (2010) | Mirghasemi et al. (2014) | Rashid et al. (1986) | Popovivi and Ciobanu (2000) | Liskova et al. (2004) |
---|---|---|---|---|---|---|---|---|
Origin | Chinese population | Berkeley, California, USA | Iran | Iran | Iran | Brazil | Romania | Slovak Republic |
Host | Pomegranate |
|
|
- | Tea |
|
- |
|
n | 15 | - | 33 | 23 | 8 | 29 | 1 | 1 |
L | 270.4-324.5 | 259-295 | 240-330 | 252-313 | 260-346 | 210-270 | 299 | 280 |
a | 12.8-15.0 | 11.7-14.5 | 16-Oct | 8.8-13.5 | 11.3-14.4 | 12-Aug | 12.4 | 11.6 |
b | 3.7-4.2 | 3.0-3.4 | 3.2-4.2 | 3.2-4.1 | 3.1-4 | 3.0-3.6 | 3.9 | 3.3 |
c | 20.7-32.1 | - | 21-55 | 21.7-45.4 | 47.9-65 | 18-47 | 33.2 | 23.3 |
V | 93.6-95.6 | 92.5-95.9 | 94-97 | 93.6-96.7 | 95.9-96.5 | 91-94 | 95 | 94.3 |
VL/VB | 0.7-1.2 | - | 0.6-0.9 | 0.6-0.9 | 0.7-0.80 | 0.4-1.1 | 0.7 | 0.7 |
Stylet | 26.5-30.3 | 38-41 | 26-32 | 30-43 | 30.2-36.1 | 34.5-43 | 35 | 41 |
Stylet %L | 8.7-10.6 | - | 11-Sep | 10.4-15.9 | - | 14-18 | 12 | 14.6 |
R | 140.0-168 | 142-156 | 142-172 | 144-167 | 148-160 | 124-141 | 173 | 178 |
Rex | 43.0-48 | 46-49 | 41-53 | 45-53 | - | 39-52 | 50 | - |
RV | 9.0-13.0 | 11-12 | 8-12 | 9-13 | 8-10 | 8-11 | 12 | 16 |
RVan | 2.0-3 | - | 0-4 | 0-2 | 3-5 | 1-3 | 2 | 6 |
Ran | 6.0-10 | - | 6-11 | 7-11 | 5-7 | 6-9 | 9 | 10 |
Tail length | 9.1-15.6 | - | - | 6-14 | - | 6-11 | - | - |
Male | Unknown | Unknown | - | - | - | Known | - | - |
aOriginal description.
Systematics
Morphometrically, the three Iranian populations described by Loof and Barooti, (1991) have bodies that are slightly longer than the original description (240-346 µm
Morphometric data of
Authors | This study | Choi & Geraert, 1975 a |
---|---|---|
Origin | China | Korea |
Host |
|
|
n | 15 females | 5 females |
L | 333.1±19.0(307.9-382.6) | 285-315 |
a | 9.4±1.0(8.2-11.5) | 8.2-9.8 |
b | 2.5±0.1(2.3-2.8) | 2.5-2.6 |
c | 19.6±3.0(15.2-25.7) | - |
c' | 0.7±0.1(0.5-0.8) | - |
V | 89.2±0.8(87.8-90.4) | 87-90 |
VL/VB | 1.1±0.1(1.0-1.3) | - |
Stylet | 107.4±3.4(100.3-113.5) | 104-108 |
Stylet % L | 32.3±1.7(28.6-34.6) | - |
R | 91.3±2.2(88.0-94.0) | 82-90 |
Rex | 33.5±1.6(30.0-36.0) | 31 |
RV | 9.8±0.4(9.0-10.0) | 13-14 |
RVan | 5.2±0.8(4.0-6.0) | 7 |
Ran | 4.7±0.7(4.0-6.0) | 7 |
Lip height | 5.0±0.5(4.0-5.7) | - |
Pharynx | 131.5±3.3(126.4-137.5) | 121 |
Max. body diam. | 35.7±2.7(30.1-39.3) | 28 |
Vulva body diam. | 31.9±1.9(28.0-34.4) | - |
Dis. from vulva to tail term. | 35.9±3.2(31.3-43.3) | - |
Anal body diam. | 26.5±2.1(22.5-29.4) | - |
Tail length | 17.4±2.8(12.0-21.8) | 13 |
Light photomicrographs of
Scanning electron microscopy of
Light photomicrographs of
Scanning electron microscopy of
Morphometric data and distribution of
Authors | This study | Luc (1959) a | Coomans (1966) | Luc (1970) | Sauer and Winoto (1975) | Rashid et al. (1986) | Van den Berg and Cadet (1992) | Rahaman and Ahmed (1994) | Talavera and Hunt, 1997 | Crozzoli and Lamberti (2003) | Syn. |
Syn. |
|
|
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
origin | China | Costa Rica | French Guinea | Congo | Ivory Cost | Malaysia | Brazil | South Africa | India | Eucador | Venezuela | Brazil | Brazil | Peru |
Host |
|
Forest soil |
|
- |
|
Forest Soil |
|
Forest Soil |
|
Forest Soil | Forest Soil |
|
- | Forest Soil |
n | 15 | 5 | 3 | 8 | 20 | 11 | 36 | 11 | 20 | 18 | - | 10 | - | 8 |
L | 223.9- 270.1 | 225-270 | 207-228 | 260-280 | 180- 250 | 200-250 | 190-280 | 220-260 | 180-240 | 190-270 | 191-202 | 250-290 | 187-271 | 219-245 |
a | 5.9-7.5 | 6.8-8.7 | 5.8-7.6 | 8-11 | 7-8 | 5-8 | 7.5-9.5 | 5.9-7.4 | 6.2-6.7 | 7.6-12 | 5.6-6.4 | 7-8 | 6.8-8.8 | 8.3-9.5 |
b | 2.6-3.2 | 1.9-3.3 | 2.5-2.6 | 2.8-2.9 | 2.5-3 | 2.7-3.2 | 2.6-3.2 | 2.4-2.6 | 2.5-2.7 | 2.7-3.5 | 2.5-2.8 | 2.6-3.1 | - | 2.8-3.1 |
c | 15.7-24.7 | - | 16 | 17-25 | - | 20-33 | 20.4-46 | 17.8-22.3 | 14-21 | 15.5-20.5 | 15-20 | 20-26 | - | 27-39 |
V | 90.2-92.5 | 89-93 | 87-89 | 88-92 | 84-90 | 90-93 | 89-94 | 88-91 | 87-91 | 89.1-94.3 | 88-90 | 92-95 | - | 92-93 |
VL/VB | 0.8-1.2 | - | - | - | - | 0.6-1.1 | - | 0.87-1.1 | 0.8-1.4 | - | - | 0.8-0.9 | ||
Stylet | 53.1-59.6 | 45-57 | 52-53 | 53-55 | 38-50 | 45-53 | 43-52 | 52.5-59.2 | 48-51 | 35-51 | 48-54 | 59-66 | 43-56 | 44-47 |
Stylet %L | 20.2-25.3 | 18-25.3 | - | - | - | - | 17-23 | 22.3-25.2 | 23.5-26.6 | 16.6-19.7 | - | - | - | 18-20 |
R | 96.0-114 | 98-121 | 90-110 | 102-110 | 84-113 | 95-120 | 99-111 | 107-113 | 104-120 | 103-138 | 96-110 | 117-116 | 98-118 | 107-114 |
Rex | 33.0-37 | 33-41 | 34 | 32-36 | - | 35-38 | 34-44 | 28-33 | 34-39 | 37-47 | - | 37-42 | - | 30-34 |
RV | 11.0-13.0 | 10-11 | 11-12 | 12-15 | - | 11-15 | 8-14 | 12-15 | 14-16 | 9-12 | 12-14 | 10-11 | 9-13 | 11-13 |
RVan | 4.0-5.0 | - | 4 | 4-6 | - | 4-5 | 2-7 | 5-7 | 6-7 | 2-3 | 4-5 | 3-4 | 0-4 | |
Ran | 6.0-8.0 | - | 7 | 7-9 | - | 6-9 | 5-10 | 5-8 | 7-9 | 6-8 | 7-8 | 7-8 | 6-10 | 5-7 |
Tail | 9.8-16.4 | - | - | - | - | - | 4.5-13 | 10.3-14 | - | 10.5-16.5 | - | - | - | 5.6-8.9 |
Male | Unknown | Known | - | - | - | Known | - | - | Unknown | - | - | Known | - | - |
aOriginal description.
Morphometric data and distribution of
Authors | This study | Yin et al. (1994) | Ye et al. (1997) | Zhang et al. (1997) | Zhang et al. (1997) | Li et al. (2006) |
Origin (Province, City) | Zhejiang Hangzhou | Guangzhou | Guangdong | Fujian, Xiamen | Fujian, Zhang pu | Yunan, Kunming |
Host | Magnolia grandiflora |
|
|
Fruit trees | Fruit trees | Rosaceae plants |
n | 15 | 6 | 6 | 20 | 19 | - |
L | 223.9-270.1 | 225-260 | 217-280 | 180-230 | 210-260 | 183-257 |
a | 5.9-7.5 |
|
6.2-8.2 | 5.6-8.2 | 7-12 |
|
b | 2.6-3.2 |
|
2.3-3.4 | 2.3-3.3 | 206-301 |
|
c | 15.7-24.7 |
|
16.2-28.6 | 11-15 | 16-20 | |
V | 90.2-92.5 | 89-91 | 89-91 | 90-93 | 87-91 |
|
VL/VB | 0.8-1.2 |
|
094-1.06 |
|
|
|
Stylet | 53.1-59.6 | 51-53 | 46-56 | 51-57 | 45-50 |
|
Stylet % L | 20.2-25.3 |
|
|
|
|
|
R | 96.0-114 | 110-122 | 90-98 | 92-104 | 94-110 | 96-103 |
Rex | 33.0-37 |
|
30-34 | 30-37 | 30-36 | 34-37 |
RV | 11.0-13.0 | 10-12 | 11-13 | 8-10 | 10-13 | 12-14 |
RVan | 4.0-5.0 |
|
4-8 | 1-2 | 4-5 |
|
Ran | 6.0-8.0 |
|
5-8 | 5-8 | 6-9 |
|
Tail | 9.8-16.4 |
|
|
|
|
|
Male | Unknown |
|
|
|
Known |
|
Light photomicrographs of
Light photomicrographs of
Scanning electron microscopy of
Scanning electron microscopy of
Morphometrically, the Congo population (Coomans, 1966) is slightly longer than the original description (260-280 µm
Five additional populations of
Several key systematic features of criconematid nematodes are revealed by the 18S and COI phylogenetic trees. First, in the 18S tree (Fig. 9) which provides better resolution at the deeper nodes in the tree, there is strong bootstrap support (99%) for a clade that combines
Overall, the addition of these species from China to a reference dataset of criconematid nematodes provides insight into the biogeography of nematodes in general. It is likely that additional collections of plant parasitic nematodes from Asia will also contribute to fundamental questions of angiosperm biogeography (Raven and Axelrod, 1974; Fritsch et al., 2015; Wen et al., 2016).
Maximum likelihood tree of 232 18 S criconematid sequences. Substitution model GTR+G+I and 200 bootstrap replications. Each specimen is identified by a Nematode Identification number or GenBank Accession number (for taxa not sequenced by the authors), species name, and location as supplied by the author. Brackets are provided to indicate genera or specific species. Bootstrap values over 50 are applied by nodes in red. Specimens from China are highlighted in yellow.
Maximum likelihood COI tree of 175 criconematid sequences. Substitution model GTR+G+I and 200 bootstrap replications. Each specimen is identified by a Nematode Identification number, species name and location. Bootstrap values of more than 50 are applied by nodes in red. Specimens from China are highlighted in yellow.
Table of location data and GenBank accession numbers for specimens appearing on the COI maximum likelihood tree, in tree order.
NID | Species | Stage | Locality** | Ecoregion | GenBank Accession # |
---|---|---|---|---|---|
1057 |
|
J | Spring Creek Prairie, NE | Central Tall Grasslands | KJ788024 |
1129 |
|
F | Avoca Prairie and Savanna State Natural Area, WI | Upper Midwest Forest-Savanna Transition Zone | KJ788031 |
2973 |
|
F | Red Rock Prairie Preserve, MN | Central Tall Grasslands | KY574752 |
1054 |
|
F | Spring Creek Prairie, NE | Central Tall Grasslands | KJ788021 |
1388 |
|
F | Nine-Mile Prairie, NE | Central Tall Grasslands | KJ788053 |
1051 |
|
F | Stafford County, KS | Central and Southern Mixed Grasslands | KJ788019 |
5515 |
|
F | Downs Prairie Natural Area, AR | Mississippi Lowland Forests | KY574764 |
5501 |
|
J | Clymer Meadows, TX | Texas Blackland Prairies | KY574813 |
956 |
|
F | Schluckebier Prairie State Natural Area, WI | Upper Midwest Forest-Savanna Transition Zone | KJ788015 |
5502 |
|
F | Roth Prairie Natural Area, AR | Mississippi Lowland Forests | KY574731 |
1300 |
|
F | Lowndes County, MS | Southeastern Mixed Forests | KJ787926 |
5540 |
|
J | Chihuahuan Desert Research Institute, TX | Chihuahan Desert | MF770954 |
3660 |
|
F | Lance Rosier Unit, BITH, TX | Piney Woods Forests | KY574795 |
3086 |
|
F | Hayden Prairie Preserve, IA | Central Tall Grasslands | KY574679 |
5506 |
|
J | Roth Prairie Natural Area, AR | Mississippi Lowland Forests | KY574695 |
5505 |
|
F | Roth Prairie Natural Area, AR | Mississippi Lowland Forests | KY574724 |
5527 |
|
F | Warren Prairie Natural Area, AR | Piney Woods Forests | KY574807 |
1346 |
|
F | Great Falls Park, GWMP, VA | Southeastern Mixed Forests | KY574831 |
1375 |
|
F | Nine-Mile Prairie, NE | Central Tall Grasslands | KJ787916 |
2557 |
|
F | Autauga County, AL | Southeastern Mixed Forests | KY574633 |
3320 |
|
J | Twin Creeks, GRSM, TN | Appalachian-Blue Ridge Forests | KY574832 |
728 |
|
F | Pickens County, SC | Southeastern Mixed Forests | KJ787873 |
5587 |
|
F | Tuskegee National Forest, AL | Southeastern Mixed Forests | KY574626 |
5603 |
|
J | Big Sandy Creek Unit, BITH, TX | Piney Woods Forests | MF770959 |
2694 |
|
F | Albright Grove, GRSM, TN | Appalachian-Blue Ridge Forests | MF770909 |
3078 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | KY574639 |
3491 |
|
F | West Point, GRSM, TN | Appalachian-Blue Ridge Forests | MF770951 |
1135 |
|
F | Nine-Mile Prairie, NE | Central Tall Grasslands | KJ787935 |
7003 |
|
F | Prairie Pines, NE | Central Tall Grasslands | MF770967 |
2919 |
|
F | Aurora Prairie Preserve, SD | Central Tall Grasslands | KY574657 |
2920 |
|
J | Aurora Prairie Preserve, SD | Central Tall Grasslands | MF770921 |
3187 |
|
F | Roth Prairie Natural Area, AR | Mississippi Lowland Forests | KY574833 |
570 |
|
J | Jonathan Dickinson State Park, FL | Florida Sand Pine Scrub | KJ788061 |
3280 |
|
F | Apalachicola Bluffs and Ravines Preserve, FL | Southeastern Conifer Forests | KY574834 |
3646 |
|
F | Neches Bottom and Jack Gore Unit, BITH, TX | Piney Woods Forests | KY574817 |
2905 |
|
F | Barta Brothers Ranch, NE | Nebraska Sandhills Mixed Grasslands | KY574825 |
3175 |
|
F | Kellogg-Weaver Dunes SNA, MN | Central Tall Grasslands | KY574826 |
443 |
|
J | Brookings County, SD | Central Tall Grasslands | KJ787868 |
2627 |
|
F | Brushy Mtn., GRSM, TN | Appalachian-Blue Ridge Forests | KX290522 |
5976 |
|
J | Brushy Mtn., GRSM, TN | Appalachian-Blue Ridge Forests | KX290542 |
5990 |
|
F | Brushy Mtn., GRSM, TN | Appalachian-Blue Ridge Forests | KX290548 |
2900 |
|
F | Barta Brothers Ranch, NE | Nebraska Sandhills Mixed Grasslands | MF770919 |
2902 |
|
F | Barta Brothers Ranch, NE | Nebraska Sandhills Mixed Grasslands | MF770920 |
3966 |
|
F | Turkey Creek Unit, BITH, TX | Piney Woods Forests | MF770953 |
3085 |
|
F | Hayden Prairie Preserve, IA | Central Tall Grasslands | MF770940 |
5572 |
|
F | Akershus County, Norway | Scandinavian and Russian Taiga | KY574621 |
3050 |
|
F | Hayden Prairie Preserve, IA | Central Tall Grasslands | MF770936 |
3059 |
|
J | Hayden Prairie Preserve, IA | Central Tall Grasslands | MF770937 |
362 |
|
F | Treasure Co, MT | Northern Short Grasslands | KJ787847 |
3172 |
|
J | Kellogg-Weaver Dunes SNA, MN | Central Tall Grasslands | MF770942 |
3174 |
|
F | Kellogg-Weaver Dunes SNA, MN | Central Tall Grasslands | MF770943 |
3169 |
|
F | Kellogg-Weaver Dunes SNA, MN | Central Tall Grasslands | KY574821 |
3431 |
|
F | Oaky Woods Wildlife Management Area, GA | Southeastern Mixed Forests | KY574822 |
3457 |
|
F | Oaky Woods Wildlife Management Area, GA | Southeastern Mixed Forests | KY574823 |
3460 |
|
F | Oaky Woods Wildlife Management Area, GA | Southeastern Mixed Forests | MF770949 |
1242 |
|
F | Auburn University, Auburn, AL | Southeastern Mixed Forests | KJ787834 |
502 |
|
F | USDA Southeastern Fruit and Nut Tree Reseach Station, GA | Southeastern Mixed Forests | KJ787824 |
P184030 |
|
F | Las Cruces Biological Station, Costa Rica | Isthmian-Pacific Moist forests | KJ788069 |
ZB17051005279 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770975 |
ZB17042605146 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770973 |
ZB17051005280 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770976 |
ZB17042605147 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770974 |
ZB17051005282 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770978 |
ZB17051005281 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770977 |
1231 |
|
F | Pauchaug State Forest, WI | Northeastern Coastal Forests | MF770896 |
1460 |
|
F | Purchase Knob, GRSM, NC | Appalachian-Blue Ridge Forests | MF770902 |
1484 |
|
F | Arlington Woods, GWMP, VA | Southeastern Mixed Forests | MF770903 |
1157 |
|
F | Tippecanoe County, IN | Central Forest-Grassland Transition Zone | KU236522 |
3368 |
|
F | Crane Hollow Preserve, OH | Appalachian Mixed Mesophytic Forests | KU236539 |
2525 |
|
F | Michigan State University, East Lansing, MI | Southern Great Lakes Forests | KU236534 |
3382 |
|
F | Porcupine Mountains Wilderness State Park, MI | Western Great Lakes Forest | KU236626 |
3414 |
|
F | Parfrey's Glen State Natural Area, WI | Upper Midwest Forest-Savanna Transition Zone | KU236627 |
3317 |
|
F | Twin Creeks, GRSM, TN | Appalachian-Blue Ridge Forests | KU236521 |
3229 |
|
F | Ozark National Forest, AR | Central US Hardwood Forests | KU236631 |
5563 |
|
F | Emigration Canyon, UT | Wasatch and Uinta Montane Forests | KU236508 |
5576 |
|
J | Providence Canyon, Cache County, UT | Wasatch and Uinta Montane Forests | KU236620 |
5577 |
|
F | Providence Canyon, Cache County, UT | Wasatch and Uinta Montane Forests | KU236621 |
3675 |
|
F | Canyonlands, BITH, TX | Piney Woods Forests | KU236623 |
3677 |
|
F | Canyonlands, BITH, TX | Piney Woods Forests | KU236624 |
894 |
|
F | Chimney Creek, GRSM, TN | Appalachian-Blue Ridge Forests | KU236496 |
1206 |
|
F | Pine Cliff State Natural Area, WI | Upper Midwest Forest-Savanna Transition Zone | KU236491 |
3267 |
|
F | Ozark National Forest, AR | Central US Hardwood Forests | KU236495 |
3203 |
|
F | Ichetucknee Springs State Park, FL | Southeastern Conifer Forests | KU236554 |
577 |
|
F | Ichetucknee Springs State Park, FL | Southeastern Conifer Forests | KU236548 |
3195 |
|
F | Ichetucknee Springs State Park, FL | Southeastern Conifer Forests | KU236552 |
5583 |
|
F | St. Francis National Forest, AR | Mississippi Lowland Forests | MF770958 |
3645 |
|
F | Big Sandy Creek Unit, BITH, TX | Piney Woods Forests | KU236546 |
3668 |
|
F | Big Sandy Creek Unit, BITH, TX | Piney Woods Forests | KU236547 |
2914 |
|
F | Aurora Prairie Preserve, SD | Central Tall Grasslands | KU236570 |
1382 |
|
J | Spring Creek Prairie, NE | Central Tall Grasslands | KU236568 |
1 |
|
F | Timmas Farm State Ecological Preserve, NE | Central Tall Grasslands | KU236629 |
2273 |
|
F | Roy E. Larsen Sandyland Sanctuary, TX | Piney Woods Forests | KU236555 |
2862 |
|
F | Konza Prairie Biological Station, KS | Flint Hills Tall Grasslands | KU236557 |
3068 |
|
F | Cataloochee, GRSM, NC | Appalachian-Blue Ridge Forests | KU236601 |
3196 |
|
F | Ichetucknee Springs State Park, FL | Southeastern Conifer Forests | KU236613 |
3249 |
|
F | Oconaluftee, GRSM, NC | Appalachian-Blue Ridge Forests | KU236571 |
3257 |
|
F | Ozark National Forest, AR | Central US Hardwood Forests | KU236572 |
3057 |
|
F | Tunica Hills State Wildlife Refuge, LA | Mississippi Lowland Forests | KU236597 |
1395 |
|
F | Leiter Manxion & Turkey Run, GWMP, VA | Southeastern Mixed Forests | KU236630 |
3297 |
|
F | Torreya State Park, FL | Southeastern Conifer Forests | KU236608 |
3615 |
|
F | Big Sandy Creek Unit, BITH, TX | Piney Woods Forests | KU236588 |
3663 |
|
F | Lance Rosier Unit, BITH, TX | Piney Woods Forests | KU236590 |
1288 |
|
F | Spring Creek Prairie, NE | Central Tall Grasslands | MF770898 |
1455 |
|
F | Juan Diaz County, Puerto Rico | Puerto Rican Moist Forests | MF770901 |
ZB17051104893 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770968 |
1099 |
|
F | Custer Gallatin National Forest, MT | South Central Rockies Forests | MF77089 |
3669 |
|
F | Canyonlands Unit, BITH, TX | Piney Woods Forests | MF770952 |
5765 |
|
F | Roosevelt National Forest, CO | Colorado Rockies Forests | MF770961 |
5565 |
|
F | Emigration Canyon, UT | Wasatch and Uinta Montane Forests | MF770956 |
5566 |
|
F | Emigration Canyon, UT | Wasatch and Uinta Montane Forests | MF770957 |
1101 |
|
F | Gallatin National Forest, MT | South Central Rockies Forests | KJ787842 |
5788 |
|
J | Uncompahgre National Forest, CO | Colorado Rockies Forests | MF770962 |
2962 |
|
F | Hobe Sound National Wildlife Refuge, FL | Florida Sand Pine Scrub | MF770923 |
1261 |
|
F | Great Falls Park, GWMP, VA | Southeastern Mixed Forests | KJ788066 |
3040 |
|
J | Uinta-Wasatch-Cache National Forest, UT | Wasatch and Uinta Montane Forests | MF770934 |
3041 |
|
J | Uinta-Wasatch-Cache National Forest, UT | Wasatch and Uinta Montane Forests | MF770935 |
5921 |
|
J | Ontario, Canada | Eastern Great Lakes lowland forests | MF770965 |
5922 |
|
J | Ontario, Canada | Eastern Great Lakes lowland forests | MF770966 |
5748 |
|
F | Santa Fe National Forest, NM | Colorado Rockies Forests | MF770960 |
2508 |
|
F | Jepson Prairie Preserve, CA | California Central Valley Grasslands | MF770905 |
281 |
|
F | Hitchcock County, NE | Central and Southern Mixed Grasslands | MF770889 |
878 |
|
F | Steele County, ND | Northern Mixed Grasslands | MF770890 |
1328 |
|
F | Fresno County, CA | California Central Valley Grasslands | KU236637 |
3432 |
|
F | Oaky Woods Wildlife Management Area, GA | Southeastern Mixed Forests | MF770948 |
2460 |
|
F | Beaumont Unit, BITH, TX | Piney Woods Forests | MF770904 |
2686 |
|
F | Auburn University, Auburn, AL | Southeastern Mixed Forests | MF770908 |
1404 |
|
F | Leiter Mansion & Turkey Run, GWMP, VA | Southeastern Mixed Forests | MF770900 |
923 |
|
F | Laurel Falls Trail, GRSM, TN | Appalachian-Blue Ridge Forests | MF770891 |
927 |
|
F | Laurel Falls Trail, GRSM, TN | Appalachian-Blue Ridge Forests | MF770892 |
2766 |
|
F | Anderson County, SC | Southeastern Mixed Forests | MF770916 |
5545 |
|
F | Noh Bo Forest, Thailand | Kayah-Karen Montane Rain Forests | MF770955 |
ZB17042605150 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770969 |
ZB17051005283 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770970 |
ZB17051005284 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770971 |
ZB17051005285 |
|
F | Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF770972 |
3270 |
|
F | Noh Bo Forest, Thailand | Kayah-Karen Montane Rain Forests | MF770946 |
1212 |
|
F | Accotink Watershed, VA | Southeastern Mixed Forests | MF770894 |
1213 |
|
F | Accotink Watershed, VA | Southeastern Mixed Forests | MF770895 |
3141 |
|
F | Ponce de Leon State Park, FL | Southeastern Conifer Forests | MF770941 |
3483 |
|
F | Wakulla Springs State Park, FL | Southeastern Conifer Forests | MF770950 |
2991 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | MF770928 |
3356 |
|
F | Raspberry Island, APIS, WI | Western Great Lakes Forest | MF770947 |
2985 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | MF770926 |
3004 |
|
F | Goshen Prong, GRSM, TN | Appalachian-Blue Ridge Forests | MF770932 |
3005 |
|
F | Goshen Prong, GRSM, TN | Appalachian-Blue Ridge Forests | MF770933 |
1247 |
|
F | Nine-Mile Prairie, NE | Central Tall Grasslands | MF770897 |
1399 |
|
F | Nine-Mile Prairie, NE | Central Tall Grasslands | MF770899 |
2975 |
|
F | Red Rock Prairie Preserve, MN | Central Tall Grasslands | MF770924 |
2976 |
|
F | Red Rock Prairie Preserve, MN | Central Tall Grasslands | MF770925 |
2635 |
|
F | Brushy Mtn., GRSM, TN | Appalachian-Blue Ridge Forests | KX290563 |
2758 |
|
F | Trillium Gap, GRSM, TN | Appalachian-Blue Ridge Forests | MF770912 |
2759 |
|
F | Trillium Gap, GRSM, TN | Appalachian-Blue Ridge Forests | MF770913 |
2808 |
|
F | Twin Creeks, GRSM, TN | Appalachian-Blue Ridge Forests | MF770918 |
2807 |
|
J | Twin Creeks, GRSM, TN | Appalachian-Blue Ridge Forests | MF770917 |
3067 |
|
F | Cataloochee, GRSM, NC | Appalachian-Blue Ridge Forests | MF770938 |
3069 |
|
F | Cataloochee, GRSM, NC | Appalachian-Blue Ridge Forests | MF770939 |
3220 |
|
F | Purchase Knob, GRSM, NC | Appalachian-Blue Ridge Forests | MF770944 |
2724 |
|
J | Gregory Bald, GRSM, NC | Appalachian-Blue Ridge Forests | MF770910 |
2522 |
|
F | Michigan State University, East Lansing, MI | Southern Great Lakes Forests | MF770906 |
2993 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | KU236641 |
2994 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | MF770930 |
5842 |
|
F | Simes Tract, Harvard Forest LTER, MA | New England Acadian Forests | MF770963 |
5843 |
|
F | Simes Tract, Harvard Forest LTER, MA | New England Acadian Forests | MF770964 |
2995 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | MF770931 |
2755 |
|
F | Trillium Gap, GRSM, TN | Appalachian-Blue Ridge Forests | MF770911 |
2948 |
|
F | West Point, GRSM, TN | Appalachian-Blue Ridge Forests | MF770922 |
2761 |
|
F | Tongass National Forest, AK | Northern Pacific Coastal Forests | MF770914 |
2762 |
|
F | Tongass National Forest, AK | Northern Pacific Coastal Forests | MF770915 |
2989 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | MF770927 |
2992 |
|
F | Gifford Woods State Park, VT | New England Acadian Forests | MF770929 |
2573 |
|
F | Santa Cruz Island Reserve, CA | California Coastal Sage and Chaparral | MF770907 |
3262 |
|
F | Ozark National Forest, AR | Central US Hardwood Forests | MF770945 |
2636 |
|
F | Brushy Mtn., GRSM, TN | Appalachian-Blue Ridge Forests | KX290587 |
2753 |
|
F | Tongass National Forest, AK | Northern Pacific Coastal Forests | KX290594 |
2779 |
|
F | Tongass National Forest, AK | Northern Pacific Coastal Forests | KX290599 |
Table of location data and GenBank accession numbers for new
ZB17052504993 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795592 |
ZB17052504991 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795591 |
ZB17052504983 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795587 |
ZB17052504981 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795586 |
ZB17052504979 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795585 |
ZB17052504987 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795589 |
ZB17052504985 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795588 |
ZB17052504989 |
|
Hangzhou, Zhejiang Province, China | Eastern coast of China, flooded grasslands and savannas | MF795590 |