<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The incidence of positive autocontrol test results with column agglutination technology is a concern. This study investigates the incidence and significance of positive autocontrols in the ID Micro Typing System™ (gel) and the Gamma ReACT™ (ReACT). The study encompassed a total of 1021 randomly selected samples from patients and 95 samples from donors collected during 1 month. The autocontrol testing was carried out according to the manufacturer’s instructions for the column agglutination tests. The tube method was carried out using low-ionic-strength solution (USS). The direct antiglobulin test (DAT) was performed using the tube method, and further investigated with elution studies if warranted. Seventy-nine patient’s samples (7.74%) had a positive autocontrol: the gel test, 72 (91.13%); ReACT, 21 (26.58%); and the tube method, 27 (34.18%). Of the 79 positive autocontrols, 44 samples had a negative DAT. Of the samples with positive DAT results, only one possessed a clinically significant antibody, anti-D. Moreover, the same sample also tested positive in all three methods. Column agglutination techniques have increased sensitivity for a positive autocontrol beyond the conventional tube method. However, ReACT and gel tests differ significantly in their frequency of positives. Investigation of the significance of a positive autocontrol in column agglutination technology when the conventional tube method is also positive is suggested. <bold><italic>Immunobematology</italic> 2OOI;I7:5O—52</bold>.</p></abstract>

The incidence of positive autocontrol test results with column agglutination technology is a concern. This study investigates the incidence and significance of positive autocontrols in the ID Micro Typing System™ (gel) and the Gamma ReACT™ (ReACT). The study encompassed a total of 1021 randomly selected samples from patients and 95 samples from donors collected during 1 month. The autocontrol testing was carried out according to the manufacturer’s instructions for the column agglutination tests. The tube method was carried out using low-ionic-strength solution (USS). The direct antiglobulin test (DAT) was performed using the tube method, and further investigated with elution studies if warranted. Seventy-nine patient’s samples (7.74%) had a positive autocontrol: the gel test, 72 (91.13%); ReACT, 21 (26.58%); and the tube method, 27 (34.18%). Of the 79 positive autocontrols, 44 samples had a negative DAT. Of the samples with positive DAT results, only one possessed a clinically significant antibody, anti-D. Moreover, the same sample also tested positive in all three methods. Column agglutination techniques have increased sensitivity for a positive autocontrol beyond the conventional tube method. However, ReACT and gel tests differ significantly in their frequency of positives. Investigation of the significance of a positive autocontrol in column agglutination technology when the conventional tube method is also positive is suggested. Immunobematology 2OOI;I7:5O—52.

Tube and column agglutination technology for autocontrol testing

Immunohematology

{"article-title":"Tube and column agglutination technology for autocontrol testing"}

The incidence of positive autocontrol test results with column agglutination technology is a concern. This study investigates the incidence and significance of...

Tube and column agglutination technology for autocontrol testing

Data publikacji: 14 paź 2020

Zakres stron: 50 - 52

DOI: https://doi.org/10.21307/immunohematology-2019-544

Słowa kluczowe
autocontrol testing, ID Micro Typing System™, ReACT™

© 2019 J. E. Courtney et al., published by Sciendo

Tube and column agglutination technology for autocontrol testing

Data publikacji: 14 paź 2020

Zakres stron: 50 - 52

DOI: https://doi.org/10.21307/immunohematology-2019-544

Słowa kluczoweautocontrol testing, ID Micro Typing System™, ReACT™

© 2019 J. E. Courtney et al., published by Sciendo

Słowa kluczowe
autocontrol testing, ID Micro Typing System™, ReACT™