Indirect antiglobulin test-crossmatch using low-ionic-strength saline–albumin enhancement medium and reduced incubation time: effectiveness in the detection of most clinically significant antibodies and impact on blood utilization

1.Rumsey DH, Ciesielski DJ. New protocols in serologic testing: a review of techniques to meet today’s challenges. Immunohematology 2000;16:131–7.10.21307/immunohematology-2019-594Search in Google Scholar

2.Roback JD, Grossman BJ, Harris T, Hillyer CD. Technical manual. 17th ed. Bethesda, MD: American Association of Blood Banks, 2011.Search in Google Scholar

3.Jørgensen J, Nielsen M, Nielsen CB, Nørmark J. The influence of ionic strength, albumin and incubation time on the sensitivity of the indirect Coombs’ test. Vox Sang 1979;36:186–91.Search in Google Scholar

4.Skaik YA. Detection of Rh antibodies using two low ionic diluents: extension of the incubation time and the number of Rh antibodies detected. J Postgrad Med 2008;54:4–6.10.4103/0022-3859.3918118296796Search in Google Scholar

5.Reznicek MJ, Cordle DG, Strauss RG. A hemolytic reaction implicating Sda antibody missed by immediate spin crossmatch. Vox Sang 1992;62:173–5.Search in Google Scholar

6.Judd WJ, Steiner EA, Abruzzo LV, et al. Anti-i causing acute hemolysis following a negative immediate-spin crossmatch. Transfusion 1992;32:572–5.10.1046/j.1537-2995.1992.32692367204.x1502712Search in Google Scholar

7.Wise SC, Larson PJ, Cook LO. Antibodies to low-incidence antigens and elimination of the antihuman globulin phase of the crossmatch-case report: anti-Wr^a. Immunohematology 1997;13:20–2.10.21307/immunohematology-2019-696Search in Google Scholar

8.Koshy R, Patel B, Harrison JS. Anti-Kp^a-induced severe delayed hemolytic transfusion reaction. Immunohematology 2009;25:44–7.10.21307/immunohematology-2019-230Search in Google Scholar

9.Higgins JM, Sloan SR. Stochastic modeling of human RBC alloimmunization: evidence for a distinct population of immunologic responders. Blood 2008;112:2546–53.10.1182/blood-2008-03-14641518535200Search in Google Scholar

10.Judd WJ, Fullen DR, Steiner EA, Davenport RD, Knafl PC. Revisiting the issue: can the reading for serologic reactivity following 37 degrees C incubation be omitted? Transfusion 1999;39:295–9.10.1046/j.1537-2995.1999.39399219287.x10204593Search in Google Scholar

11.Chapman JF, Milkins C, Voak D. The computer crossmatch: a safe alternative to the serological crossmatch. Transfus Med 2000;10:251–6.10.1046/j.1365-3148.2000.00274.x11123808Search in Google Scholar