<abstract xmlns="http://www.w3.org/1999/xhtml"> Introduction. Abnormal angiogenesis is described in tumor growth and it facilitates its metastatic spread. Tumors with high angiogenic activity belong to the category of aggressive tumors with poor prognosis for patients.The aim of this study was to determine the blood vessels density (BVD), i.e. neovascularization at the tumor invasive front in skin squamous cell carcinoma (SCC) in order to determine its possible role in the tumor progression, and to correlate it to the blood vessels density of healthy skin and with the prognostic parameters of the TNM classification: T status, depth of tumor invasion (DI) and tumor histological grade (G), which were also correlated between each other.Material and Methods. The material consisted of surgical specimens obtained from 30 patients with skin SCC, who underwent surgery.Tissue samples were routinely processed by standard paraffin technique stained by Hematoxilin-Eosin and immunohistochemically with antibodies against smooth muscle actin (SMA) and CD34. The BVD in the invasive front of the neoplasms was correlated to the healthy skin, tumor status (pT), depth of invasion and grade of histological differentiation (pG).Results. The histological analysis has shown a high statistical difference in the density of blood vessels in SCC compared to the healthy skin and statistical difference in BVD in neoplasms with different depth of invasion and different grade of differentiation. The density of neovascularzation increased with the deeper invasion and the worse differentiation.Conclusion. The increased vascularization at the invasive front of SCC with deeper invasion and worse differentiation has pointed out to its possible role in neoplasm progression.</abstract>

Introduction. Abnormal angiogenesis is described in tumor growth and it facilitates its metastatic spread. Tumors with high angiogenic activity belong to the category of aggressive tumors with poor prognosis for patients.The aim of this study was to determine the blood vessels density (BVD), i.e. neovascularization at the tumor invasive front in skin squamous cell carcinoma (SCC) in order to determine its possible role in the tumor progression, and to correlate it to the blood vessels density of healthy skin and with the prognostic parameters of the TNM classification: T status, depth of tumor invasion (DI) and tumor histological grade (G), which were also correlated between each other.Material and Methods. The material consisted of surgical specimens obtained from 30 patients with skin SCC, who underwent surgery.Tissue samples were routinely processed by standard paraffin technique stained by Hematoxilin-Eosin and immunohistochemically with antibodies against smooth muscle actin (SMA) and CD34. The BVD in the invasive front of the neoplasms was correlated to the healthy skin, tumor status (pT), depth of invasion and grade of histological differentiation (pG).Results. The histological analysis has shown a high statistical difference in the density of blood vessels in SCC compared to the healthy skin and statistical difference in BVD in neoplasms with different depth of invasion and different grade of differentiation. The density of neovascularzation increased with the deeper invasion and the worse differentiation.Conclusion. The increased vascularization at the invasive front of SCC with deeper invasion and worse differentiation has pointed out to its possible role in neoplasm progression.

Correlation Between Microvessel Density and Morphological Features in Skin Squamous Cell Carcinoma

Institute of Medical, Experimental and Applied Histology and Embryology, Faculty of Medicine, “Ss. Cyril and Methodius” University, Skopje,

Macedonia (the former Yugoslav Republic of)

Department of Histopathology and Clinical Cytology, University Clinic of Radiotherapy and Oncology, Faculty of Medicine, “Ss. Cyril and Methodius” University, Skopje,

University Clinic of Radiotherapy and Oncology, Faculty of Medicine, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia, Institute for radiotherapy and oncology, Skopje,

Institute of Pathology, Faculty of Medicine, “Ss. Cyril and Methodius” University, Skopje,

Macedonian Academy of Sciences and Arts, Skopje,

PRILOZI

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Introduction. Abnormal angiogenesis is described in tumor growth and it facilitates its metastatic spread. Tumors with high angiogenic activity belong to the...

Correlation Between Microvessel Density and Morphological Features in Skin Squamous Cell Carcinoma

Data publikacji: 20 kwi 2017

Zakres stron: 63 - 73

DOI: https://doi.org/10.1515/prilozi-2017-0009

Słowa kluczowe
squamous cell carcinoma, immunohistochemistry, CD34, density of neovascularization, invasive front, histological differentiation

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Correlation Between Microvessel Density and Morphological Features in Skin Squamous Cell Carcinoma

Data publikacji: 20 kwi 2017

Zakres stron: 63 - 73

DOI: https://doi.org/10.1515/prilozi-2017-0009

Słowa kluczowesquamous cell carcinoma, immunohistochemistry, CD34, density of neovascularization, invasive front, histological differentiation

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Słowa kluczowe
squamous cell carcinoma, immunohistochemistry, CD34, density of neovascularization, invasive front, histological differentiation