[Agencia Nacional de Vigilância Sanitária. (2010). Resolução-RDC nº 14, de 31 de março de 2010. Brasília, Brasil.]Search in Google Scholar
[Agencia Nacional de Vigilância Sanitária. (2004). Resolução nº 90, de 16 de março de 2004. Brasília, Brasil.]Search in Google Scholar
[Akerreta S, Cavero RY, Calvo MI. (2007). First comprehesive contribution to medical ethnobotany of wester pyrenes. J Ethnobiol Ethnomed, 3 26.10.1186/1746-4269-3-26]Search in Google Scholar
[Arechabala B, Coiffard C, Rivalland P, Coiffard LJ, de Roeck-Holtzhauer Y. (1999). Comparison of cytotoxicity of various surfactants tested on normal human fi broblast cultures using the neutral red test, MTT assay and LDH release. J Appl Toxicol, 19, 163-65.10.1002/(SICI)1099-1263(199905/06)19:3<163::AID-JAT561>3.0.CO;2-H]Search in Google Scholar
[Bent S. (2008). Herbal medicine in the United States: review of effi cacy, safety, and regulation: grand rounds at University of California, San Francisco Medical Center. J Gen Intern Med, 23, 854-59.10.1007/s11606-008-0632-y]Search in Google Scholar
[Berridge MV, Herst PM, Tan AS. (2005). Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev, 11, 127-52.10.1016/S1387-2656(05)11004-7]Search in Google Scholar
[Borenfreund E, Puerner JA. (1985). Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett, 24, 119-24.10.1016/0378-4274(85)90046-3]Search in Google Scholar
[Borenfreund E, Babich H, Martin-Alguacil N. (1988). Comparisons of two in vitro cytotoxicity assays-the neutral red (nr) and tetrazolium mtt tests. Toxicol In Vitro, 2, 1-6.10.1016/0887-2333(88)90030-6]Search in Google Scholar
[Bruggisser R, von Daeniken K, Jundt G, Schaffner W, Tullberg-Reinert H. (2002). Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta Medica, 68, 445-8.10.1055/s-2002-32073]Search in Google Scholar
[Bunel V, Ouedraogo M, Nguyen AT, Stévigny C, Duez P. (2014). Methods applied to the in vitro primary toxicology testing of natural products: State of the art, strengths, and limits. Planta Med [Epub in print]10.1055/s-0033-1360273]Search in Google Scholar
[Butler M. (2004). Animal cell culture and technology. 2a Ed. London, England: Taylor & Francis.10.4324/9780203427835]Search in Google Scholar
[Česen MH, Pegan K, Spes A, Turk B. (2012). Lysosomal pathways to cell death and their therapeutic applications. Exp Cell Res, 318, 1245-51.10.1016/j.yexcr.2012.03.005]Search in Google Scholar
[Fotakis G, Timbrell JA. (2006). In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett, 160, 171-7.10.1016/j.toxlet.2005.07.001]Search in Google Scholar
[Freshney RI. (2005). Culture of animal cells: a manual of basic technique. 5a. Ed. New Jersey, U.S.: Wiley-Blackwell.]Search in Google Scholar
[Khatibisepehr S, Huang B, Ibrahim F, Xing JZ, Roa W. (2011). Data-based modeling and prediction of cytotoxicity induced by contaminants in water resources. Comput Biol Chem, 35, 69-80.10.1016/j.compbiolchem.2011.02.001]Search in Google Scholar
[Kim H, Yoon SC, Lee TY, Jeong D. (2009). Discriminative cytotoxicity assessment based on various cellular damages. Toxicol Lett, 184, 13-7.10.1016/j.toxlet.2008.10.006]Search in Google Scholar
[Lupu AR, Popescu T. (2013). The noncellular reduction of MTT tetrazolium salt by TiO2 nanoparticles and its implications for cytotoxicity assays. Toxicol In Vitro, 27, 1445-50.10.1016/j.tiv.2013.03.006]Search in Google Scholar
[Malich G, Markovic B, Winder C. (1997). The sensitivity and specifi city of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines. Toxicology, 124, 179-92.10.1016/S0300-483X(97)00151-0]Search in Google Scholar
[Mosmann T. (1983). Rapid colorimetric assays for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65, 55-63.10.1016/0022-1759(83)90303-4]Search in Google Scholar
[National Institutes of Health. (2006). Background Review Document (BRD): Validation of Neutral Red Uptake Test Methods NIH/In Vitro Cytotoxicity Test Methods for Estimating Acute Oral Systemic Toxicity. Publication No. 07-4518, November 2006. Available at: http://ntp.niehs.nih.gov/iccvam/docs/acutetox_docs/BRD_TMER/BRDvol1_Nov2006.pdf Accessed on 16 April 2014.]Search in Google Scholar
[National Institutes of Health. (2001). Report of the International Workshop on In Vitro Methods for Assessing Acute Systemic Toxicity. NIH Publication No. 01-4499. National Institute for Environmental Health Sciences, Research Triangle Park, NC. Available at: http://www.epa.gov/hpv/pubs/general/nih2001a.pdf Accessed on 16 April 2014.]Search in Google Scholar
[Peng L, Wang B, Ren P. (2005). Reduction of MTT by fl avonoids in the absence of cells. Colloids Surf B Biointerfaces, 45, 108-11.10.1016/j.colsurfb.2005.07.01416150580]Search in Google Scholar
[Piersma AH. (2006). Alternative Methods for Developmental Toxicity Testing. Basic Clin Pharmacol Toxicol, 98, 427-31.10.1111/j.1742-7843.2006.pto_373.x16635099]Search in Google Scholar
[Repetto G, del Peso A, Zurita JL. (2008). Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc, 3, 1125-31.10.1038/nprot.2008.7518600217]Search in Google Scholar
[Rogero SO, Lugão AB, Ikeda TI, Cruz AS. (2003). Teste in vitro de cytotoxicidade: estudo comparativo entre duas metodologias. Mat Res, 6, 317-20.10.1590/S1516-14392003000300003]Search in Google Scholar
[Shoemaker M, Cohen I, Campbell M. (2004). Reduction of MTT by aqueous herbal extracts in the absence of cells. J Ethnopharmacol, 93, 381-4.10.1016/j.jep.2004.04.011]Search in Google Scholar
[Suares Rocha P, Azab E, Schmidt B, Storch V, Hollert H, Braunbeck T. (2010). Changes in toxicity and dioxin-like activity of sediments from the Tiete River (Sao Paulo, Brazil). Ecotoxicol Environ Saf, 73, 550-8.10.1016/j.ecoenv.2009.12.017]Search in Google Scholar
[Thode VA, Mentz LA. (2010). The genus Glandularia J.F. Gmel. (Verbenaceae) in Rio Grande do Sul, Brazil. Acta Bot Bras, 24. [On line]. Available at: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000200023. Accessed on 13 Aug 2014.]Search in Google Scholar
[Triglia D, Sherard Braa S, Yonan C, Naughton GK. (1991). Cytotoxicity testing using neutral red and mtt assays on a three- dimensional human skin substrate. Toxicol In Vitro, 5, 573-8.10.1016/0887-2333(91)90096-V]Search in Google Scholar
[Trintinaglia L, Bianchi E, Basso da Silva L, Nascimento CA, Spilki FR, Ziulkoski AL. 2015.Cytotoxicity assays as tools to assess water quality in the Sinos river basin. Braz J Biol, 75, 2 suppl, 75-80.]Search in Google Scholar
[U.S. Congress Office of Technology Assessment. (1986). Alternatives to Animal Use in Research, Testing, and Education. Washington, DC, U.S: Government Printing Offi ce.]Search in Google Scholar
[Vanden Berghe T, Grootjans S, Goossens V, Dondelinger Y, Krysko DV, Takahashi N, Vandenabeele P. (2013). Determination of apoptotic and necrotic cell death in vitro and in vivo. Methods, 61, 117-29.10.1016/j.ymeth.2013.02.011]Search in Google Scholar
[Von Poser GL, Toff oli ME, Sobral M, Henriques AT. (1997). Iridoid glucosides substitution patterns in Verbenaceae and their taxonomic implication. Plant Syst Evol, 205, 265-87.10.1007/BF01464409]Search in Google Scholar
[World Health Organization. (2005). National policy on traditional medicine and regulation of herbal medicines. Geneva, Switzerland: WHO Press.]Search in Google Scholar
[World Health Organization. (2013). WHO traditional medicine strategy: 2014-2023. Geneva, Switzerland: WHO Press.]Search in Google Scholar
[Zeni ALB, Zombowski ADE, Dal-Cim T, Maraschin M, Rodrigues ALS, Tasca CI. (2011). Antidepressant-like and neuroprotective eff ects of Aloysia gratissima: Investigation of involvement of L-arginina-nitric oxide-cyclic guanoside monophosphate pathway. J Ethnopharmacol, 37, 864-74.10.1016/j.jep.2011.07.009]Search in Google Scholar
[Zhang SZ, Lipsky MM, Trump BF, Hsu IC. (1990). Neutral red (NR) assay for cell viability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes. Cell Biology and Toxicology, 6, 219-34.10.1007/BF00249595]Search in Google Scholar
[Zucco F, De Angelis I, Testai E, Stammati A. (2004). Toxicology investigations with cell culture systems: 20 years after. Toxicol In Vitro, 18, 153-63.10.1016/S0887-2333(03)00147-4]Search in Google Scholar