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Characterisation of Trichodorus similis (Nematoda: Trichodoridae) associated with potato from the Czech Republic


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Fig. 1

Photomicrographs of Trichodorus similisSeinhorst, 1963, female and male.A – C: Anterior region of female (A, B) and male (C); D: Female reproductive system (anterior genital branch); E, F: Posterior body region (male tail and spicules); G: Male gonad; H: Vagina.
Photomicrographs of Trichodorus similisSeinhorst, 1963, female and male.A – C: Anterior region of female (A, B) and male (C); D: Female reproductive system (anterior genital branch); E, F: Posterior body region (male tail and spicules); G: Male gonad; H: Vagina.

Fig. 2

Photomicrographs of Trichodorus similisSeinhorst, 1963, female and male.A: Lips, onchiostyle and ventromedian pores of male; B: Lip and onchiostyle region (female); C: Amphid; D, E – Variations in vagina; F: Male tail and spicules; G, H: Variations in female tail.
Photomicrographs of Trichodorus similisSeinhorst, 1963, female and male.A: Lips, onchiostyle and ventromedian pores of male; B: Lip and onchiostyle region (female); C: Amphid; D, E – Variations in vagina; F: Male tail and spicules; G, H: Variations in female tail.

Fig. 3

Electrophoresis of the amplified products of Trichodorus similis: lane M – 100bp DNA ladder (Fermentas); lane 1 – female1; lane 2 – female2; lane 3 – T. pakistanensis; lane 4 – T. variopapillatus 4; lane 5 – T. viruliferus and lane 6: negative control.
Electrophoresis of the amplified products of Trichodorus similis: lane M – 100bp DNA ladder (Fermentas); lane 1 – female1; lane 2 – female2; lane 3 – T. pakistanensis; lane 4 – T. variopapillatus 4; lane 5 – T. viruliferus and lane 6: negative control.

Morphometrics of Trichodorus similis females from Kaliště region. Measurements in μm.

CharacterMean ± SD (range)n
L758 ± 63 (630 – 863)25
Onchiostyle39.3 ± 0.84 (38 – 42)25
Pharyngostom45.3 ± 1.84 (42 – 49)25
Pharynx138.7 ± 7.81 121 – 152)25
Anterior to guide ring19.0 ± 0.70 (18 – 20)21
Anterior to nerve ring63.4 ± 3.1 (59 – 66)22
Anterior to SE86.9 ± 5.07 (81 – 98)8
Body diam. at cardia27.4 ± 1.52 (24 – 30)25
Mid-body diam.30.8 ± 2.06 (26 – 34)25
Length anterior genital branch140.6 ± 9.23 (129 – 156)6
Length posterior genital branch134.9 ± 8.29 (125 – 152)6
a24.7 ± 1.7 (21.3 – 28.1)25
b5.5 ± 0.4 (4.7 – 6.3)25
V55.8 ± 2.5 (50.2 – 62.3)25
G117.8 ± 1.6 (15.4 – 19.9)6
G217.2 ± 2.0 (15.6 – 21.4)6

Morphometrics of Trichodorus similis males from Kaliště region. Measurements in μm.

CharacterMean ± SD (range)n
L730 ± 44 (658 – 783)6
Onchiostyle38.9 ± 22 (37 – 40)5
Pharyngostom44 ± 0.6 (43 – 45)6
Pharynx136.7 ± 2.9 (132 – 142)6
Anterior to guide ring18.4 ± 0.6 (17.5 – 19)5
Anterior to nerve ring59.7 ± 2 (56 – 61)6
Anterior to SE77.0 ± 6.9 (71 – 85)5
Anterior to CP132.3 ± 2.9 (29.5 – 37)5
Distance CP1 to CP220.2 ± 2 (17 – 22)5
Distance CP2 to CP314.8 ± 2.2 (11 – 16)5
Distances CP3 to SE11 ± 5 (7.5 – 20)3
Distance CP1 to SE46 ± 4 (42 – 50.5)3
Body diam. at cardia26 ± 1.6 (23 – 28)6
Mid – body diam.28.2 ± 2.5 (25 – 31)6
Anal body diam.23.5 ± 2.1 (20 – 26.5)6
Spicule length36.6 ± 1 (34 – 38)6
Gubernaculum15.6 ± 0.5 (15 – 16)5
Distance SP1 to cloacal opening26.7 ± 1.2 (25 – 28)6
Distance SP1 to SP229.2 ± 2.5 (25 – 32)6
Distance SP2 to SP340.2 ± 5.7 (35.5 – 52)6
Tail15.2 ± 1.2 (13 – 16.5)6
a26.0 ± 1.9 (23.6 – 29.0)6
b5.3 ± 0.30 (4.8 – 5.7)6
c48.2 ± 3.2 (44.1 – 53.2)6
c'0.6 ± 0.03 (0.6 – 0.7)6
eISSN:
1336-9083
ISSN:
0440-6605
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Zoology, Ecology, other, Medicine, Clinical Medicine, Microbiology, Virology and Infection Epidemiology