<abstract xmlns="http://www.w3.org/1999/xhtml">In this study sol-gel hybrid materials in the system SiO2-chitosan (CS) - polyethylene glycol (PEG), as novel structures with potential application in bioremediation were investigated. The organic components - CS and PEG were used as structural modifiers for functionality improvement. The catabolic activity to n-hexadecane of <italic>Pseudomonas aeruginosa</italic> BN10 free and immobilized cells was estimated. The cell immobilization technique was employed to evaluate its efficiency on biodegradation and protective effect from high levels of hydrocarbons. The characteristics of obtained hybrid materials were investigated via <italic>X-ray Diffraction</italic> (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and <italic>Atomic-force microscopy</italic> (AFM) analyses. The obtained results revealed that the organic part in the synthesized hybrids is important for microstructure and defined properties creation. The rate of n-hexadecane mineralization by the bacterial strain was influenced by variation in cell densities applied in the immobilization procedures. Semi-continuous processes with multiple xenobiotic supplies were carried out. The synthesized by the sol-gel method hybrid matrices proved to be suitable carriers for realizing an effective biodegradation process of n-hexadecane by <italic>Pseudomonas aeruginosa</italic> BN10. Biodegradation of 50 kg/m3 of n-hexadecane was realized by free cells. Significantly greater quantity (150 kg/m3) was mineralized for 15 active cycles by entrapped bacterial cells. Biodegradation process with gradual increase of xenobiotic concentration reaching 30 kg/m3 for 120 h was also accomplished.</abstract>

In this study sol-gel hybrid materials in the system SiO2-chitosan (CS) - polyethylene glycol (PEG), as novel structures with potential application in bioremediation were investigated. The organic components - CS and PEG were used as structural modifiers for functionality improvement. The catabolic activity to n-hexadecane of Pseudomonas aeruginosa BN10 free and immobilized cells was estimated. The cell immobilization technique was employed to evaluate its efficiency on biodegradation and protective effect from high levels of hydrocarbons. The characteristics of obtained hybrid materials were investigated via X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Atomic-force microscopy (AFM) analyses. The obtained results revealed that the organic part in the synthesized hybrids is important for microstructure and defined properties creation. The rate of n-hexadecane mineralization by the bacterial strain was influenced by variation in cell densities applied in the immobilization procedures. Semi-continuous processes with multiple xenobiotic supplies were carried out. The synthesized by the sol-gel method hybrid matrices proved to be suitable carriers for realizing an effective biodegradation process of n-hexadecane by Pseudomonas aeruginosa BN10. Biodegradation of 50 kg/m3 of n-hexadecane was realized by free cells. Significantly greater quantity (150 kg/m3) was mineralized for 15 active cycles by entrapped bacterial cells. Biodegradation process with gradual increase of xenobiotic concentration reaching 30 kg/m3 for 120 h was also accomplished.

New Approach for n-Hexadecane Biodegradation by Sol-Gel Entrapped Bacterial Cells

University of Chemical Technology and Metallurgy

Institute of Microbiology “Stephan Angeloff”, Bulgarian Academy of Sciences

Ecological Chemistry and Engineering S

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In this study sol-gel hybrid materials in the system SiO2-chitosan (CS) - polyethylene glycol (PEG), as novel structures with potential application in...

New Approach for n-Hexadecane Biodegradation by Sol-Gel Entrapped Bacterial Cells

Data publikacji: 17 lip 2018

Zakres stron: 243 - 253

DOI: https://doi.org/10.1515/eces-2018-0016

Słowa kluczowe
biodegradation, n-hexadecane, hybrids, sol-gel,

© 2018 Georgi Chernev et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

New Approach for n-Hexadecane Biodegradation by Sol-Gel Entrapped Bacterial Cells

Data publikacji: 17 lip 2018

Zakres stron: 243 - 253

DOI: https://doi.org/10.1515/eces-2018-0016

Słowa kluczowebiodegradation, n-hexadecane, hybrids, sol-gel,

© 2018 Georgi Chernev et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
biodegradation, n-hexadecane, hybrids, sol-gel,