Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113

The effects of the carbon and nitrogen substrates on the growth of Bacillus sp. SG113 strain were studied. The use of organic nitrogen sources (peptone, beef extract, yeast extract, casein) leads to rapid cellular growth and the best results for the Bacillus strain were obtained with casein hydrolysate. From the inorganic nitrogen sources studied, the (NH₄) ₂SO₄ proved to be the best nitrogen source. Casein hydrolysate and (NH₄) ₂SO₄ stimulated the invertase synthesis. In the presence of Jerusalem artichoke, onion and garlic extracts as carbon sources the strain synthesized from 6 to 10 times more inulinase.