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Evaluation of various phenotypic methods with genotypic screening for detection of methicillin-resistant Staphylococcus aureus


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Figure 1

The percentage of resistance (dark-gray bars) and susceptibility (light-gray bars) observed for 13 antibiotics by disk diffusion test
The percentage of resistance (dark-gray bars) and susceptibility (light-gray bars) observed for 13 antibiotics by disk diffusion test

Figure 2

Receiver operating characteristics (ROCs) for (A) cefoxitin disk diffusion (CFD), (B) minimum inhibitory concentration (MIC), (C) oxacillin disk diffusion (OD), and (D) oxacillin screen agar (OSA) illustrate area under the curve (AUC) statistics
Receiver operating characteristics (ROCs) for (A) cefoxitin disk diffusion (CFD), (B) minimum inhibitory concentration (MIC), (C) oxacillin disk diffusion (OD), and (D) oxacillin screen agar (OSA) illustrate area under the curve (AUC) statistics

Result of genotypic and phenotypic methods of 50 S. aureus

Isolate no.mecA geneOSA testOxacillin MICCefoxitin disk testOxacillin diskβ-Lactamase test
SA1+Growth128 (R)ResistantResistantPositive
SA2No growth1 (S)SusceptibleSusceptibleNegative
SA3+Growth>128 (R)ResistantResistantPositive
SA4+Growth64 (R)ResistantResistantPositive
SA5+No growth8 (R)ResistantResistantPositive
SA6No growth8 (R)SusceptibleResistantPositive
SA7+Growth>128 (R)ResistantResistantPositive
SA8Growth64 (R)ResistantResistantPositive
SA9+Growth128 (R)ResistantResistantPositive
SA10+Growth36 (R)ResistantResistantPositive
SA11+Growth>128 (R)ResistantResistantPositive
SA12+No growth128 (R)ResistantResistantPositive
SA13+Growth>128 (R)ResistantResistantPositive
SA14+Growth>128 (R)ResistantResistantPositive
SA15+Growth4 (R)ResistantSusceptiblePositive
SA16+Growth8 (R)ResistantSusceptiblePositive
SA17+Growth64 (R)ResistantResistantPositive
SA18+Growth>128 (R)ResistantResistantPositive
SA19No growth32 (R)ResistantResistantPositive
SA20Growth>128 (R)ResistantResistantPositive
SA21+Growth>128 (R)ResistantResistantPositive
SA22Growth8 (R)SusceptibleResistantPositive
SA23Growth>128 (R)ResistantResistantPositive
SA24+Growth>128 (R)ResistantResistantPositive
SA25No growth1 (S)SusceptibleSusceptiblePositive
SA26+Growth>128 (R)ResistantResistantPositive
SA27No growth1 (S)SusceptibleResistantPositive
SA28Growth1 (S)SusceptibleSusceptiblePositive
SA29Growth>128 (R)ResistantSusceptiblePositive
SA30No growth64 (R)SusceptibleSusceptiblePositive
SA31Growth>128 (R)ResistantResistantPositive
SA32Growth32 (R)ResistantResistantPositive
SA33Growth64 (R)ResistantResistantPositive
SA34Growth2 (S)SusceptibleSusceptiblePositive
SA35No growth1 (S)SusceptibleSusceptiblePositive
SA36No growth0.5 (S)SusceptibleSusceptiblePositive
SA37+Growth8 (R)ResistantResistantPositive
SA38No growth1 (S)SusceptibleSusceptiblePositive
SA39Growth64 (R)ResistantResistantPositive
SA40+No growth16 (R)ResistantSusceptiblePositive
SA41No growth2 (S)SusceptibleSusceptiblePositive
SA42No growth1 (S)SusceptibleSusceptiblePositive
SA43Growth1 (S)SusceptibleSusceptiblePositive
SA44No growth0.5 (S)SusceptibleSusceptiblePositive
SA45Growth16 (R)ResistantResistantPositive
SA46Growth8 (R)ResistantResistantPositive
SA47No growth0.5 (S)SusceptibleSusceptiblePositive
SA48+Growth8 (R)ResistantResistantPositive
SA49No growth2 (S)SusceptibleSusceptiblePositive
SA50Growth2 (S)SusceptibleResistantPositive

Correlation coefficient by Pearson's correlation coefficient method for comparison of phenotypic methods

Phenotypic methods in combinationsPearson's rank correlation coefficient (r)
MIC+CFD0.8729
MIC+OD0.6910
OD+CFD0.6528
CFD+OSA0.6052
MIC+OSA0.5431
OD+OSA0.5172

Comparison of phenotypic methods for the detection on methicillin resistance in S. aureus

Phenotypic methodsNo. of isolates showing mecA MRSA (n = 21)No of isolates showing non-mecA MSSA (n = 29)Statistical analysis (%)
No. of true positive isolatesNo. of false negative isolatesNo. of true negative isolatesNo. of false positive isolatesSNSPPPVNPVAUC
OSA1831415864854.5483.3564
MIC2101514100526010072
OD1831514865256.2583.366
CFD21018111006265.610078
eISSN:
1875-855X
Język:
Angielski
Częstotliwość wydawania:
6 razy w roku
Dziedziny czasopisma:
Medicine, Assistive Professions, Nursing, Basic Medical Science, other, Clinical Medicine