Rivista e Edizione

Volume 16 (2022): Edizione 4 (August 2022)

Volume 16 (2022): Edizione 3 (June 2022)

Volume 16 (2022): Edizione 2 (April 2022)

Volume 16 (2022): Edizione 1 (February 2022)

Volume 15 (2021): Edizione 6 (December 2021)

Volume 15 (2021): Edizione 5 (October 2021)

Volume 15 (2021): Edizione 4 (August 2021)

Volume 15 (2021): Edizione 3 (June 2021)

Volume 15 (2021): Edizione 2 (April 2021)

Volume 15 (2021): Edizione 1 (February 2021)

Volume 14 (2020): Edizione 6 (December 2020)

Volume 14 (2020): Edizione 5 (October 2020)

Volume 14 (2020): Edizione 4 (August 2020)

Volume 14 (2020): Edizione 3 (June 2020)

Volume 14 (2020): Edizione 2 (April 2020)

Volume 14 (2020): Edizione 1 (February 2020)

Volume 13 (2019): Edizione 6 (December 2019)

Volume 13 (2019): Edizione 5 (October 2019)

Volume 13 (2019): Edizione 4 (August 2019)

Volume 13 (2019): Edizione 3 (June 2019)

Volume 13 (2019): Edizione 2 (April 2019)

Volume 13 (2019): Edizione 1 (February 2019)

Volume 12 (2018): Edizione 6 (December 2018)

Volume 12 (2018): Edizione 5 (October 2018)

Volume 12 (2018): Edizione 4 (August 2018)

Volume 12 (2018): Edizione 3 (June 2018)

Volume 12 (2018): Edizione 2 (April 2018)

Volume 12 (2018): Edizione 1 (February 2018)

Volume 11 (2017): Edizione 6 (December 2017)

Volume 11 (2017): Edizione 5 (October 2017)

Volume 11 (2017): Edizione 4 (August 2017)

Volume 11 (2017): Edizione 3 (June 2017)

Volume 11 (2017): Edizione 2 (April 2017)

Volume 11 (2017): Edizione 1 (February 2017)

Volume 10 (2016): Edizione 6 (December 2016)

Volume 10 (2016): Edizione 5 (October 2016)

Volume 10 (2016): Edizione 4 (August 2016)

Volume 10 (2016): Edizione 3 (June 2016)

Volume 10 (2016): Edizione 2 (April 2016)

Volume 10 (2016): Edizione s1 (December 2016)

Volume 10 (2016): Edizione 1 (February 2016)

Volume 9 (2015): Edizione 6 (December 2015)

Volume 9 (2015): Edizione 5 (October 2015)

Volume 9 (2015): Edizione 4 (August 2015)

Volume 9 (2015): Edizione 3 (June 2015)

Volume 9 (2015): Edizione 2 (April 2015)

Volume 9 (2015): Edizione 1 (February 2015)

Volume 8 (2014): Edizione 6 (December 2014)

Volume 8 (2014): Edizione 5 (October 2014)

Volume 8 (2014): Edizione 4 (August 2014)

Volume 8 (2014): Edizione 3 (June 2014)

Volume 8 (2014): Edizione 2 (April 2014)

Volume 8 (2014): Edizione 1 (February 2014)

Volume 7 (2013): Edizione 6 (December 2013)

Volume 7 (2013): Edizione 5 (October 2013)

Volume 7 (2013): Edizione 4 (August 2013)

Volume 7 (2013): Edizione 3 (June 2013)

Volume 7 (2013): Edizione 2 (April 2013)

Volume 7 (2013): Edizione 1 (February 2013)

Volume 6 (2012): Edizione 6 (December 2012)

Volume 6 (2012): Edizione 5 (October 2012)

Volume 6 (2012): Edizione 4 (August 2012)

Volume 6 (2012): Edizione 3 (June 2012)

Volume 6 (2012): Edizione 2 (April 2012)

Volume 6 (2012): Edizione 1 (February 2012)

Volume 5 (2011): Edizione 6 (December 2011)

Volume 5 (2011): Edizione 5 (October 2011)

Volume 5 (2011): Edizione 4 (August 2011)

Volume 5 (2011): Edizione 3 (June 2011)

Volume 5 (2011): Edizione 2 (April 2011)

Volume 5 (2011): Edizione 1 (February 2011)

Volume 4 (2010): Edizione 6 (December 2010)

Volume 4 (2010): Edizione 5 (October 2010)

Volume 4 (2010): Edizione 4 (August 2010)

Volume 4 (2010): Edizione 3 (June 2010)

Volume 4 (2010): Edizione 2 (April 2010)

Volume 4 (2010): Edizione 1 (February 2010)

Dettagli della rivista
Formato
Rivista
eISSN
1875-855X
Pubblicato per la prima volta
01 Jun 2007
Periodo di pubblicazione
6 volte all'anno
Lingue
Inglese

Cerca

Volume 12 (2018): Edizione 5 (October 2018)

Dettagli della rivista
Formato
Rivista
eISSN
1875-855X
Pubblicato per la prima volta
01 Jun 2007
Periodo di pubblicazione
6 volte all'anno
Lingue
Inglese

Cerca

6 Articoli

Editorial

Open Access

Point-of-care test to detect hepatitis B virus DNA: Is it useful?

Pubblicato online: 01 Oct 2019
Pagine: 199 - 200

Astratto

Original article

Open Access

Development of a point-of-care test to detect hepatitis B virus DNA threshold relevant for treatment indication

Pubblicato online: 01 Oct 2019
Pagine: 201 - 209

Astratto

Abstract

Hepatitis B virus (HBV) has been the most prevalent blood-borne pathogen wherein utero transmission has still not been properly managed. Recent practice guidelines suggested that an antiviral drug should be administered to third-trimester pregnancies with significant viremia (>2 × 105 IU/mL).

To develop a novel turbidity-based loop-mediated isothermal amplification (LAMP) coupled with heat treatment DNA extraction method that is a rapid, cost-effective, and feasible viral load assessment and could be applied to antenatal screening.

Primers and reagents were designed, turbidity-based platform and heat treatment method were added, and evaluated for optimal efficiency. Assay sensitivity was tested from serially diluted standard HBV DNA. Assay specificity was tested with six standard viral DNAs. Clinical samples were analyzed and the results were compared with those of quantitative polymerase chain reaction (qPCR) diagnostic records.

The optimized condition was 60°C with no betaine, 1.4 mM deoxyribonucleotide triphosphates (dNTPs) and 6 mM of MgSO4 for 60 min. The assay accurately detected samples with standard HBV DNA at >2 × 105 IU/mL in both distilled water and spiked serum. Results can be interpreted within 31.48 ± 1.41 min in real-time turbidimeter. The amplification is exclusively specific to HBV, but not with the other six human-specific viruses. Moreover, the assay showed comparable performance within 95% confidence interval to the previously developed HBV LAMP toward clinical specimens.

This newly developed method was accurate, affordable, and flexible to further implementation to large-scale third-trimester pregnancy screening.

Parole chiave

  • diagnosis
  • hepatitis b
  • pregnancy
Open Access

Lactobacillus plantarum B7 attenuates Salmonella typhimurium infection in mice: preclinical study in vitro and in vivo

Pubblicato online: 01 Oct 2019
Pagine: 211 - 218

Astratto

Abstract

Salmonella typhimurium is a cause of gastroenteritis including diarrhea. Lactobacillus plantarum is a probiotic widely used to prevent and treat diarrhea.

To determine the protective effects of L. plantarum B7 on diarrhea in mice induced by S. typhimurium.

Inhibition of S. typhimurium growth by L. plantarum B7 was determined using an agar spot method. Mice were divided into 3 groups (n = 8 each): a control group, an S group administered 3 × 109 CFU/mL S. typhimurium, and an S + LP group administered 1 × 109 CFU/mL L. plantarum B7 and 3 × 109 CFU/mL S. typhimurium daily for 3 days. Counts of S. typhimurium and percentage of fecal moisture content (%FMC) were determined from stool samples. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and CXCL1 were determined.

L. plantarum B7 produced a clear zone on S. typhimurium. There were significantly less S. typhimurium in the feces from mice in the S+LP group than in the S group. Serum levels of TNF-α, IL-6, and CXCL1 in mice from the S group were significantly higher than levels in the S+LP and control groups. Feces from mice in the S group were soft and loose, whereas in the S+LP group they were hard and rod shaped. The %FMC in the S+LP group was significantly less than in the S group.

L. plantarum B7 can inhibit growth of S. typhimurium, decrease levels of proinflammatory cytokines, and attenuate symptoms of diarrhea induced in mice by S. typhimurium.

Parole chiave

  • bacteremia
  • diarrhea
  • gastroenteritis

Brief communication (original)

Open Access

Evidence of growth hormone effect on plasma leptin in diet-induced obesity and diet-resistant rats

Pubblicato online: 01 Oct 2019
Pagine: 219 - 228

Astratto

Abstract

Plasma leptin is regulated by several factors, including growth hormone (GH), which influences the pathophysiology of obesity.

To demonstrate the short-term effect of GH on plasma leptin levels in 3 conditions in vivo with the different amount of body fat mass.

Adult male Wistar rats were fed with standard chow or hypercaloric diet (HC). The HC rats were demonstrated as HC-feeding obese (HC-O) and HC-feeding resistant (HC-R) rats. Then, they were treated with GH or saline for 3 days. Basal plasma leptin levels were measured at 24 and 32 h. For meal-induced condition, all rats were fed for 2 hand plasma leptin was measured. Further 16-h fasting period, plasma leptin, insulin, and insulin sensitivity indexes were determined.

The short-term GH treatment decreased basal plasma leptin at 32 h after the first GH injection in HC-O rats. However, GH treatment had no effect on meal-induced plasma leptin in all rats. Furthermore, GH treatment attenuated fasting effect on plasma leptin in control and HC-R rats. The insulin resistance (IR) induced by the short-term GH treatment was demonstrated by higher fasting plasma insulin and the increased homeostasis model of IR in HC-R rats.

The study demonstrates the important role of greater fat mass in HC-O rats, which results in decreased basal plasma leptin after short-term GH treatment. For meal-induced condition, GH had no effect on plasma leptin in all rats. Interestingly, GH could attenuate fasting effect on plasma leptin in rats that have lower fat mass.

Parole chiave

  • adipose tissue
  • growth hormone
  • insulin resistance
  • leptin
  • obesity
Open Access

Sodium glycerophosphate and prescribed calcium concentrations in pediatric parenteral nutrition: a retrospective observational study and economic evaluation

Pubblicato online: 01 Oct 2019
Pagine: 229 - 235

Astratto

Abstract

The risk of precipitation limits calcium and phosphate concentrations that can be administered parenterally to pediatric patients. As an alternative to dipotassium phosphate, sodium glycerophosphate (NaGlyP) is claimed to reduce the risk of precipitation in solutions for parenteral administration.

To determine the calcium concentrations, NaGlyP, and dipotassium phosphate prescribed in pediatric parenteral nutrition orders and the cost–benefit of the organic phosphate.

We retrospectively collected cross-sectional data for parenteral nutrition orders from September 2014 to August 2015 for pediatric patients including neonates and children aged <18 years who were admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand. Calcium concentration, calcium concentration adjustments, and costs of phosphate used per bag were analyzed.

Of 2,192 parenteral nutrition orders, NaGlyP was used in 2,128 (97.1%) with calcium concentrations in the range of 0.84–139.91 mmol/L, which were significantly higher than calcium concentrations used with dipotassium phosphate (0.00–12.21 mmol/L, P < 0.001). There was no report of visible precipitation. Median costs of NaGlyP and dipotassium phosphate used per unit bag were not significantly different (35.88 and 41.25 Thai baht [THB] or 1.04 and 1.20 USD per bag, respectively, P>0.99; (1 USD equivalent to 34.241 THB U.S. Federal Reserve Bank G5.A annual average rate 2015).

Higher calcium concentrations could be achieved without increasing the direct cost per unit bag significantly as a result of using NaGlyP, an alternative to dipotassium phosphate as a source of phosphate for patients who require high amounts of calcium in parenteral nutrition.

Parole chiave

  • alpha-glycerophosphoric acid
  • glycerophosphates
  • calcium
  • chemical precipitation
  • parenteral nutrition
  • phosphates
Open Access

Outcomes of pancreaticoduodenectomy in patients with obstructive jaundice with and without preoperative biliary drainage: a retrospective observational study

Pubblicato online: 01 Oct 2019
Pagine: 237 - 241

Astratto

Abstract

Preoperative biliary drainage (PBD) in patients with obstructive jaundice from periampullary neoplasms may reduce the untoward effects of biliary obstruction and subsequent postoperative complications. However, PBD is associated with bile contamination and increases infectious complications after pancreaticoduodenectomy (PD).

To determine whether PBD is associated with more complications after PD.

Patients with obstructive jaundice from periampullary lesions who underwent PD from 2000 to 2015 at our institution were retrospectively enrolled. The cohort was divided into a group with PBD and a group without. PBD was performed using one of the following methods: endoprosthesis, percutaneous transhepatic biliary drainage, surgical biliary-enteric bypass, or T-tube choledochostomy. PDs were performed by the first author using uniform surgical techniques. Postoperative complications were recorded. Statistical analyses were conducted using an unpaired t, Fisher exact, or chi-squared tests as appropriate.

There were 26 with PBD and 28 patients without. Patients in the 2 groups were similar in age, presenting serum bilirubin level, operative time, operative blood transfusion, and hospital stay. The group with PBD had longer duration of jaundice, more patients presenting with cholangitis, and more patients with carcinoma of the ampulla of Vater. The overall complications were higher in patients in the group with PBD than in the group without.

PBD was associated with more complications overall after PD. However, PBD was necessary and lifesaving in certain clinical situations and improved the condition of patients before they underwent PD. Routine PBD in patients with obstructive jaundice without definite indications is not recommended.

Parole chiave

  • cholangitis
  • digestive system surgical procedures
  • drainage
  • jaundice
  • Pancreaticoduodenectomy
6 Articoli

Editorial

Open Access

Point-of-care test to detect hepatitis B virus DNA: Is it useful?

Pubblicato online: 01 Oct 2019
Pagine: 199 - 200

Astratto

Original article

Open Access

Development of a point-of-care test to detect hepatitis B virus DNA threshold relevant for treatment indication

Pubblicato online: 01 Oct 2019
Pagine: 201 - 209

Astratto

Abstract

Hepatitis B virus (HBV) has been the most prevalent blood-borne pathogen wherein utero transmission has still not been properly managed. Recent practice guidelines suggested that an antiviral drug should be administered to third-trimester pregnancies with significant viremia (>2 × 105 IU/mL).

To develop a novel turbidity-based loop-mediated isothermal amplification (LAMP) coupled with heat treatment DNA extraction method that is a rapid, cost-effective, and feasible viral load assessment and could be applied to antenatal screening.

Primers and reagents were designed, turbidity-based platform and heat treatment method were added, and evaluated for optimal efficiency. Assay sensitivity was tested from serially diluted standard HBV DNA. Assay specificity was tested with six standard viral DNAs. Clinical samples were analyzed and the results were compared with those of quantitative polymerase chain reaction (qPCR) diagnostic records.

The optimized condition was 60°C with no betaine, 1.4 mM deoxyribonucleotide triphosphates (dNTPs) and 6 mM of MgSO4 for 60 min. The assay accurately detected samples with standard HBV DNA at >2 × 105 IU/mL in both distilled water and spiked serum. Results can be interpreted within 31.48 ± 1.41 min in real-time turbidimeter. The amplification is exclusively specific to HBV, but not with the other six human-specific viruses. Moreover, the assay showed comparable performance within 95% confidence interval to the previously developed HBV LAMP toward clinical specimens.

This newly developed method was accurate, affordable, and flexible to further implementation to large-scale third-trimester pregnancy screening.

Parole chiave

  • diagnosis
  • hepatitis b
  • pregnancy
Open Access

Lactobacillus plantarum B7 attenuates Salmonella typhimurium infection in mice: preclinical study in vitro and in vivo

Pubblicato online: 01 Oct 2019
Pagine: 211 - 218

Astratto

Abstract

Salmonella typhimurium is a cause of gastroenteritis including diarrhea. Lactobacillus plantarum is a probiotic widely used to prevent and treat diarrhea.

To determine the protective effects of L. plantarum B7 on diarrhea in mice induced by S. typhimurium.

Inhibition of S. typhimurium growth by L. plantarum B7 was determined using an agar spot method. Mice were divided into 3 groups (n = 8 each): a control group, an S group administered 3 × 109 CFU/mL S. typhimurium, and an S + LP group administered 1 × 109 CFU/mL L. plantarum B7 and 3 × 109 CFU/mL S. typhimurium daily for 3 days. Counts of S. typhimurium and percentage of fecal moisture content (%FMC) were determined from stool samples. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and CXCL1 were determined.

L. plantarum B7 produced a clear zone on S. typhimurium. There were significantly less S. typhimurium in the feces from mice in the S+LP group than in the S group. Serum levels of TNF-α, IL-6, and CXCL1 in mice from the S group were significantly higher than levels in the S+LP and control groups. Feces from mice in the S group were soft and loose, whereas in the S+LP group they were hard and rod shaped. The %FMC in the S+LP group was significantly less than in the S group.

L. plantarum B7 can inhibit growth of S. typhimurium, decrease levels of proinflammatory cytokines, and attenuate symptoms of diarrhea induced in mice by S. typhimurium.

Parole chiave

  • bacteremia
  • diarrhea
  • gastroenteritis

Brief communication (original)

Open Access

Evidence of growth hormone effect on plasma leptin in diet-induced obesity and diet-resistant rats

Pubblicato online: 01 Oct 2019
Pagine: 219 - 228

Astratto

Abstract

Plasma leptin is regulated by several factors, including growth hormone (GH), which influences the pathophysiology of obesity.

To demonstrate the short-term effect of GH on plasma leptin levels in 3 conditions in vivo with the different amount of body fat mass.

Adult male Wistar rats were fed with standard chow or hypercaloric diet (HC). The HC rats were demonstrated as HC-feeding obese (HC-O) and HC-feeding resistant (HC-R) rats. Then, they were treated with GH or saline for 3 days. Basal plasma leptin levels were measured at 24 and 32 h. For meal-induced condition, all rats were fed for 2 hand plasma leptin was measured. Further 16-h fasting period, plasma leptin, insulin, and insulin sensitivity indexes were determined.

The short-term GH treatment decreased basal plasma leptin at 32 h after the first GH injection in HC-O rats. However, GH treatment had no effect on meal-induced plasma leptin in all rats. Furthermore, GH treatment attenuated fasting effect on plasma leptin in control and HC-R rats. The insulin resistance (IR) induced by the short-term GH treatment was demonstrated by higher fasting plasma insulin and the increased homeostasis model of IR in HC-R rats.

The study demonstrates the important role of greater fat mass in HC-O rats, which results in decreased basal plasma leptin after short-term GH treatment. For meal-induced condition, GH had no effect on plasma leptin in all rats. Interestingly, GH could attenuate fasting effect on plasma leptin in rats that have lower fat mass.

Parole chiave

  • adipose tissue
  • growth hormone
  • insulin resistance
  • leptin
  • obesity
Open Access

Sodium glycerophosphate and prescribed calcium concentrations in pediatric parenteral nutrition: a retrospective observational study and economic evaluation

Pubblicato online: 01 Oct 2019
Pagine: 229 - 235

Astratto

Abstract

The risk of precipitation limits calcium and phosphate concentrations that can be administered parenterally to pediatric patients. As an alternative to dipotassium phosphate, sodium glycerophosphate (NaGlyP) is claimed to reduce the risk of precipitation in solutions for parenteral administration.

To determine the calcium concentrations, NaGlyP, and dipotassium phosphate prescribed in pediatric parenteral nutrition orders and the cost–benefit of the organic phosphate.

We retrospectively collected cross-sectional data for parenteral nutrition orders from September 2014 to August 2015 for pediatric patients including neonates and children aged <18 years who were admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand. Calcium concentration, calcium concentration adjustments, and costs of phosphate used per bag were analyzed.

Of 2,192 parenteral nutrition orders, NaGlyP was used in 2,128 (97.1%) with calcium concentrations in the range of 0.84–139.91 mmol/L, which were significantly higher than calcium concentrations used with dipotassium phosphate (0.00–12.21 mmol/L, P < 0.001). There was no report of visible precipitation. Median costs of NaGlyP and dipotassium phosphate used per unit bag were not significantly different (35.88 and 41.25 Thai baht [THB] or 1.04 and 1.20 USD per bag, respectively, P>0.99; (1 USD equivalent to 34.241 THB U.S. Federal Reserve Bank G5.A annual average rate 2015).

Higher calcium concentrations could be achieved without increasing the direct cost per unit bag significantly as a result of using NaGlyP, an alternative to dipotassium phosphate as a source of phosphate for patients who require high amounts of calcium in parenteral nutrition.

Parole chiave

  • alpha-glycerophosphoric acid
  • glycerophosphates
  • calcium
  • chemical precipitation
  • parenteral nutrition
  • phosphates
Open Access

Outcomes of pancreaticoduodenectomy in patients with obstructive jaundice with and without preoperative biliary drainage: a retrospective observational study

Pubblicato online: 01 Oct 2019
Pagine: 237 - 241

Astratto

Abstract

Preoperative biliary drainage (PBD) in patients with obstructive jaundice from periampullary neoplasms may reduce the untoward effects of biliary obstruction and subsequent postoperative complications. However, PBD is associated with bile contamination and increases infectious complications after pancreaticoduodenectomy (PD).

To determine whether PBD is associated with more complications after PD.

Patients with obstructive jaundice from periampullary lesions who underwent PD from 2000 to 2015 at our institution were retrospectively enrolled. The cohort was divided into a group with PBD and a group without. PBD was performed using one of the following methods: endoprosthesis, percutaneous transhepatic biliary drainage, surgical biliary-enteric bypass, or T-tube choledochostomy. PDs were performed by the first author using uniform surgical techniques. Postoperative complications were recorded. Statistical analyses were conducted using an unpaired t, Fisher exact, or chi-squared tests as appropriate.

There were 26 with PBD and 28 patients without. Patients in the 2 groups were similar in age, presenting serum bilirubin level, operative time, operative blood transfusion, and hospital stay. The group with PBD had longer duration of jaundice, more patients presenting with cholangitis, and more patients with carcinoma of the ampulla of Vater. The overall complications were higher in patients in the group with PBD than in the group without.

PBD was associated with more complications overall after PD. However, PBD was necessary and lifesaving in certain clinical situations and improved the condition of patients before they underwent PD. Routine PBD in patients with obstructive jaundice without definite indications is not recommended.

Parole chiave

  • cholangitis
  • digestive system surgical procedures
  • drainage
  • jaundice
  • Pancreaticoduodenectomy

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