Purification and Biochemical Characterization of α-Amylase from Newly Isolated Bacillus Cereus Strain and its Application as an Additive in Breadmaking

Arcus VL, van der Kamp MW, Pudney CR, Mulholland AJ. Enzyme evolution and the temperature dependence of enzyme catalysis. Curr Opin Struct Biol. 2020 Dec;65:96–101. https://doi.org/10.1016/j.sbi.2020.06.001 Arcus VL van der Kamp MW Pudney CR Mulholland AJ. Enzyme evolution and the temperature dependence of enzyme catalysis . Curr Opin Struct Biol . 2020 Dec ; 65 : 96 – 101 . https://doi.org/10.1016/j.sbi.2020.06.001 Search in Google Scholar

Atudorei D, Atudorei O, Codină GG. Dough rheological properties, microstructure and bread quality of wheat-germinated bean composite flour. Foods. 2021 Jul;10(7)1542. https://doi.org/10.3390/foods10071542 Atudorei D Atudorei O Codină GG. Dough rheological properties, microstructure and bread quality of wheat-germinated bean composite flour . Foods . 2021 Jul ; 10 ( 7 ): 1542 . https://doi.org/10.3390/foods10071542 Search in Google Scholar

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem. 1976 May;72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding . Anal Biochem . 1976 May ; 72 : 248 – 254 . https://doi.org/10.1016/0003-2697(76)90527-3 Search in Google Scholar

Carroll LM, Cheng RA, Wiedmann M, Kovac J. Keeping up with the Bacillus cereus group: Taxonomy through the genomics era and beyond. Crit Rev Food Sci Nutr. 2022;62(28):7677–7702. https://doi.org/10.1080/10408398.2021.1916735 Carroll LM Cheng RA Wiedmann M Kovac J. Keeping up with the Bacillus cereus group: Taxonomy through the genomics era and beyond . Crit Rev Food Sci Nutr . 2022 ; 62 ( 28 ): 7677 – 7702 . https://doi.org/10.1080/10408398.2021.1916735 Search in Google Scholar

da Costa-Latgé SG, Bates P, Dillon R, Genta FA.Characterization of glycoside hydrolase families 13 and 31 reveals expansion and diversification of α-amylase genes in the phlebotomine Lutzomyia longipalpis and modulation of sandfly glycosidase activities by Leish-mania infection. Front Physiol. 2021 Apr;12:635633. https://doi.org/10.3389/fphys.2021.635633 da Costa-Latgé SG Bates P Dillon R Genta FA. Characterization of glycoside hydrolase families 13 and 31 reveals expansion and diversification of α-amylase genes in the phlebotomine Lutzomyia longipalpis and modulation of sandfly glycosidase activities by Leish-mania infection . Front Physiol . 2021 Apr ; 12 : 635633 . https://doi.org/10.3389/fphys.2021.635633 Search in Google Scholar

Dahiya S, Bajaj BK, Kumar A, Tiwari SK, Singh B. A review on biotechnological potential of multifarious enzymes in bread making. Proc Biochem. 2020 Dec;99:290–306. https://doi.org/10.1016/j.procbio.2020.09.002 Dahiya S Bajaj BK Kumar A Tiwari SK Singh B. A review on biotechnological potential of multifarious enzymes in bread making . Proc Biochem . 2020 Dec ; 99 : 290 – 306 . https://doi.org/10.1016/j.procbio.2020.09.002 Search in Google Scholar

Elyasi Far B, Ahmadi Y, Yari Khosroshahi A, Dilmaghani A. Microbial alpha-amylase production: Progress, challenges and perspectives. Adv Pharm Bull. 2020 Jul;10(3):350–358. https://doi.org/10.34172/apb.2020.043 Elyasi Far B Ahmadi Y Yari Khosroshahi A Dilmaghani A. Microbial alpha-amylase production: Progress, challenges and perspectives . Adv Pharm Bull . 2020 Jul ; 10 ( 3 ): 350 – 358 . https://doi.org/10.34172/apb.2020.043 Search in Google Scholar

Farooq MA, Ali S, Hassan A, Tahir HM, Mumtaz S, Mumtaz S. Biosynthesis and industrial applications of α-amylase: A review. Arch Microbiol. 2021 May;203(4):1281–1292. https://doi.org/10.1007/s00203-020-02128-y Farooq MA Ali S Hassan A Tahir HM Mumtaz S Mumtaz S. Biosynthesis and industrial applications of α-amylase: A review . Arch Microbiol . 2021 May ; 203 ( 4 ): 1281 – 1292 . https://doi.org/10.1007/s00203-020-02128-y Search in Google Scholar

Gazali FM, Suwastika IN. Thermostable α-amylase activity from thermophilic bacteria isolated from Bora Hot Spring, Central Sulawesi. In J Phys Conf Ser. 2018 Mar;979:012001. https://doi.org/10.1088/1742-6596/979/1/012001 Gazali FM Suwastika IN. Thermostable α-amylase activity from thermophilic bacteria isolated from Bora Hot Spring, Central Sulawesi . In J Phys Conf Ser . 2018 Mar ; 979 : 012001 . https://doi.org/10.1088/1742-6596/979/1/012001 Search in Google Scholar

Giri SS. Application of microbial biosurfactants in the pharmaceutical industry. In: Inamuddin, Ahamed MI, Prasad R, editors. Microbial biosurfactants. Environmental and microbial biotechnology. Singapore: Springer; 2021. https://doi.org/10.1007/978-981-15-6607-3_12 Giri SS. Application of microbial biosurfactants in the pharmaceutical industry . In: Inamuddin Ahamed MI Prasad R , editors. Microbial biosurfactants. Environmental and microbial biotechnology . Singapore : Springer ; 2021 . https://doi.org/10.1007/978-981-15-6607-3_12 Search in Google Scholar

Goesaert H, Slade L, Levine H, Delcour JA. Amylases and bread firming-An integrated view. J Cereal Sci. 2009 Nov;50(3):345–352. https://doi.org/10.1016/j.jcs.2009.04.010 Goesaert H Slade L Levine H Delcour JA. Amylases and bread firming–An integrated view . J Cereal Sci . 2009 Nov ; 50 ( 3 ): 345 – 352 . https://doi.org/10.1016/j.jcs.2009.04.010 Search in Google Scholar

Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B. Microbial α-amylases: A biotechnological perspective. Proc Bio-chem. 2003 Jun;38(11):1599–1616. https://doi.org/10.1016/s0032-9592(03)00053-0 Gupta R Gigras P Mohapatra H Goswami VK Chauhan B. Microbial α-amylases: A biotechnological perspective . Proc Bio-chem . 2003 Jun ; 38 ( 11 ): 1599 – 1616 . https://doi.org/10.1016/s0032-9592(03)00053-0 Search in Google Scholar

Immonen M, Maina NH, Coda R, Katina K. 5 Upcycling of surplus bread using tailored biotransformation. In: Hakalehto E, editor. Microbiology of food quality: Challenges in food production and distribution during and after the pandemics. Berlin (Germany): de Gruyter; 2021. p. 99–106. https://doi.org/10.1515/9783110724967-006 Immonen M Maina NH Coda R Katina K. 5 Upcycling of surplus bread using tailored biotransformation . In: Hakalehto E , editor. Microbiology of food quality: Challenges in food production and distribution during and after the pandemics . Berlin (Germany) : de Gruyter ; 2021 . p. 99 – 106 . https://doi.org/10.1515/9783110724967-006 Search in Google Scholar

Jujjavarapu SE, Dhagat S. Evolutionary trends in industrial production of α-amylase. Recent Pat Biotechnol. 2019;13(1):4–18. https://doi.org/10.2174/2211550107666180816093436 Jujjavarapu SE Dhagat S. Evolutionary trends in industrial production of α-amylase . Recent Pat Biotechnol . 2019 ; 13 ( 1 ): 4 – 18 . https://doi.org/10.2174/2211550107666180816093436 Search in Google Scholar

Kohli I, Joshi NC, Varma A. Production, purification and applications of raw starch degrading and calcium-independent α-amylase from soil rich in extremophile. Int J Biol Macromol. 2020 Nov;162:873–881. https://doi.org/10.1016/j.ijbiomac.2020.06.160 Kohli I Joshi NC Varma A. Production, purification and applications of raw starch degrading and calcium-independent α-amylase from soil rich in extremophile . Int J Biol Macromol . 2020 Nov ; 162 : 873 – 881 . https://doi.org/10.1016/j.ijbiomac.2020.06.160 Search in Google Scholar

Kumari S, Sarkar PK. Bacillus cereus hazard and control in industrial dairy processing environment. Food cont. 2016 Nov; 69:20–29. https://doi.org/10.1016/j.foodcont.2016.04.012 Kumari S Sarkar PK. Bacillus cereus hazard and control in industrial dairy processing environment . Food cont . 2016 Nov ; 69 : 20 – 29 . https://doi.org/10.1016/j.foodcont.2016.04.012 Search in Google Scholar

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug;227(5259):680–685. https://doi.org/10.1038/227680a0 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature . 1970 Aug ; 227 ( 5259 ): 680 – 685 . https://doi.org/10.1038/227680a0 Search in Google Scholar

Mahmoud E, Ramadan M, Ismail MM, Fadel M, Abass M. Production of flavors from agro waste of Ocimumbasilicum L. by different microorganisms using solid state fermentation. Egyp. J Chem. 2022 Jul;65(7):259–273. https://doi.org/10.21608/ejchem.2021.103622.4794 Mahmoud E Ramadan M Ismail MM Fadel M Abass M. Production of flavors from agro waste of Ocimumbasilicum L . by different microorganisms using solid state fermentation. Egyp. J Chem . 2022 Jul ; 65 ( 7 ): 259 – 273 . https://doi.org/10.21608/ejchem.2021.103622.4794 Search in Google Scholar

Ndochinwa OG, Wang QY, Amadi OC, Nwagu TN, Nnamchi CI, Moneke AN. Pullulanase functions in starch hydrolysis and the improvement of enzyme activity using active hydrogen bond network as method with potential industrial application of the enzyme. J Global Biosci. 2021;10(5):8685–8705. Ndochinwa OG Wang QY Amadi OC Nwagu TN Nnamchi CI Moneke AN. Pullulanase functions in starch hydrolysis and the improvement of enzyme activity using active hydrogen bond network as method with potential industrial application of the enzyme . J Global Biosci . 2021 ; 10 ( 5 ): 8685 – 8705 . Search in Google Scholar

Ottone C, Romero O, Aburto C, Illanes A, Wilson L. Biocatalysis in the winemaking industry: Challenges and opportunities for immobilized enzymes. Compr Rev Food Sci Food Saf. 2020 Mar;19(2):595–621. https://doi.org/10.1111/1541-4337.12538 Ottone C Romero O Aburto C Illanes A Wilson L. Biocatalysis in the winemaking industry: Challenges and opportunities for immobilized enzymes . Compr Rev Food Sci Food Saf . 2020 Mar ; 19 ( 2 ): 595 – 621 . https://doi.org/10.1111/1541-4337.12538 Search in Google Scholar

Parmar FA, Patel JN, Upasani VN. Screening of microorganisms for production of therapeutic enzymes. Eur J Biotechnol Biosci. 2021;9(3):82–88. Parmar FA Patel JN Upasani VN. Screening of microorganisms for production of therapeutic enzymes . Eur J Biotechnol Biosci . 2021 ; 9 ( 3 ): 82 – 88 . Search in Google Scholar

Petrović D, Risso VA, Kamerlin SCL, Sanchez-Ruiz JM. Conformational dynamics and enzyme evolution. J R Soc Interface. 2018 Jul;15(144):20180330. https://doi.org/10.1098/rsif.2018.0330 Petrović D Risso VA Kamerlin SCL Sanchez-Ruiz JM. Conformational dynamics and enzyme evolution . J R Soc Interface . 2018 Jul ; 15 ( 144 ): 20180330 . https://doi.org/10.1098/rsif.2018.0330 Search in Google Scholar

Prakash O, Jaiswal N. α-Amylase: An ideal representative of thermostable enzymes. Appl Biochem Biotechnol. 2010 Apr; 160(8): 2401–2414. https://doi.org/10.1007/s12010-009-8735-4 Prakash O Jaiswal N. α-Amylase: An ideal representative of thermostable enzymes . Appl Biochem Biotechnol . 2010 Apr ; 160 ( 8 ): 2401 – 2414 . https://doi.org/10.1007/s12010-009-8735-4 Search in Google Scholar

Rasooli I, Astaneh SD, Borna H, Barchini KA. A thermostable α-amylase producing natural variant of Bacillus spp. isolated from soil in Iran. Am. J Agric Biol Sci. 2008 Sep;3(3):591–596. https://doi.org/10.3844/ajabssp.2008.591.596 Rasooli I Astaneh SD Borna H Barchini KA. A thermostable α-amylase producing natural variant of Bacillus spp . isolated from soil in Iran. Am. J Agric Biol Sci . 2008 Sep ; 3 ( 3 ): 591 – 596 . https://doi.org/10.3844/ajabssp.2008.591.596 Search in Google Scholar

Sadeghian Motahar SF, Salami M, Ariaeenejad S, Emam-Djomeh Z, Sheykh Abdollahzadeh Mamaghani A, Kavousi K, Moghadam M, Hosseini Salekdeh G. Synergistic effect of metagenome-derived starch-degrading enzymes on quality of functional bread with antioxidant activity. Starch-Stärke. 2022 Jan;74(1–2):2100098. https://doi.org/10.1002/star.202100098 Sadeghian Motahar SF Salami M Ariaeenejad S Emam-Djomeh Z Sheykh Abdollahzadeh Mamaghani A Kavousi K Moghadam M Hosseini Salekdeh G. Synergistic effect of metagenome-derived starch-degrading enzymes on quality of functional bread with antioxidant activity . Starch-Stärke . 2022 Jan ; 74 ( 1–2 ): 2100098 . https://doi.org/10.1002/star.202100098 Search in Google Scholar

Salem K, Elgharbi F, Ben Hlima H, Perduca M, Sayari A, Hmida-Sayari A. Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca²⁺ independent Bacillus subtilis α-amylase. Biotechnol Prog. 2020 Jul;36(4):e2964. https://doi.org/10.1002/btpr.2964 Salem K Elgharbi F Ben Hlima H Perduca M Sayari A Hmida-Sayari A. Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca²⁺ independent Bacillus subtilis α-amylase . Biotechnol Prog . 2020 Jul ; 36 ( 4 ): e2964 . https://doi.org/10.1002/btpr.2964 Search in Google Scholar

Shirling ET, Gottlieb D. Methods for characterization of Strepto-myces species. Int J Syst Bacteriol. 1966 Jul;16(3):313–340. https://doi.org/10.1099/00207713-16-3-313 Shirling ET Gottlieb D. Methods for characterization of Strepto-myces species . Int J Syst Bacteriol . 1966 Jul ; 16 ( 3 ): 313 – 340 . https://doi.org/10.1099/00207713-16-3-313 Search in Google Scholar

Shirodkar PV, Muraleedharan UD, Damare S, Raghukumar S. A mesohaline thraustochytrid produces extremely halophilic alpha-amylases. Mar Biotechnol. 2020 Jun;22(3):403–410. https://doi.org/10.1007/s10126-020-09960-9 Shirodkar PV Muraleedharan UD Damare S Raghukumar S. A mesohaline thraustochytrid produces extremely halophilic alphaamylases . Mar Biotechnol . 2020 Jun ; 22 ( 3 ): 403 – 410 . https://doi.org/10.1007/s10126-020-09960-9 Search in Google Scholar

Silaban S, Marika DB, Simorangkir M. Isolation and characterization of amylase-producing amylolytic bacteria from rice soil samples. J Phys Conf Ser. 2020;1485(1):012006 https://doi.org/10.1088/1742-6596/1485/1/012006 Silaban S Marika DB Simorangkir M. Isolation and characterization of amylase-producing amylolytic bacteria from rice soil samples . J Phys Conf Ser . 2020 ; 1485 ( 1 ): 012006 https://doi.org/10.1088/1742-6596/1485/1/012006 Search in Google Scholar

Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov;22(22):4673–4680. https://doi.org/10.1093/nar/22.22.4673 Thompson JD Higgins DG Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice . Nucleic Acids Res . 1994 Nov ; 22 ( 22 ): 4673 – 4680 . https://doi.org/10.1093/nar/22.22.4673 Search in Google Scholar

Timilsina PM, Pandey GR, Shrestha AS, Ojha M, Baral G, Karki TB. Purification and characterization of a noble thermostable alpha-amylase from Anoxybacillus tengchongensis RA1-2-1 isolated from geothermal spring of Nepal. J Food Sci Technol Nepal. 2020 Dec; 12(12):49–58. https://doi.org/10.3126/jfstn.v12i12.31038 Timilsina PM Pandey GR Shrestha AS Ojha M Baral G Karki TB. Purification and characterization of a noble thermostable alpha-amylase from Anoxybacillus tengchongensis RA1-2-1 isolated from geothermal spring of Nepal . J Food Sci Technol Nepal . 2020 Dec ; 12 ( 12 ): 49 – 58 . https://doi.org/10.3126/jfstn.v12i12.31038 Search in Google Scholar

Williams S T, Goodfellow M, Alderson G. Genus Streptomyces Waksman and Henrici 1943, 339^AL. In: Williams ST, Sharpe ME, Holt JG, editors. Bergey’s manual of systematic bacteriology. Baltimore (USA): Williams &, Wilkins; 1989. p. 2452–2492. Williams S T Goodfellow M Alderson G. Genus Streptomyces Waksman and Henrici 1943, 339^AL . In: Williams ST Sharpe ME Holt JG , editors. Bergey’s manual of systematic bacteriology . Baltimore (USA) : Williams &, Wilkins ; 1989 . p. 2452 – 2492 . Search in Google Scholar