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Detection by Whole-Genome Sequencing of a Novel Metallo-β-Lactamase Produced by Wautersiella falsenii Causing Urinary Tract Infection in Tunisia

Raouaa Maaroufi

Raouaa Maaroufi

  • Aix-Marseille Université MEPHI, AP-HM, IRD, IHU Méditerranée InfectionMarseille, France
  • Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis El-ManarTunis, Tunisie
  • Laboratoire de Recherche Sciences et Technologies de l’Environnement, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Hammam-Lif Université de CarthageTunisie
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Maaroufi, Raouaa
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Olfa Dziri

Olfa Dziri

  • Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis El-ManarTunis, Tunisie
  • Laboratoire de Recherche Sciences et Technologies de l’Environnement, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Hammam-Lif Université de CarthageTunisie
  • Unité de Service en Commun pour la Recherche « Plateforme Génomique », Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Hammam-Lif, Université de CarthageTunisie
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Dziri, Olfa
, 
Linda Hadjadj

Linda Hadjadj

Aix-Marseille Université MEPHI, AP-HM, IRD, IHU Méditerranée InfectionMarseille, France
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Hadjadj, Linda
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Seydina M. Diene

Seydina M. Diene

Aix-Marseille Université MEPHI, AP-HM, IRD, IHU Méditerranée InfectionMarseille, France
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Diene, Seydina M.
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Jean-Marc Rolain

Jean-Marc Rolain

Aix-Marseille Université MEPHI, AP-HM, IRD, IHU Méditerranée InfectionMarseille, France
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Rolain, Jean-Marc
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Chedly Chouchani

Chedly Chouchani

  • Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis El-ManarTunis, Tunisie
  • Laboratoire de Recherche Sciences et Technologies de l’Environnement, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Hammam-Lif Université de CarthageTunisie
  • Unité de Service en Commun pour la Recherche « Plateforme Génomique », Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Hammam-Lif, Université de CarthageTunisie
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Chouchani, Chedly
  
27 feb 2022
Detection by Whole-Genome Sequencing of a Novel Metallo-β-Lactamase Produced by Wautersiella falsenii Causing Urinary Tract Infection in Tunisia's Cover Image
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Categoria dell'articolo: Original Paper
Pubblicato online: 27 feb 2022
Pagine: 73 - 81
Ricevuto: 20 ago 2021
Accettato: 22 nov 2021
DOI: https://doi.org/10.33073/pjm-2022-010
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© 2022 Raouaa Maaroufi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

Phylogenetic tree based on the protein sequences of; serine β-lactamase (Class A and Class C) and metallo-β-lactamase (Class B).
Phylogenetic tree based on the protein sequences of; serine β-lactamase (Class A and Class C) and metallo-β-lactamase (Class B).

Fig. 2

Protein alignment among the metallo-β-lactamase type B genes: Our sequence was represented with red color. The conserved motif of the MBL “HxHxDH” presented in their active site, and residues of bacterial metallo-β-lactamase enzymes were represented with a yellow color.
Protein alignment among the metallo-β-lactamase type B genes: Our sequence was represented with red color. The conserved motif of the MBL “HxHxDH” presented in their active site, and residues of bacterial metallo-β-lactamase enzymes were represented with a yellow color.

Comparison between all reported Wautersiella falsenii bacterial infections and that of the present study_

Reference Number of isolates Years of isolation Country Gender and age of infected patients Patient suffered from Clinical specimens Phenotypic resistance pattern Detected genes Detected plasmids
Kämpfer et al. 2006 26 1980–2004 Belgium – – blood, wounds, pus, respiratory tract, ear discharge, oral cavity, vaginal swab, pleural fluid, and other from an unknown origin – – –
Van der Velden et al. 2012 1 2012 Netherlands one-year-old girl pyelonephritis urine nitrofurantoin, amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftriaxone, ceftazidime, meropenem, tobramycin, and colistin – –
Traglia et al. 2015 1 2013 Argentina 18-year-old woman acute otitis media cervical neck abscess ampicillin, ampicillin-sulbactam, cephalothin, meropenem, colistin 29 genes coding for efflux pumps, and tripartite multidrug resistance systems, class a β-lactamase, metallo-β-lactamase (ebr-2), three class c β-lactamases, resistance to streptogramin trimethoprim, bacitracin, and macrolide none
Giordano et al. 2016 1 2016 Italy 32-year-old man lymphoblastic leukemia the respiratory tract amikacin, amikacin-clavulanic acid, ampicillin-sulbactam, cefotaxime, ceftazidime, doripenem, gentamicin, imipenem, piperacillin-tazobactam – –
Zaman et al. 2017 1 2017 India five-year-old boy bladder cancer urine ampicillin, ampicillin-sulbactam, and colistin. – –
This study 1 2016 Tunisia 35-year-old woman UTI urine amoxicillin, amoxicillin-clavulanic acid, ticarcillin, ticarcillin-clavulanic acid, amikacin, tobramycin, streptomycin, spectinomycin, ciprofloxacin, pefloxacin, nalidixic acid, furans, nitrofurantoin blaEBR-like, TetX and aadS none
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Microbiologia e virologia
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