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Campylobacter fetus Induced Proinflammatory Response in Bovine Endometrial Epithelial Cells

LIZETH GUADALUPE CAMPOS-MÚZQUIZ

LIZETH GUADALUPE CAMPOS-MÚZQUIZ

Doctorado en Ciencias Agropecuarias, Universidad Autónoma Metropolitana XochimilcoJohor, México
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CAMPOS-MÚZQUIZ, LIZETH GUADALUPE
, 
ESTELA TERESITA MÉNDEZ-OLVERA

ESTELA TERESITA MÉNDEZ-OLVERA

Laboratorio de Biología Molecular, Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana XochimilcoJohor, México
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MÉNDEZ-OLVERA, ESTELA TERESITA
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MONIKA PALACIOS MARTÍNEZ

MONIKA PALACIOS MARTÍNEZ

Laboratorio de Biología Molecular, Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana XochimilcoJohor, México
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MARTÍNEZ, MONIKA PALACIOS
 e 
DANIEL MARTÍNEZ-GÓMEZ

DANIEL MARTÍNEZ-GÓMEZ

Laboratorio de Microbiología Agropecuaria, Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana XochimilcoJohor, México
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MARTÍNEZ-GÓMEZ, DANIEL
  
19 mar 2021
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Categoria dell'articolo: original-paper
Pubblicato online: 19 mar 2021
Pagine: 99 - 106
Ricevuto: 01 nov 2020
Accettato: 26 gen 2021
DOI: https://doi.org/10.33073/pjm-2021-009
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© 2021 Lizeth Guadalupe Campos-Múzquiz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Salmonella Typhimurium and Campylobacter fetus subsp. fetus invasion assays in endometrial cells. A gentamicin protection assay was performed to demonstrate that C. fetus subsp. fetus invades bovine endometrial epithelial cells but does not survive.
Salmonella Typhimurium and Campylobacter fetus subsp. fetus invasion assays in endometrial cells. A gentamicin protection assay was performed to demonstrate that C. fetus subsp. fetus invades bovine endometrial epithelial cells but does not survive.

Fig. 2.

Intracellular survival expression analysis of Campylobacter fetus subsp. fetus and Salmonella Typhimurium in bovine endometrial epithelial cells. An invasion assay was performed, and RNA was extracted from cells. The cDNA was synthesized using random primers, and a quantitative PCR was performed.
Intracellular survival expression analysis of Campylobacter fetus subsp. fetus and Salmonella Typhimurium in bovine endometrial epithelial cells. An invasion assay was performed, and RNA was extracted from cells. The cDNA was synthesized using random primers, and a quantitative PCR was performed.

Fig. 3.

Interleukin expression in bovine endometrial epithelial cells challenged with Campylobacter fetus subsp. fetus or Salmonella Typhimurium. Expression was analyzed with a 2–ΔΔCT and compared to control cells (no infected). A student t-test was performed to ΔCT (CT gene of interest – geometric media CT housekeeping genes) compared against non-treated cells; *p < 0.05.
Interleukin expression in bovine endometrial epithelial cells challenged with Campylobacter fetus subsp. fetus or Salmonella Typhimurium. Expression was analyzed with a 2–ΔΔCT and compared to control cells (no infected). A student t-test was performed to ΔCT (CT gene of interest – geometric media CT housekeeping genes) compared against non-treated cells; *p < 0.05.

Fig. 4.

Interleukin expression in bovine endometrial epithelial cells treated with cytochalasin D and challenged with Campylobacter fetus subsp. fetus. Expression was analyzed with a 2–ΔΔCT and compared to control cells (non-cytochalasin D-treated cells). A student t-test was performed to the ΔCT (CT gene of interest – geometric media CT housekeeping genes) comparing against non-cytochalasin D-treated cells; *p < 0.05.
Interleukin expression in bovine endometrial epithelial cells treated with cytochalasin D and challenged with Campylobacter fetus subsp. fetus. Expression was analyzed with a 2–ΔΔCT and compared to control cells (non-cytochalasin D-treated cells). A student t-test was performed to the ΔCT (CT gene of interest – geometric media CT housekeeping genes) comparing against non-cytochalasin D-treated cells; *p < 0.05.
Lingua:
Inglese
Frequenza di pubblicazione:
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Argomenti della rivista:
Scienze biologiche, Microbiologia e virologia
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