<abstract xmlns="http://www.w3.org/1999/xhtml">

Despite advances in diagnosis and treatment, tuberculosis (TB) continues to be one of the essential health problems throughout the world. Turkey is considered to be endemic for TB. In this study, we analyzed the distribution of <italic>Mycobacterium</italic> species, compare the diagnostic methods, and susceptibilities to anti-tuberculosis drugs of TB isolates. The aim was to document the current status and to provide a frame of reference for future studies. In this study, 278 <italic>Mycobacterium</italic> species isolated from 7,480 patients between September 2015 and June 2019 were included. Löwenstein-Jensen medium (LJ) and MGIT 960 were used for the isolation of strains. Susceptibility to 1st-line anti-tuberculosis drugs was determined. Positivity rates in clinical samples were as follows: 1.4% for direct microscopic acid-fast bacilli (AFB) detection, 3.4% for growth on the LJ, and 3.7% for growth on MGIT-960. Two hundred thirty-three isolates were identified as <italic>Mycobacterium tuberculosis</italic> complex (MTBC) and 45 were non-tuberculous mycobacteria (NTMs). Eleven of the NTMs (24.4%) were <italic>Mycobacterium fortuitum</italic> group isolates, and eight NTMs (17.7%) were <italic>Mycobacterium abscessus</italic> complex isolates. A number of patients diagnosed with tuberculosis peaked twice between the ages of 20–31 and 60–71. A hundred and eighty-two MTBC isolates (78.1%) were susceptible to all 1st-line anti-tuberculosis drugs, while 51 isolates (21.9%) were resistant to at least one drug tested. The multidrug-resistant tuberculosis rate was 13.7% among resistant strains and 3% in all strains. The liquid cultures were better for detection of both MTBC and NTMs isolates. The data demonstrate that MTBC continues to be challenge for this country and indicates the need for continued surveillance and full-spectrum services of mycobacteriology laboratory and infectious diseases.
</abstract>

Despite advances in diagnosis and treatment, tuberculosis (TB) continues to be one of the essential health problems throughout the world. Turkey is considered to be endemic for TB. In this study, we analyzed the distribution of Mycobacterium species, compare the diagnostic methods, and susceptibilities to anti-tuberculosis drugs of TB isolates. The aim was to document the current status and to provide a frame of reference for future studies. In this study, 278 Mycobacterium species isolated from 7,480 patients between September 2015 and June 2019 were included. Löwenstein-Jensen medium (LJ) and MGIT 960 were used for the isolation of strains. Susceptibility to 1st-line anti-tuberculosis drugs was determined. Positivity rates in clinical samples were as follows: 1.4% for direct microscopic acid-fast bacilli (AFB) detection, 3.4% for growth on the LJ, and 3.7% for growth on MGIT-960. Two hundred thirty-three isolates were identified as Mycobacterium tuberculosis complex (MTBC) and 45 were non-tuberculous mycobacteria (NTMs). Eleven of the NTMs (24.4%) were Mycobacterium fortuitum group isolates, and eight NTMs (17.7%) were Mycobacterium abscessus complex isolates. A number of patients diagnosed with tuberculosis peaked twice between the ages of 20–31 and 60–71. A hundred and eighty-two MTBC isolates (78.1%) were susceptible to all 1st-line anti-tuberculosis drugs, while 51 isolates (21.9%) were resistant to at least one drug tested. The multidrug-resistant tuberculosis rate was 13.7% among resistant strains and 3% in all strains. The liquid cultures were better for detection of both MTBC and NTMs isolates. The data demonstrate that MTBC continues to be challenge for this country and indicates the need for continued surveillance and full-spectrum services of mycobacteriology laboratory and infectious diseases.

A Current Microbiological Picture of <italic>Mycobacterium</italic> Isolates from Istanbul, Turkey

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A Current Microbiological Picture of  Isolates from Istanbul, Turkey

Despite advances in diagnosis and treatment, tuberculosis (TB) continues to be one of the essential health problems throughout the world. Turkey is considered...

	2015(n : 14)	2016(n : 45)	2017(n : 66)	2018(n : 78)	2019(n : 30)	Total(n : 233, %)
Sensitive to all drugs	11	42	49	61	19	182	(78.1)
INH	2	3	13	12	9	39	(16.7)
SM	2	1	11	12	8	34	(14.6)
RIF	1	–	2	2	2	7	(3)
EMB	–	–	–	1	1	2	(0.8)
İNH + SM	2	1	8	7	5	23	(9.9)
INH + RIF + SM (MDR-TB)	1		2	2	2	7	(3)

		AFB	MTBC	NTMs
Respiratory	198	98	159	39
Sputum	157	89	120	37
Bronchoalveolar lavage	37	7	35	2
Tracheal aspirate	4	2	4	–
Tissue	22	3	21	1
Lymph Node Biopsy	12	2	12	–
Other Tissues	10	1	9	1
Sterile body fluid	22	–	22	–
Pleura	13	–	13	–
BOS	6	–	6	–
Peritoneum	3	–	3	–
Abscess	18	3	17	1
Fasting gastric fluid	11	–	9	2
Urine	7	–	5	2
Total	278	104	233	45

A Current Microbiological Picture of Mycobacterium Isolates from Istanbul, Turkey

Article Category: original-paper

Pubblicato online: 29 mag 2020

Pagine: 185 - 191

Ricevuto: 13 gen 2020

Accettato: 03 apr 2020

DOI: https://doi.org/10.33073/pjm-2020-021

Parole chiave
tuberculosis, species, complex, culture, drug resistance

© 2020 Bilge Sumbul et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Number and proportion of resistant MTBC strains by years.

Number, species, AFB positivity and distribution of Mycobacterial strains according to clinical samples.

A Current Microbiological Picture of Mycobacterium Isolates from Istanbul, Turkey

Article Category: original-paper

Pubblicato online: 29 mag 2020

Pagine: 185 - 191

Ricevuto: 13 gen 2020

Accettato: 03 apr 2020

DOI: https://doi.org/10.33073/pjm-2020-021

Parole chiavetuberculosis, species, complex, culture, drug resistance

© 2020 Bilge Sumbul et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Number and proportion of resistant MTBC strains by years.

Number, species, AFB positivity and distribution of Mycobacterial strains according to clinical samples.

Parole chiave
tuberculosis, species, complex, culture, drug resistance