<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Two-dimensional gel electrophoresis coupled to protein microarray analysis was used to examine, for the first time, the molecular mechanisms of grapevine (<italic>Vitis vinifera</italic> L., cv. Limberger) habituation. The examination of 2-D maps derived from control and habituated cell culture revealed the presence of 55 protein spots displaying a differential expression pattern. These facts have provide a molecular evidence suggesting that the habituated cells can be used as a model for study of cell differentiation and plant defense mechanisms. Cell death, extra-cellular alkalinization and expression of genes responsible for the formation of the defense-related proteins were analyzed in suspension cultures with hormonal autonomy (habituation). Results obtained using habituated grapevine cells compared with non-habituated cells were different and strongly depended on the concentration of elicitor applied.</p></abstract>

Two-dimensional gel electrophoresis coupled to protein microarray analysis was used to examine, for the first time, the molecular mechanisms of grapevine (Vitis vinifera L., cv. Limberger) habituation. The examination of 2-D maps derived from control and habituated cell culture revealed the presence of 55 protein spots displaying a differential expression pattern. These facts have provide a molecular evidence suggesting that the habituated cells can be used as a model for study of cell differentiation and plant defense mechanisms. Cell death, extra-cellular alkalinization and expression of genes responsible for the formation of the defense-related proteins were analyzed in suspension cultures with hormonal autonomy (habituation). Results obtained using habituated grapevine cells compared with non-habituated cells were different and strongly depended on the concentration of elicitor applied.

Proteomic Insight Into the Molecular Principles of Grapevine Habituation

Plant Production Research Center Piešťany

Slovak University of Technology Bratislava

Agriculture (Pol'nohospodárstvo)

{"article-title":"Proteomic Insight Into the Molecular Principles of Grapevine Habituation"}

Two-dimensional gel electrophoresis coupled to protein microarray analysis was used to examine, for the first time, the molecular mechanisms of grapevine...

Proteomic Insight Into the Molecular Principles of Grapevine Habituation

Pubblicato online: 25 dic 2011

Pagine: 129 - 136

DOI: https://doi.org/10.2478/v10207-011-0013-0

Parole chiave
habituation, L., elicitor, enzyme activity, proteome profiling

This content is open access.

Proteomic Insight Into the Molecular Principles of Grapevine Habituation

Pubblicato online: 25 dic 2011

Pagine: 129 - 136

DOI: https://doi.org/10.2478/v10207-011-0013-0

Parole chiavehabituation, L., elicitor, enzyme activity, proteome profiling

This content is open access.

Parole chiave
habituation, L., elicitor, enzyme activity, proteome profiling