<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The multicenter ENDOBACT study aimed at implementing molecular methods for identification of bacterial species encountered in infective endocarditis, and at attempting to reduce the number of cases with unknown etiology. For eighty seven cases was established a diagnosis of definite infective endocarditis. Thirty two of these cases had negative blood cultures. For nine cases out of 32, valve pieces were available and an attempt was made to identify the etiological agent by molecular techniques. Thirty seven available isolates were identified by phenotypical and molecular comparative methods: 16S rRNA (all available isolates), rpoB (staphylococci, streptococci and enterococci), sodA (streptococci and enterococci) genes sequencing. For eight isolates, the comparative results were discrepant. Species identification of one coagulase negative staphylococcal strain was assigned using molecular methods. Molecular identification methods applied here might represent an added value for clinical and conventional microbiological diagnosis of infective endocarditis in Romania.</p></abstract>

The multicenter ENDOBACT study aimed at implementing molecular methods for identification of bacterial species encountered in infective endocarditis, and at attempting to reduce the number of cases with unknown etiology. For eighty seven cases was established a diagnosis of definite infective endocarditis. Thirty two of these cases had negative blood cultures. For nine cases out of 32, valve pieces were available and an attempt was made to identify the etiological agent by molecular techniques. Thirty seven available isolates were identified by phenotypical and molecular comparative methods: 16S rRNA (all available isolates), rpoB (staphylococci, streptococci and enterococci), sodA (streptococci and enterococci) genes sequencing. For eight isolates, the comparative results were discrepant. Species identification of one coagulase negative staphylococcal strain was assigned using molecular methods. Molecular identification methods applied here might represent an added value for clinical and conventional microbiological diagnosis of infective endocarditis in Romania.

Contributions of ENDOBACT multicentric study to the infective endocarditis etiology in Romania

“Cantacuzino” National Institute for Research and Development in Microbiology and Immunology, Splaiul Independentei, No.103, 050096, Bucharest, Romania, Tel.: +40 213 069 223, Fax: +40 213 069 307

“Cantacuzino” National Institute of Research-Development for Microbiology and Immunology, Molecular Epidemiology Laboratory, Bucharest, Romania

“Cantacuzino” National Institute of Research-Development for Microbiology and Immunology, Vector-Borne Infections Laboratory, Bucharest, Romania

“Prof. C.C. Iliescu” Emergency Institute of Cardiovascular Diseases, Microbiology Laboratory, Bucharest, Romania

Clinical Hospital for Infectious Diseases Cluj-Napoca, Microbiology Laboratory, Cluj-Napoca, Romania

Revista Romana de Medicina de Laborator

The multicenter ENDOBACT study aimed at implementing molecular methods for identification of bacterial species encountered in infective endocarditis, and at...

Contributions of ENDOBACT multicentric study to the infective endocarditis etiology in Romania

Pubblicato online: 21 giu 2013

Pagine: 197 - 208

DOI: https://doi.org/10.2478/rrlm-2013-0005

This content is open access.