Murine hepatic proteome adaptation to high-fat diets with different contents of saturated fatty acids and linoleic acid : α-linolenic acid polyunsaturated fatty acid ratios
Kamila P. Liput
- Department of Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, JastrzębiecMagdalenka, Poland
- Institute of Biochemistry and Biophysics of the Polish Academy of SciencesWarsaw, Poland
Profilo Orcid, Adam Lepczyński
Profilo Orcid, Ewa Poławska
Profilo Orcid, Magdalena Ogłuszka
Profilo Orcid, Rafał Starzyński
Profilo Orcid, Paweł Urbański
Profilo Orcid, Agata Nawrocka
Profilo Orcid, Aneta Jończy
Profilo Orcid, , , , Grzegorz Woźniakowski
- Department of Infectious and Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus UniversityToruń, Poland
- Division of Functional Genomics in Biological and Biomedical Research, Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus UniversityToruń, Poland
Profilo Orcid, e Mariusz Pierzchała
Profilo Orcid 
Pubblicato online: 08 ago 2024
Pagine: 427 - 441
Ricevuto: 30 gen 2024
Accettato: 29 lug 2024
DOI: https://doi.org/10.2478/jvetres-2024-0041
Parole chiave
© 2024 Kamila P. Liput et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Some health disorders, such as obesity and type 2 diabetes, are associated with a poor diet and low quality of the fat in it. The type and duration of the diet have an impact on the liver. This investigation uses the proteomic approach to identify changes in the mouse liver protein profile in adaptation to high-fat diets with different saturated fatty acid contents and linoleic acid (18:2
Material and Methods
Four groups of male mice were fed different diets: one standard diet and three high-fat diets were investigated. After six months on these diets, the animals were sacrificed for liver dissection. Two-dimensional electrophoresis was used to separate the complex liver protein mixture, which enabled the separation of proteins against a wide, 3–10 range of pH and molecular weights of 15–250 kDa. Protein profiles were analysed in the PDQuest Advanced 8.0.1 program. Differentially expressed spots were identified using matrix-assisted laser desorption/ionisation–time-of-flight tandem mass spectrometry and peptide mass fingerprinting. The levels of identified proteins were validated using Western blotting. Transcript levels were evaluated using a real-time quantitative PCR.
Results
The analysis of mouse liver protein profiles enabled the identification of 32 protein spots differing between nutritional groups.
Conclusion
A diet high in polyunsaturated fatty acids modulated the levels of liver proteins involved in critical metabolic pathways, including amino acid metabolism, carbohydrate metabolism and cellular response to oxidative stress.