Fish species | Sample | PCRs |
Nested PCRs |
Sequence analysis (300 base pairs) | Remarks |
---|---|---|---|---|---|
Goldfish | Gill swabs and leukocytes | +/+ | 99% koi herpesvirus | Herpesvirus-positive | |
Crucian carp | +/+ | 99% koi herpesvirus | |||
Koi | +/- | 99% goldfish herpesvirus | |||
Tilapia | All negative | +/- | 98% goldfish herpesvirus | ||
Grass carp | Gill swabs only |
+/- | 82% carp pox virus | ||
78% koi herpesvirus | |||||
78% goldfish herpesvirus | |||||
79% sichel herpesvirus |
Fish species (n=4 for each) | New PCR for GHV | Bercovier |
Engelsma |
Assessments |
---|---|---|---|---|
Goldfish | 0/4 | 2/4 | 2/4 | KHV |
Crucian carp | 1/4 | 4/4 | 4/4 | GHV / KHV |
Koi | 0/4 | 3/4 | 1/4 | KHV |
Tilapia | 0/4 | 4/4 | 1/4 | KHV |
Grass carp | 0/4 | 3/4 | 3/4 | KHV |
Fish species | Detected at 0 dpi | Detected in pooled samples | Overall assessments |
---|---|---|---|
Goldfish | KHV | GHV and KHV | Double infection |
Crucian carp | KHV | GHV and KHV | Double infection |
Koi | GHV | negative |
Double infection despite negative result |
Tilapia | GHV | GHV | Double infection |
Grass carp | new HV | GHV | Triple infection |
Fish species | New GHV PCR | Bercovier |
Engelsma |
Assessments |
---|---|---|---|---|
Goldfish | 0/6 | 1/6 | 0/6 | KHV |
Crucian carp | 5/6 | 1/6 | 5/6 | GHV/KHV |
Koi | 0/7 | 0/7 | 0/7 | negative |
Tilapia | 0/6 | 1/6 | 1/6 | KHV |
Grass carp | 0/2 | 0/2 | 2/2 | new HV |