<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>The aim of the study was to determine the genetic diversity of <italic>Echinococcus multilocularis</italic> in pigs in highly endemic areas in Poland, as well as to attempt to confirm the occurrence and geographical distribution of haplotypes characteristic for these areas, which were previously described on the basis of examination of adult tapeworms isolated from foxes.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>Twenty samples of <italic>E. multilocularis</italic> larval forms were obtained from pigs’ livers in four provinces of Poland. Genetic analyses were conducted on sequences of two mitochondrial genes: <italic>cox1</italic> and <italic>nad2</italic>.</p>
</sec>
<sec>
<h3>Results</h3>
<p>Seven haplotypes were found for the <italic>cox1</italic> gene (OQ874673–OQ874679) and four haplotypes for <italic>nad2</italic> (OQ884981–OQ884984). They corresponded to the haplotypes described earlier in foxes in Poland (some of them differing only in one nucleotide). The analysis showed the presence of the Asian-like haplotype in both the <italic>cox1</italic> and <italic>nad2</italic> genes. The remaining haplotypes were grouped in the European clade. The geographical distribution of haplotypes identified in the pig samples was noticed to bear a similarity to the distribution of haplotypes previously isolated from foxes in the same regions.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>The characteristic geographical distribution of <italic>E. multilocularis</italic> haplotypes in Central Europe (including the presence of the Asian-like haplotype) previously described in the population of definitive hosts (foxes) has now been confirmed by the analysis of samples from non-specific intermediate hosts (pigs).</p>
</sec>
</abstract>

Introduction
The aim of the study was to determine the genetic diversity of Echinococcus multilocularis in pigs in highly endemic areas in Poland, as well as to attempt to confirm the occurrence and geographical distribution of haplotypes characteristic for these areas, which were previously described on the basis of examination of adult tapeworms isolated from foxes.


Material and Methods
Twenty samples of E. multilocularis larval forms were obtained from pigs’ livers in four provinces of Poland. Genetic analyses were conducted on sequences of two mitochondrial genes: cox1 and nad2.


Results
Seven haplotypes were found for the cox1 gene (OQ874673–OQ874679) and four haplotypes for nad2 (OQ884981–OQ884984). They corresponded to the haplotypes described earlier in foxes in Poland (some of them differing only in one nucleotide). The analysis showed the presence of the Asian-like haplotype in both the cox1 and nad2 genes. The remaining haplotypes were grouped in the European clade. The geographical distribution of haplotypes identified in the pig samples was noticed to bear a similarity to the distribution of haplotypes previously isolated from foxes in the same regions.


Conclusion
The characteristic geographical distribution of E. multilocularis haplotypes in Central Europe (including the presence of the Asian-like haplotype) previously described in the population of definitive hosts (foxes) has now been confirmed by the analysis of samples from non-specific intermediate hosts (pigs).

<italic>Echinococcus multilocularis</italic> genetic diversity based on isolates from pigs confirmed the characteristic haplotype distribution and the presence of the Asian-like haplotype in Central Europe

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 genetic diversity based on isolates from pigs confirmed the characteristic haplotype distribution and the presence of the Asian-like haplotype in Central Europe

Introduction
The aim of the study was to determine the genetic diversity of Echinococcus multilocularis in pigs in highly endemic areas in Poland, as well as...

Echinococcus multilocularis genetic diversity based on isolates from pigs confirmed the characteristic haplotype distribution and the presence of the Asian-like haplotype in Central Europe

Pubblicato online: 19 dic 2023

Pagine: 567 - 574

Ricevuto: 19 mag 2023

Accettato: 04 ott 2023

DOI: https://doi.org/10.2478/jvetres-2023-0056

Parole chiave
pigs, haplotypes, genetic diversity

© 2023 Jacek Karamon et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Echinococcus multilocularis genetic diversity based on isolates from pigs confirmed the characteristic haplotype distribution and the presence of the Asian-like haplotype in Central Europe

Pubblicato online: 19 dic 2023

Pagine: 567 - 574

Ricevuto: 19 mag 2023

Accettato: 04 ott 2023

DOI: https://doi.org/10.2478/jvetres-2023-0056

Parole chiavepigs, haplotypes, genetic diversity

© 2023 Jacek Karamon et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
pigs, haplotypes, genetic diversity