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Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by Staphylococcus aureus

Wenjia Wang

Wenjia Wang

  • College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
  • College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyZhengzhou, PR China
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Wang, Wenjia
, 
Rongling Li

Rongling Li

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Li, Rongling
, 
Tingzhu Ye

Tingzhu Ye

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Ye, Tingzhu
, 
Xinxin Zhang

Xinxin Zhang

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Zhang, Xinxin
, 
Chao Chen

Chao Chen

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Chen, Chao
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Ai-xin Liang

Ai-xin Liang

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Liang, Ai-xin
 e 
Li-guo Yang

Li-guo Yang

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Yang, Li-guo
  
05 mag 2022
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Pubblicato online: 05 mag 2022
Pagine: 179 - 187
Ricevuto: 23 nov 2021
Accettato: 21 apr 2022
DOI: https://doi.org/10.2478/jvetres-2022-0022
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© 2022 W. Wang et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Identification of Staphylococcus aureus isolated from milk samples from Holstein cows. A − specific PCR and electrophoresis map of the S. aureus protein A (spa) gene (323 bp); B − morphology of S. aureus colony on Baird-Parker agar
Identification of Staphylococcus aureus isolated from milk samples from Holstein cows. A − specific PCR and electrophoresis map of the S. aureus protein A (spa) gene (323 bp); B − morphology of S. aureus colony on Baird-Parker agar

Fig. 2

Transcriptome profiles of 16,544 genes identified by mRNA in peripheral blood leukocytes (PBL) from all the samples (A); volcano plot of global differentially expressed genes (DEGs) in PBL between the mastitic (M) and non-mastitic (NM) groups (B); and transcriptome profiles of 4,286 DEGs between the M and NM groups (C)
Transcriptome profiles of 16,544 genes identified by mRNA in peripheral blood leukocytes (PBL) from all the samples (A); volcano plot of global differentially expressed genes (DEGs) in PBL between the mastitic (M) and non-mastitic (NM) groups (B); and transcriptome profiles of 4,286 DEGs between the M and NM groups (C)

Fig. 3

The top 30 up- and downregulated differentially expressed genes analysed by Gene Ontology BP – biological processes; CC – cellular components; MF – molecular functions
The top 30 up- and downregulated differentially expressed genes analysed by Gene Ontology BP – biological processes; CC – cellular components; MF – molecular functions

Fig. 4

Pathways significantly enriched with the upregulated and downregulated DEGs, as observed by KEGG enrichment
Pathways significantly enriched with the upregulated and downregulated DEGs, as observed by KEGG enrichment

Fig. 5

DEGs downregulated in the metabolic pathway (A) and upregulated in platelet activation (B) observed by protein–protein interaction (PPI) analysis. The different colours represent degrees of connectivity
DEGs downregulated in the metabolic pathway (A) and upregulated in platelet activation (B) observed by protein–protein interaction (PPI) analysis. The different colours represent degrees of connectivity

Fig. 6

Concordance of gene expression profiles obtained by RNA-seq and qRT-PCR methods for four upregulated genes (VWF, ITGB3, F2RL3, and PRKG1) and three downregulated genes (GDA, HNMT, and GPT2). The β-actin gene was used as an internal reference control gene
Concordance of gene expression profiles obtained by RNA-seq and qRT-PCR methods for four upregulated genes (VWF, ITGB3, F2RL3, and PRKG1) and three downregulated genes (GDA, HNMT, and GPT2). The β-actin gene was used as an internal reference control gene

Characteristics of the seven experimental and control dairy cows

Animals Group Lactation (d) SCC (×104/mL) Milk (kg/day) yield Milk morphology Bacterium
NM002 NM 288 4.5 23 normal −
NM003 NM 256 5.1 19 normal −
NM013 NM 241 3.9 18 normal −
NM182 NM 198 4.4 20 normal −
M008 M 267 623 5.0 thin, clots Staphylococcus aureus
M010 M 213 456 4.6 flakes Staphylococcus aureus
M021 M 186 503 3.0 clots Staphylococcus aureus

RNA-seq data for the seven experimental and control dairy cows

Samples Total clean reads (bp) Clean reads Q20 (%) Clean reads Q30 (%) Total mapping ratio (%)
NM002 47,493,260 97.78 91.07 82.86
NM003 47,472,822 97.83 91.31 82.25
NM013 47,646,180 97.87 91.34 83.81
NM182 47,588,374 97.84 91.22 84.97
M008 47,642,372 97.92 91.64 79.93
M010 47,710,376 97.96 91.82 76.37
M021 47,664,652 97.92 91.75 77.52
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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