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Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by Staphylococcus aureus

Wenjia Wang
Wenjia Wang
, 
Rongling Li
Rongling Li
, 
Tingzhu Ye
Tingzhu Ye
, 
Xinxin Zhang
Xinxin Zhang
, 
Chao Chen
Chao Chen
, 
Ai-xin Liang
Ai-xin Liang
 et 
Li-guo Yang
Li-guo Yang
   | 05 mai 2022
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Publié en ligne: 05 mai 2022
Pages: 179 - 187
Reçu: 23 nov. 2021
Accepté: 21 avr. 2022
DOI: https://doi.org/10.2478/jvetres-2022-0022
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© 2022 W. Wang et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Identification of Staphylococcus aureus isolated from milk samples from Holstein cows. A − specific PCR and electrophoresis map of the S. aureus protein A (spa) gene (323 bp); B − morphology of S. aureus colony on Baird-Parker agar
Identification of Staphylococcus aureus isolated from milk samples from Holstein cows. A − specific PCR and electrophoresis map of the S. aureus protein A (spa) gene (323 bp); B − morphology of S. aureus colony on Baird-Parker agar

Fig. 2

Transcriptome profiles of 16,544 genes identified by mRNA in peripheral blood leukocytes (PBL) from all the samples (A); volcano plot of global differentially expressed genes (DEGs) in PBL between the mastitic (M) and non-mastitic (NM) groups (B); and transcriptome profiles of 4,286 DEGs between the M and NM groups (C)
Transcriptome profiles of 16,544 genes identified by mRNA in peripheral blood leukocytes (PBL) from all the samples (A); volcano plot of global differentially expressed genes (DEGs) in PBL between the mastitic (M) and non-mastitic (NM) groups (B); and transcriptome profiles of 4,286 DEGs between the M and NM groups (C)

Fig. 3

The top 30 up- and downregulated differentially expressed genes analysed by Gene Ontology BP – biological processes; CC – cellular components; MF – molecular functions
The top 30 up- and downregulated differentially expressed genes analysed by Gene Ontology BP – biological processes; CC – cellular components; MF – molecular functions

Fig. 4

Pathways significantly enriched with the upregulated and downregulated DEGs, as observed by KEGG enrichment
Pathways significantly enriched with the upregulated and downregulated DEGs, as observed by KEGG enrichment

Fig. 5

DEGs downregulated in the metabolic pathway (A) and upregulated in platelet activation (B) observed by protein–protein interaction (PPI) analysis. The different colours represent degrees of connectivity
DEGs downregulated in the metabolic pathway (A) and upregulated in platelet activation (B) observed by protein–protein interaction (PPI) analysis. The different colours represent degrees of connectivity

Fig. 6

Concordance of gene expression profiles obtained by RNA-seq and qRT-PCR methods for four upregulated genes (VWF, ITGB3, F2RL3, and PRKG1) and three downregulated genes (GDA, HNMT, and GPT2). The β-actin gene was used as an internal reference control gene
Concordance of gene expression profiles obtained by RNA-seq and qRT-PCR methods for four upregulated genes (VWF, ITGB3, F2RL3, and PRKG1) and three downregulated genes (GDA, HNMT, and GPT2). The β-actin gene was used as an internal reference control gene

Characteristics of the seven experimental and control dairy cows

Animals Group Lactation (d) SCC (×104/mL) Milk (kg/day) yield Milk morphology Bacterium
NM002 NM 288 4.5 23 normal −
NM003 NM 256 5.1 19 normal −
NM013 NM 241 3.9 18 normal −
NM182 NM 198 4.4 20 normal −
M008 M 267 623 5.0 thin, clots Staphylococcus aureus
M010 M 213 456 4.6 flakes Staphylococcus aureus
M021 M 186 503 3.0 clots Staphylococcus aureus

RNA-seq data for the seven experimental and control dairy cows

Samples Total clean reads (bp) Clean reads Q20 (%) Clean reads Q30 (%) Total mapping ratio (%)
NM002 47,493,260 97.78 91.07 82.86
NM003 47,472,822 97.83 91.31 82.25
NM013 47,646,180 97.87 91.34 83.81
NM182 47,588,374 97.84 91.22 84.97
M008 47,642,372 97.92 91.64 79.93
M010 47,710,376 97.96 91.82 76.37
M021 47,664,652 97.92 91.75 77.52
eISSN:
2450-8608
Langue:
Anglais
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Sujets de la revue:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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