Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae
Pubblicato online: 10 mar 2022
Pagine: 95 - 101
Ricevuto: 02 nov 2021
Accettato: 23 feb 2022
DOI: https://doi.org/10.2478/jvetres-2022-0010
Parole chiave
© 2022 A. Sánchez et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially
Material and Methods
This study was designed to appraise the performance of two commercial indirect ELISA tests using
Results
All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93).
Conclusion
The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting