<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2022-0010_s_005">
<h3>Introduction</h3>
<p>Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially <italic>M. agalactiae</italic>. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test.</p>
</sec>
<sec id="j_jvetres-2022-0010_s_006">
<h3>Material and Methods</h3>
<p>This study was designed to appraise the performance of two commercial indirect ELISA tests using <italic>M. agalactiae</italic> p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different <italic>M. agalactiae</italic> strains. We carried out the test evaluation using sera of confirmed <italic>M. agalactiae</italic>-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests.</p></sec>
<sec id="j_jvetres-2022-0010_s_007">
<h3>Results</h3>
<p>All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93).</p></sec>
<sec id="j_jvetres-2022-0010_s_008">
<h3>Conclusion</h3>
<p>The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting <italic>M. agalactiae</italic> in milk and presenting clinical signs. The agreements show that any of these ELISA tests could be equally well used for diagnosis in programmes against CA.</p>
</sec>
</abstract>

Introduction
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test.


Material and Methods
This study was designed to appraise the performance of two commercial indirect ELISA tests using M. agalactiae p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different M. agalactiae strains. We carried out the test evaluation using sera of confirmed M. agalactiae-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests.

Results
All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93).

Conclusion
The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting M. agalactiae in milk and presenting clinical signs. The agreements show that any of these ELISA tests could be equally well used for diagnosis in programmes against CA.

Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by <italic>Mycoplasma agalactiae</italic>

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by 

Introduction
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M....

Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae

Pubblicato online: 10 mar 2022

Pagine: 95 - 101

Ricevuto: 02 nov 2021

Accettato: 23 feb 2022

DOI: https://doi.org/10.2478/jvetres-2022-0010

Parole chiave
contagious agalactia, enzyme-linked immunosorbent assays, small ruminants, goat

© 2022 A. Sánchez et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae

Pubblicato online: 10 mar 2022

Pagine: 95 - 101

Ricevuto: 02 nov 2021

Accettato: 23 feb 2022

DOI: https://doi.org/10.2478/jvetres-2022-0010

Parole chiavecontagious agalactia, enzyme-linked immunosorbent assays, small ruminants, goat

© 2022 A. Sánchez et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
contagious agalactia, enzyme-linked immunosorbent assays, small ruminants, goat