Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation

Apocrine sweat gland carcinomas (ASGCs) are rare malignant skin tumours in dogs and humans. The literature published so far focuses mostly on the clinico-epidemiological aspect of these tumours, but little is known about their pathogenesis. In this study we aimed to determine whether the p53 gene is involved in the carcinogenesis of the apocrine sweat gland in dogs and whether ultraviolet radiation (UV) is related to it.

Material and Methods

Forty canine ASGCs were submitted to laser capture microdissection to isolate neoplastic cells, from which DNA was subsequently extracted. PCR amplification and sequencing of p53 exons 2–8 was then performed, followed by computer analysis of the obtained sequences.

Results

Sixteen mutations within the p53 gene were found in 13 tumours. The mutations involved C → T, T → C, G → A, and CC → TT transitions, C → G transversion and adenine deletion, which are gene alteration types known to be related to UV radiation in the process of skin carcinogenesis in humans. Six of the thirteen tumour cases displayed the C → T transitions in the same location in exon 4 and three of the thirteen cases displayed T → C in the same location in exon 5.

Conclusion

The results of the present study indicate both the participation of the p53 gene and the influence of UV radiation in the formation of ASGCs in dogs.

Lingua:: Inglese

Frequenza di pubblicazione:: 4 volte all'anno
Argomenti della rivista:: Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria

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Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation

Agnieszka Jasik

Anna Kycko

Monika Olech

Krzysztof Wyrostek

Anna Śmiech

Wojciech Łopuszyński

Iwona Otrocka-Domagała

Mateusz Mikiewicz

Izabella Dolka

Categoria dell'articolo: Review article

Pubblicato online: 20 dic 2021

Pagine: 519 - 526

Ricevuto: 13 ago 2021

Accettato: 09 dic 2021

DOI: https://doi.org/10.2478/jvetres-2021-0070

Parole chiavedog, apocrine sweat gland carcinoma, gene, mutations, UVR

© 2021 A. Jasik et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Material and Methods

Results

Conclusion

Parole chiave
dog, apocrine sweat gland carcinoma, gene, mutations, UVR