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Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

Marek Gehrke

Marek Gehrke

Centre for Veterinary Sciences at the Nicolaus Copernicus UniversityToruń, Poland
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Gehrke, Marek
, 
Beata Blaszak

Beata Blaszak

Department of Tumour Pathology, Greater Poland Cancer CentrePoznań, Poland
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Blaszak, Beata
, 
Monika Stachowiak

Monika Stachowiak

Department of Genetics and Animal BreedingPoznań, Poland
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Stachowiak, Monika
, 
Izabela Szczerbal

Izabela Szczerbal

Department of Genetics and Animal BreedingPoznań, Poland
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Szczerbal, Izabela
, 
Barbara Stefańska

Barbara Stefańska

Department of Animal Nutrition and Feed Management, Faculty of Veterinary Medicine and Animal Sciences, Poznań University of Life SciencesPoznań, Poland
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Stefańska, Barbara
, 
Jędrzej M. Jaśkowski

Jędrzej M. Jaśkowski

Centre for Veterinary Sciences at the Nicolaus Copernicus UniversityToruń, Poland
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Jaśkowski, Jędrzej M.
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Włodzimierz Nowak

Włodzimierz Nowak

Department of Animal Nutrition and Feed Management, Faculty of Veterinary Medicine and Animal Sciences, Poznań University of Life SciencesPoznań, Poland
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Nowak, Włodzimierz
 e 
Marek Świtoński

Marek Świtoński

Department of Genetics and Animal BreedingPoznań, Poland
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Świtoński, Marek
  
13 set 2019
Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies's Cover Image
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Pubblicato online: 13 set 2019
Pagine: 391 - 398
Ricevuto: 15 ago 2018
Accettato: 07 ago 2019
DOI: https://doi.org/10.2478/jvetres-2019-0057
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© 2019 M. Gehrke et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Amorphus globosus A1 of cow A
Amorphus globosus A1 of cow A

Fig. 2

Amorphus globosus B1 of cow B
Amorphus globosus B1 of cow B

Fig. 3

Amorphus globosus C1 of cow C
Amorphus globosus C1 of cow C

Fig. 4

Amorphus globosus C2 of cow C
Amorphus globosus C2 of cow C

Fig. 5

Skin of A1. The area of black hair, epidermal cells with a layer of keratin, the early developmental stages of hair, and segmental separation of the keratin layer (4×, HE)
Skin of A1. The area of black hair, epidermal cells with a layer of keratin, the early developmental stages of hair, and segmental separation of the keratin layer (4×, HE)

Fig. 6

Wall of internal cavity of B1. The layer of keratin with a few cells getting separated, horizontally running sheaths of hair without cellular elements, absence of cell layers in the epidermis, the dermis with a few cells indicating defragmentation of nuclei, and early stages of hair development (4×, HE)
Wall of internal cavity of B1. The layer of keratin with a few cells getting separated, horizontally running sheaths of hair without cellular elements, absence of cell layers in the epidermis, the dermis with a few cells indicating defragmentation of nuclei, and early stages of hair development (4×, HE)

Fig. 7

Mesenchymal and fat tissue of B1. A dense extracellular matrix devoid of cellular elements (2×, HE)
Mesenchymal and fat tissue of B1. A dense extracellular matrix devoid of cellular elements (2×, HE)

Fig. 8

Connective tissue of C2 with clusters of fat, vessels, and lymphatic tissue. Structural diversity in the vascular walls (first stage of endothelial organisation) (2×, HE)
Connective tissue of C2 with clusters of fat, vessels, and lymphatic tissue. Structural diversity in the vascular walls (first stage of endothelial organisation) (2×, HE)

Fig. 9

In C2, the free spaces with the features of epithelial lining of (10×, HE)
In C2, the free spaces with the features of epithelial lining of (10×, HE)

Fig. 10

Buds of early bone structure of C2 (2×, HE)
Buds of early bone structure of C2 (2×, HE)

Fig. 11

Giemsa-stained metaphase spreads of the viable sibling A-m (60, XY)
Giemsa-stained metaphase spreads of the viable sibling A-m (60, XY)

Fig. 12

Giemsa-stained metaphase spreads of the viable sibling C-f (60, XX)
Giemsa-stained metaphase spreads of the viable sibling C-f (60, XX)

Fig. 13

Electrophoresis of PCR products of theSRY gene (851bp) of A1, B1, and the healthy brother of A1. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female
Electrophoresis of PCR products of theSRY gene (851bp) of A1, B1, and the healthy brother of A1. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female

Fig. 14

Electrophoresis of PCR products of theSRY gene (851bp) of C1, C2, healthy male, and female co-multiples. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male
Electrophoresis of PCR products of theSRY gene (851bp) of C1, C2, healthy male, and female co-multiples. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male

Fig. 15

Electrophoresis of PCR products forAMELX andAMELY genes of A1, B1, and healthy brother of A1. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in the female. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female
Electrophoresis of PCR products forAMELX andAMELY genes of A1, B1, and healthy brother of A1. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in the female. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female

Fig. 16

Electrophoresis of PCR products forAMELX andAMELY genes of C1, C2, and healthy male and female co-multiples. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in females. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male
Electrophoresis of PCR products forAMELX andAMELY genes of C1, C2, and healthy male and female co-multiples. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in females. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male

Genetic analysis of the amorphic foetuses and their full siblings

Genes
Multiplets Individuals Karyotype SRY AMELX AMELY STR (17 loci)
Normal male (A-m) 60, XY + + + The same genotype as A1
A Ag -(A1) + + + The same genotype as co-twin
Stillborn male na* na na na na
B Stillborn male na na na na na
Ag -(B1) + + + na
Normal male (C-m) 60, XY + + + Genotype different from C-f, C1, and C2
Normal female (C-f) 60, XX − + − The same genotype as C1 and C2
C Ag -(C1) − + − The same genotype as C-f and C2
Ag-(C2) − + − The same genotype as C-f and C1
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Inglese
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Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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