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Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

Marek Gehrke
Marek Gehrke
, 
Beata Blaszak
Beata Blaszak
, 
Monika Stachowiak
Monika Stachowiak
, 
Izabela Szczerbal
Izabela Szczerbal
, 
Barbara Stefańska
Barbara Stefańska
, 
Jędrzej M. Jaśkowski
Jędrzej M. Jaśkowski
, 
Włodzimierz Nowak
Włodzimierz Nowak
 and 
Marek Świtoński
Marek Świtoński
   | Sep 13, 2019
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Published Online: Sep 13, 2019
Page range: 391 - 398
Received: Aug 15, 2018
Accepted: Aug 07, 2019
DOI: https://doi.org/10.2478/jvetres-2019-0057
Keywords
© 2019 M. Gehrke et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Amorphus globosus A1 of cow A
Amorphus globosus A1 of cow A

Fig. 2

Amorphus globosus B1 of cow B
Amorphus globosus B1 of cow B

Fig. 3

Amorphus globosus C1 of cow C
Amorphus globosus C1 of cow C

Fig. 4

Amorphus globosus C2 of cow C
Amorphus globosus C2 of cow C

Fig. 5

Skin of A1. The area of black hair, epidermal cells with a layer of keratin, the early developmental stages of hair, and segmental separation of the keratin layer (4×, HE)
Skin of A1. The area of black hair, epidermal cells with a layer of keratin, the early developmental stages of hair, and segmental separation of the keratin layer (4×, HE)

Fig. 6

Wall of internal cavity of B1. The layer of keratin with a few cells getting separated, horizontally running sheaths of hair without cellular elements, absence of cell layers in the epidermis, the dermis with a few cells indicating defragmentation of nuclei, and early stages of hair development (4×, HE)
Wall of internal cavity of B1. The layer of keratin with a few cells getting separated, horizontally running sheaths of hair without cellular elements, absence of cell layers in the epidermis, the dermis with a few cells indicating defragmentation of nuclei, and early stages of hair development (4×, HE)

Fig. 7

Mesenchymal and fat tissue of B1. A dense extracellular matrix devoid of cellular elements (2×, HE)
Mesenchymal and fat tissue of B1. A dense extracellular matrix devoid of cellular elements (2×, HE)

Fig. 8

Connective tissue of C2 with clusters of fat, vessels, and lymphatic tissue. Structural diversity in the vascular walls (first stage of endothelial organisation) (2×, HE)
Connective tissue of C2 with clusters of fat, vessels, and lymphatic tissue. Structural diversity in the vascular walls (first stage of endothelial organisation) (2×, HE)

Fig. 9

In C2, the free spaces with the features of epithelial lining of (10×, HE)
In C2, the free spaces with the features of epithelial lining of (10×, HE)

Fig. 10

Buds of early bone structure of C2 (2×, HE)
Buds of early bone structure of C2 (2×, HE)

Fig. 11

Giemsa-stained metaphase spreads of the viable sibling A-m (60, XY)
Giemsa-stained metaphase spreads of the viable sibling A-m (60, XY)

Fig. 12

Giemsa-stained metaphase spreads of the viable sibling C-f (60, XX)
Giemsa-stained metaphase spreads of the viable sibling C-f (60, XX)

Fig. 13

Electrophoresis of PCR products of theSRY gene (851bp) of A1, B1, and the healthy brother of A1. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female
Electrophoresis of PCR products of theSRY gene (851bp) of A1, B1, and the healthy brother of A1. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female

Fig. 14

Electrophoresis of PCR products of theSRY gene (851bp) of C1, C2, healthy male, and female co-multiples. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male
Electrophoresis of PCR products of theSRY gene (851bp) of C1, C2, healthy male, and female co-multiples. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male

Fig. 15

Electrophoresis of PCR products forAMELX andAMELY genes of A1, B1, and healthy brother of A1. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in the female. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female
Electrophoresis of PCR products forAMELX andAMELY genes of A1, B1, and healthy brother of A1. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in the female. Lanes: 1 – molecular weight marker; 2 – abnormal foetus B1; 3 – abnormal foetus A1; 4 – healthy brother of abnormal foetus A1; 5 – reference male; 6 – reference female

Fig. 16

Electrophoresis of PCR products forAMELX andAMELY genes of C1, C2, and healthy male and female co-multiples. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in females. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male
Electrophoresis of PCR products forAMELX andAMELY genes of C1, C2, and healthy male and female co-multiples. Two bands (280 bp and 217 bp) are visible in males and a single band (280 bp) in females. Lanes: 1 – molecular weight marker; 2 – healthy brother; 3 – healthy sister; 4 – abnormal foetus C1; 5 – abnormal foetus C2; 6 – reference female; 7 – reference male

Genetic analysis of the amorphic foetuses and their full siblings

Genes
Multiplets Individuals Karyotype SRY AMELX AMELY STR (17 loci)
Normal male (A-m) 60, XY + + + The same genotype as A1
A Ag -(A1) + + + The same genotype as co-twin
Stillborn male na* na na na na
B Stillborn male na na na na na
Ag -(B1) + + + na
Normal male (C-m) 60, XY + + + Genotype different from C-f, C1, and C2
Normal female (C-f) 60, XX − + − The same genotype as C1 and C2
C Ag -(C1) − + − The same genotype as C-f and C2
Ag-(C2) − + − The same genotype as C-f and C1
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