Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies
Pubblicato online: 13 set 2019
Pagine: 391 - 398
Ricevuto: 15 ago 2018
Accettato: 07 ago 2019
DOI: https://doi.org/10.2478/jvetres-2019-0057
Parole chiave
© 2019 M. Gehrke et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
A comprehensive description is presented of four novel cases of
Material and Methods
Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and from blood leukocytes of their healthy siblings. PCR reactions were used to reveal the presence of Y-linked genes (SRY and AMELY) and an X-linked gene (AMELX).
Results
All foetuses were classified to the group
Conclusion
Foetuses had monozygotic origin. Histological analysis showed a low level of development of tissues of meso- and ectodermal origin, as well as features of degrading patterns.