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Detection and Molecular Characterization of Peach Latent Mosaic Viroid Isolates Infecting Stone Fruit Trees in Poland

Mirosława Cieślińska

Mirosława Cieślińska

The National Institute of Horticultural ResearchPoland
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Cieślińska, Mirosława
  
29 dic 2023
Detection and Molecular Characterization of Peach Latent Mosaic Viroid Isolates Infecting Stone Fruit Trees in Poland's Cover Image
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Pubblicato online: 29 dic 2023
Pagine: 63 - 68
Ricevuto: 01 lug 2023
Accettato: 01 dic 2023
DOI: https://doi.org/10.2478/johr-2023-0036
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© 2023 Mirosława Cieślińska, published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1.

Yellow mosaic on the leaf of ‘Maycrest’ peach infected by PLMVd
Yellow mosaic on the leaf of ‘Maycrest’ peach infected by PLMVd

Figure 2.

Fruit of PLMVd infected ‘Maycrest’ peach with cracked, corky sutures and enlarged pits
Fruit of PLMVd infected ‘Maycrest’ peach with cracked, corky sutures and enlarged pits

Figure 3.

An unrooted phylogenetic tree constructed using the maximum likelihood method based on analyzed Polish peach latent mosaic viroid isolates and selected sequences available in GenBank Isolates are described by Acc. No., host plant, and country of origin. Isolates analyzed in this study are marked in bold. Numbers at the nodes are bootstrap values based on 1,000 repetitions. Only values above 70 are shown
An unrooted phylogenetic tree constructed using the maximum likelihood method based on analyzed Polish peach latent mosaic viroid isolates and selected sequences available in GenBank Isolates are described by Acc. No., host plant, and country of origin. Isolates analyzed in this study are marked in bold. Numbers at the nodes are bootstrap values based on 1,000 repetitions. Only values above 70 are shown

Figure 4.

Dot-blot analysis with a PLMVd-specific digoxigenin-labeled riboprobe of RNA extracted from stone fruit treesNegative control (A1), positive control (A2), P-Vioyt (A3), A-Mord (A4), P-WB258 (A5), P-Relia (A6), P-EOra (A7), P-Inka (B1), A-Harc (B2), P-ERed (B3), P-Mayc (B4), Pl-OzPr (B5), N-Satu (B6), water (B7)
Dot-blot analysis with a PLMVd-specific digoxigenin-labeled riboprobe of RNA extracted from stone fruit treesNegative control (A1), positive control (A2), P-Vioyt (A3), A-Mord (A4), P-WB258 (A5), P-Relia (A6), P-EOra (A7), P-Inka (B1), A-Harc (B2), P-ERed (B3), P-Mayc (B4), Pl-OzPr (B5), N-Satu (B6), water (B7)

Characterization of the peach latent mosaic viroid isolates used in this study

Isolate Host species, cultivar/clone Origin Genome size (bp) GeneBank Accession No.
Inka peach, Inka Świętokrzyskie 337 MW928673.1
Harc apricot, Harcot Dolnośląskie 337 MW928674.1
Satu nectarine, Saturn Łódzkie 337 MW928675.1
Mayc peach, Maycrest Łódzkie 337 MW928676.1
OzPr Japanese plum, Ozark Premier Peach Lubelskie 337 MW928677.1
EOra Early Orange Dolnośląskie 337 MW928678.1
Mord apricot, Morden Early Świętokrzyskie 338 MW928679.1
Vioyt peach, unknown Łódzkie 338 MW928680.1
WB258 peach, clone Łódzkie 337 MW928681.1
ERed peach, Early Redhaven Świętokrzyskie 338 MW928682.1
Relia peach, Reliance Lubelskie 338 MW928683.1
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