<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Field surveys were carried out to assess the incidence of peach latent mosaic viroid in nine orchards of stone fruit trees in four growing regions of Poland. Forty of 119 peaches, six of 37 apricots, five of eight nectarines and one of four plums trees tested positive by RT-PCR for this viroid. Most of the infected stone fruit trees did not show any symptoms. Leaf yellow mosaic was only noticed on ‘Maycrest’ peach and ‘Saturn’ nectarine trees, and additional symptoms of cracked fruits with corky sutures and enlarged pits were observed on one ‘Maycrest’ tree. One or two isolates from each orchard and each species of infected tree were selected, and together, 11 isolates of peach latent mosaic viroid were used for genetic analyses. The nucleotide sequence similarity of selected isolates found in Poland was 93.6–100%. They shared 82.0–98.8% similarity with the viroid strains from different hosts and geographical regions. The phylogenetic analysis led to the clustering of all isolates detected during this study in the most numerous represented subgroup II-A, composed of peach latent mosaic viroid strains originating from different geographic regions and host plants. Although Polish isolates were clustered in the same subgroup, some differed in length and nucleotide sequences. It was shown that the genetic variability of isolates found in Poland was unrelated to the regions of their origin. A positive signal was obtained in dot-blot hybridization of all analyzed 11 isolates.</p>
</abstract>

Field surveys were carried out to assess the incidence of peach latent mosaic viroid in nine orchards of stone fruit trees in four growing regions of Poland. Forty of 119 peaches, six of 37 apricots, five of eight nectarines and one of four plums trees tested positive by RT-PCR for this viroid. Most of the infected stone fruit trees did not show any symptoms. Leaf yellow mosaic was only noticed on ‘Maycrest’ peach and ‘Saturn’ nectarine trees, and additional symptoms of cracked fruits with corky sutures and enlarged pits were observed on one ‘Maycrest’ tree. One or two isolates from each orchard and each species of infected tree were selected, and together, 11 isolates of peach latent mosaic viroid were used for genetic analyses. The nucleotide sequence similarity of selected isolates found in Poland was 93.6–100%. They shared 82.0–98.8% similarity with the viroid strains from different hosts and geographical regions. The phylogenetic analysis led to the clustering of all isolates detected during this study in the most numerous represented subgroup II-A, composed of peach latent mosaic viroid strains originating from different geographic regions and host plants. Although Polish isolates were clustered in the same subgroup, some differed in length and nucleotide sequences. It was shown that the genetic variability of isolates found in Poland was unrelated to the regions of their origin. A positive signal was obtained in dot-blot hybridization of all analyzed 11 isolates.

Detection and Molecular Characterization of Peach Latent Mosaic Viroid Isolates Infecting Stone Fruit Trees in Poland

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution 4.0 International License.

Field surveys were carried out to assess the incidence of peach latent mosaic viroid in nine orchards of stone fruit trees in four growing regions of Poland....

Isolate	Host species, cultivar/clone	Origin	Genome size (bp)	GeneBank Accession No.
Inka	peach, Inka	Świętokrzyskie	337	MW928673.1
Harc	apricot, Harcot	Dolnośląskie	337	MW928674.1
Satu	nectarine, Saturn	Łódzkie	337	MW928675.1
Mayc	peach, Maycrest	Łódzkie	337	MW928676.1
OzPr	Japanese plum, Ozark Premier Peach	Lubelskie	337	MW928677.1
EOra	Early Orange	Dolnośląskie	337	MW928678.1
Mord	apricot, Morden Early	Świętokrzyskie	338	MW928679.1
Vioyt	peach, unknown	Łódzkie	338	MW928680.1
WB258	peach, clone	Łódzkie	337	MW928681.1
ERed	peach, Early Redhaven	Świętokrzyskie	338	MW928682.1
Relia	peach, Reliance	Lubelskie	338	MW928683.1

Detection and Molecular Characterization of Peach Latent Mosaic Viroid Isolates Infecting Stone Fruit Trees in Poland

Published Online: Dec 29, 2023

Page range: 63 - 68

Received: Jul 01, 2023

Accepted: Dec 01, 2023

DOI: https://doi.org/10.2478/johr-2023-0036

Keywords
PLMVd, sequence analysis, phylogenetic analysis, dot-blot hybridization

© 2023 Mirosława Cieślińska, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Characterization of the peach latent mosaic viroid isolates used in this study

Detection and Molecular Characterization of Peach Latent Mosaic Viroid Isolates Infecting Stone Fruit Trees in Poland

Published Online: Dec 29, 2023

Page range: 63 - 68

Received: Jul 01, 2023

Accepted: Dec 01, 2023

DOI: https://doi.org/10.2478/johr-2023-0036

KeywordsPLMVd, sequence analysis, phylogenetic analysis, dot-blot hybridization

© 2023 Mirosława Cieślińska, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Characterization of the peach latent mosaic viroid isolates used in this study

Keywords
PLMVd, sequence analysis, phylogenetic analysis, dot-blot hybridization